Rabbit Recombinant Monoclonal gamma Catenin antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human, Dog samples.
Images
![Western blot - Anti-gamma Catenin antibody [EPR17310] - BSA and Azide free (AB238447), expandable thumbnail](https://content.abcam.com/products/images/gamma-catenin-antibody-epr17310-bsa-and-azide-free-ab238447--western-blot-img6193.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-gamma Catenin antibody [EPR17310] - BSA and Azide free (AB238447)")
![Flow Cytometry (Intracellular) - Anti-gamma Catenin antibody [EPR17310] - BSA and Azide free (AB238447), expandable thumbnail](https://content.abcam.com/products/images/gamma-catenin-antibody-epr17310-bsa-and-azide-free-ab238447--flow-cytometry-intracellular-img137326.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-gamma Catenin antibody [EPR17310] - BSA and Azide free (AB238447)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310] - BSA and Azide free (AB238447), expandable thumbnail](https://content.abcam.com/products/images/gamma-catenin-antibody-epr17310-bsa-and-azide-free-ab238447--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img94192.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310] - BSA and Azide free (AB238447)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310] - BSA and Azide free (AB238447), expandable thumbnail](https://content.abcam.com/products/images/gamma-catenin-antibody-epr17310-bsa-and-azide-free-ab238447--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img93192.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310] - BSA and Azide free (AB238447)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310] - BSA and Azide free (AB238447), expandable thumbnail](https://content.abcam.com/products/images/gamma-catenin-antibody-epr17310-bsa-and-azide-free-ab238447--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img108465.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310] - BSA and Azide free (AB238447)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected | Tested |
| Rat | Tested | Expected | Expected | Tested |
| Dog | Tested | Tested | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Dog | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Dog | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Dog | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Dog | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
1 product for Alternative Version
Target data
Function
Common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE-cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton (By similarity).
Alternative names
CTNNG, DP3, JUP, Junction plakoglobin, Catenin gamma, Desmoplakin III, Desmoplakin-3
Recommended products
Rabbit Recombinant Monoclonal gamma Catenin antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human, Dog samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR17310
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab238447 is the carrier-free version of Anti-gamma Catenin antibody [EPR17310] ab184919.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Gamma Catenin also known as Junction Plakoglobin (JUP) is a protein with a molecular mass of approximately 82 kDa. It plays an important role in cell adhesion mechanisms. This protein is expressed widely in various tissue types prominently in epithelial tissues. Gamma Catenin contributes significantly to the structure of adherens junctions and desmosomes physically connecting cells to one another and stabilizing those connections within tissues.
- Biological function summary
Gamma Catenin is an integral component of the armadillo family of proteins and part of desmosomal and adherens junction complexes. It plays an essential role in maintaining the integrity and mechanical strength of tissues. Through interacting with other proteins in these complexes gamma Catenin facilitates cell signaling and communication. It associates with cadherins and catenins including plakophilin and β-catenin to ensure proper cell-cell adhesion and signaling across the cell membrane.
- Pathways
Gamma Catenin is involved in the Wnt signaling pathway an important route governing cellular processes such as proliferation differentiation and migration. Gamma Catenin interacts with β-catenin in this pathway influencing transcriptional regulation in the nucleus. It also plays roles in the cadherin-mediated cell adhesion pathway working alongside other proteins like E-cadherin to modulate cell behavior. These pathways highlight its importance in cellular architecture and signaling.
