AB29041

Anti-gamma Catenin (phospho Y550) antibody

Name: Anti-gamma Catenin (phospho Y550) antibody (ab29041) | Abcam
Brand: Abcam Limited
SKU: AB29041

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(1 Publication)

Rabbit Polyclonal gamma Catenin phospho Y550 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human JUP phospho Y550 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CTNNG, DP3, JUP, Junction plakoglobin, Catenin gamma, Desmoplakin III, Desmoplakin-3

1 Images

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Western blot - Anti-gamma Catenin (phospho Y550) antibody (AB29041)

Western blot analysis of gamma-Catenin immunoprecipitated from lysates prepared from A431 cells treated with pervanadate. The immunoprecipitation was performed using mouse monoclonal anti-gamma-Catenin. The blots were probed with anti-phospho-gamma-Catenin (Tyr-550) antibody. The immunoprecipitated gamma-Catenin was untreated (lane 1) or treated with alkaline phosphatase (lane 2) then probed with the antibody. In addition, the anti-phospho-gamma-Catenin (Tyr-550) was used in the presence of phospho-gamma-Catenin (Tyr-550) peptide (lane 3) or phospho-gamma-Catenin (Tyr-644) peptide (lane 4).

All lanes:

Western blot - Anti-gamma Catenin (phospho Y550) antibody (ab29041)

Predicted band size: 82 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human JUP phospho Y550 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P14923

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p>In 5% non fat milk, PBS, 0.04% Tween 20 for 1hour at room temperature.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab29041 was cross adsorbed to both phospho tyrosine coupled to agarose and to dephosphorylated gamma Catenin (Tyr550) peptide before immunogen affinity purification (without carrier protein).

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Gamma Catenin also known as Junction Plakoglobin (JUP) is a protein with a molecular mass of approximately 82 kDa. It plays an important role in cell adhesion mechanisms. This protein is expressed widely in various tissue types prominently in epithelial tissues. Gamma Catenin contributes significantly to the structure of adherens junctions and desmosomes physically connecting cells to one another and stabilizing those connections within tissues.

Biological function summary

Gamma Catenin is an integral component of the armadillo family of proteins and part of desmosomal and adherens junction complexes. It plays an essential role in maintaining the integrity and mechanical strength of tissues. Through interacting with other proteins in these complexes gamma Catenin facilitates cell signaling and communication. It associates with cadherins and catenins including plakophilin and β-catenin to ensure proper cell-cell adhesion and signaling across the cell membrane.

Pathways

Gamma Catenin is involved in the Wnt signaling pathway an important route governing cellular processes such as proliferation differentiation and migration. Gamma Catenin interacts with β-catenin in this pathway influencing transcriptional regulation in the nucleus. It also plays roles in the cadherin-mediated cell adhesion pathway working alongside other proteins like E-cadherin to modulate cell behavior. These pathways highlight its importance in cellular architecture and signaling.

Alterations in gamma Catenin expression or function relate to conditions such as heart diseases specifically arrhythmogenic right ventricular cardiomyopathy and cancer particularly affecting epithelial tissues. In these conditions abnormal gamma Catenin interactions with other junction proteins like desmoplakin and plakophilin contribute to disease progression. These disruptions suggest its potential as a therapeutic target providing insight into disease mechanisms and opportunities for intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE-cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton (By similarity).

See full target information JUP phospho Y550

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Leukemia 27:336-43 PubMed22858986

2012

γ-Catenin is overexpressed in acute myeloid leukemia and promotes the stabilization and nuclear localization of β-catenin.

Applications

Unspecified application

Species

Unspecified reactive species

R G Morgan,L Pearn,K Liddiard,S L Pumford,A K Burnett,A Tonks,R L Darley

View all publications

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Product promise

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Anti-gamma Catenin (phospho Y550) antibody

Western blot - Anti-gamma Catenin (phospho Y550) antibody (AB29041)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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