Rabbit Polyclonal gamma Catenin phospho Y550 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human JUP phospho Y550 conjugated to Keyhole Limpet Haemocyanin.
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Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human JUP phospho Y550 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P14923
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes In 5% non fat milk, PBS, 0.04% Tween 20 for 1hour at room temperature. |
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Target data
Function
Common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE-cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton (By similarity).
Alternative names
CTNNG, DP3, JUP, Junction plakoglobin, Catenin gamma, Desmoplakin III, Desmoplakin-3
Recommended products
Rabbit Polyclonal gamma Catenin phospho Y550 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human JUP phospho Y550 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human JUP phospho Y550 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P14923
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab29041 was cross adsorbed to both phospho tyrosine coupled to agarose and to dephosphorylated gamma Catenin (Tyr550) peptide before immunogen affinity purification (without carrier protein).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Gamma Catenin also known as Junction Plakoglobin (JUP) is a protein with a molecular mass of approximately 82 kDa. It plays an important role in cell adhesion mechanisms. This protein is expressed widely in various tissue types prominently in epithelial tissues. Gamma Catenin contributes significantly to the structure of adherens junctions and desmosomes physically connecting cells to one another and stabilizing those connections within tissues.
- Biological function summary
Gamma Catenin is an integral component of the armadillo family of proteins and part of desmosomal and adherens junction complexes. It plays an essential role in maintaining the integrity and mechanical strength of tissues. Through interacting with other proteins in these complexes gamma Catenin facilitates cell signaling and communication. It associates with cadherins and catenins including plakophilin and β-catenin to ensure proper cell-cell adhesion and signaling across the cell membrane.
- Pathways
Gamma Catenin is involved in the Wnt signaling pathway an important route governing cellular processes such as proliferation differentiation and migration. Gamma Catenin interacts with β-catenin in this pathway influencing transcriptional regulation in the nucleus. It also plays roles in the cadherin-mediated cell adhesion pathway working alongside other proteins like E-cadherin to modulate cell behavior. These pathways highlight its importance in cellular architecture and signaling.
- Associated diseases and disorders
Alterations in gamma Catenin expression or function relate to conditions such as heart diseases specifically arrhythmogenic right ventricular cardiomyopathy and cancer particularly affecting epithelial tissues. In these conditions abnormal gamma Catenin interactions with other junction proteins like desmoplakin and plakophilin contribute to disease progression. These disruptions suggest its potential as a therapeutic target providing insight into disease mechanisms and opportunities for intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-gamma Catenin (phospho Y550) antibody (ab29041)
Western blot analysis of gamma-Catenin immunoprecipitated from lysates prepared from A431 cells treated with pervanadate. The immunoprecipitation was performed using mouse monoclonal anti-gamma-Catenin. The blots were probed with anti-phospho-gamma-Catenin (Tyr-550) antibody. The immunoprecipitated gamma-Catenin was untreated (lane 1) or treated with alkaline phosphatase (lane 2) then probed with the antibody. In addition, the anti-phospho-gamma-Catenin (Tyr-550) was used in the presence of phospho-gamma-Catenin (Tyr-550) peptide (lane 3) or phospho-gamma-Catenin (Tyr-644) peptide (lane 4).
All lanes: Western blot - Anti-gamma Catenin (phospho Y550) antibody (ab29041)
Predicted band size: 82 kDa
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