- Associated diseases and disorders
Alterations in gamma Catenin expression or function relate to conditions such as heart diseases specifically arrhythmogenic right ventricular cardiomyopathy and cancer particularly affecting epithelial tissues. In these conditions abnormal gamma Catenin interactions with other junction proteins like desmoplakin and plakophilin contribute to disease progression. These disruptions suggest its potential as a therapeutic target providing insight into disease mechanisms and opportunities for intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Western blot - Anti-gamma Catenin antibody [EPR17310] - BSA and Azide free (ab238447), expandable thumbnail](https://content.abcam.com/products/images/gamma-catenin-antibody-epr17310-bsa-and-azide-free-ab238447--western-blot-img6193.jpg "Western blot - Anti-gamma Catenin antibody [EPR17310] - BSA and Azide free (ab238447)")
Western blot - Anti-gamma Catenin antibody [EPR17310] - BSA and Azide free (ab238447)
This data was developed using the same antibody clone in a different buffer formulation (Anti-gamma Catenin antibody [EPR17310] ab184919).
Lanes 1-4: Merged signal (red and green). Green - Anti-gamma Catenin antibody [EPR17310] ab184919 observed at 82 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.
Anti-gamma Catenin antibody [EPR17310] ab184919 Anti-gamma Catenin antibody [EPR17310] was shown to specifically react with gamma Catenin in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human JUP (gamma Catenin) knockout HEK-293T cell line ab266272 (knockout cell lysate Human JUP (gamma Catenin) knockout HEK-293T cell lysate ab257269) was used. Wild-type and gamma Catenin knockout samples were subjected to SDS-PAGE. Anti-gamma Catenin antibody [EPR17310] ab184919 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-gamma Catenin antibody [EPR17310] (Anti-gamma Catenin antibody [EPR17310] ab184919) at 1/1000 dilution
Lane 1: Wild-type HEK-293T cell lysate at 20 µg
Lane 2: JUZ knockout HEK-293T cell lysate at 20 µg
Lane 2: Western blot - Human JUP (gamma Catenin) knockout HEK-293T cell line (Human JUP (gamma Catenin) knockout HEK-293T cell line ab266272)
Lane 3: A431 cell lysate at 20 µg
Lane 4: THP-1 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 82 kDa
Observed band size: 82 kDa
![Flow Cytometry (Intracellular) - Anti-gamma Catenin antibody [EPR17310] - BSA and Azide free (ab238447), expandable thumbnail](https://content.abcam.com/products/images/gamma-catenin-antibody-epr17310-bsa-and-azide-free-ab238447--flow-cytometry-intracellular-img137326.jpg "Flow Cytometry (Intracellular) - Anti-gamma Catenin antibody [EPR17310] - BSA and Azide free (ab238447)")
Flow Cytometry (Intracellular) - Anti-gamma Catenin antibody [EPR17310] - BSA and Azide free (ab238447)
Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) labelling gamma Catenin with purified Anti-gamma Catenin antibody [EPR17310] ab184919 at 1/1000 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Alexa Fluor® 488 goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-gamma Catenin antibody [EPR17310] ab184919).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310] - BSA and Azide free (ab238447), expandable thumbnail](https://content.abcam.com/products/images/gamma-catenin-antibody-epr17310-bsa-and-azide-free-ab238447--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img94192.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310] - BSA and Azide free (ab238447)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310] - BSA and Azide free (ab238447)
Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling gamma Catenin with Anti-gamma Catenin antibody [EPR17310] ab184919 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Cytoplasmic and membrane staining on epithelial cells of Human prostate is observed; and smooth muscle cells are also positively stained. Counterstained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-gamma Catenin antibody [EPR17310] ab184919).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310] - BSA and Azide free (ab238447), expandable thumbnail](https://content.abcam.com/products/images/gamma-catenin-antibody-epr17310-bsa-and-azide-free-ab238447--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img93192.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310] - BSA and Azide free (ab238447)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310] - BSA and Azide free (ab238447)
Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling gamma Catenin with Anti-gamma Catenin antibody [EPR17310] ab184919 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Cytoplasmic and membrane staining on epithelial cells of Human skin is observed. Counterstained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-gamma Catenin antibody [EPR17310] ab184919).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310] - BSA and Azide free (ab238447), expandable thumbnail](https://content.abcam.com/products/images/gamma-catenin-antibody-epr17310-bsa-and-azide-free-ab238447--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img108465.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310] - BSA and Azide free (ab238447)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310] - BSA and Azide free (ab238447)
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling gamma Catenin with Anti-gamma Catenin antibody [EPR17310] ab184919 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Cytoplasmic and membrane staining on epithelial cells of mouse stomach is observed. Counterstained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-gamma Catenin antibody [EPR17310] ab184919).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310] - BSA and Azide free (ab238447), expandable thumbnail](https://content.abcam.com/products/images/gamma-catenin-antibody-epr17310-bsa-and-azide-free-ab238447--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img58524.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310] - BSA and Azide free (ab238447)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310] - BSA and Azide free (ab238447)
Immunohistochemical analysis of paraffin-embedded rat skin tissue labeling gamma Catenin with Anti-gamma Catenin antibody [EPR17310] ab184919 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Membrane and cytoplasmic staining on the epithelial cells of rat skin is observed; and smooth muscle cells are also positively stained. Counterstained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-gamma Catenin antibody [EPR17310] ab184919).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-gamma Catenin antibody [EPR17310] - BSA and Azide free (ab238447), expandable thumbnail](https://content.abcam.com/products/images/gamma-catenin-antibody-epr17310-bsa-and-azide-free-ab238447--immunocytochemistry-immunofluorescence-img68699.jpg "Immunocytochemistry/ Immunofluorescence - Anti-gamma Catenin antibody [EPR17310] - BSA and Azide free (ab238447)")
Immunocytochemistry/ Immunofluorescence - Anti-gamma Catenin antibody [EPR17310] - BSA and Azide free (ab238447)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling gamma Catenin with Anti-gamma Catenin antibody [EPR17310] ab184919 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic staining on HeLa cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: Anti-gamma Catenin antibody [EPR17310] ab184919 at 1/250 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-gamma Catenin antibody [EPR17310] ab184919).
![Immunocytochemistry/ Immunofluorescence - Anti-gamma Catenin antibody [EPR17310] - BSA and Azide free (ab238447), expandable thumbnail](https://content.abcam.com/products/images/gamma-catenin-antibody-epr17310-bsa-and-azide-free-ab238447--immunocytochemistry-immunofluorescence-img21880.jpg "Immunocytochemistry/ Immunofluorescence - Anti-gamma Catenin antibody [EPR17310] - BSA and Azide free (ab238447)")
Immunocytochemistry/ Immunofluorescence - Anti-gamma Catenin antibody [EPR17310] - BSA and Azide free (ab238447)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MDCK (Canine kidney cell line) cells labeling gamma Catenin with Anti-gamma Catenin antibody [EPR17310] ab184919 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing membranous staining on MDCK cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: Anti-gamma Catenin antibody [EPR17310] ab184919 at 1/250 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-gamma Catenin antibody [EPR17310] ab184919).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310] - BSA and Azide free (ab238447), expandable thumbnail](https://content.abcam.com/products/images/gamma-catenin-antibody-epr17310-bsa-and-azide-free-ab238447--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img58524.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310] - BSA and Azide free (ab238447)")
![Immunocytochemistry/ Immunofluorescence - Anti-gamma Catenin antibody [EPR17310] - BSA and Azide free (ab238447), expandable thumbnail](https://content.abcam.com/products/images/gamma-catenin-antibody-epr17310-bsa-and-azide-free-ab238447--immunocytochemistry-immunofluorescence-img68699.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-gamma Catenin antibody [EPR17310] - BSA and Azide free (ab238447)")
![Immunocytochemistry/ Immunofluorescence - Anti-gamma Catenin antibody [EPR17310] - BSA and Azide free (ab238447), expandable thumbnail](https://content.abcam.com/products/images/gamma-catenin-antibody-epr17310-bsa-and-azide-free-ab238447--immunocytochemistry-immunofluorescence-img21880.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-gamma Catenin antibody [EPR17310] - BSA and Azide free (ab238447)")
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