AB11174

Anti-gamma H2A.X (phospho S139) antibody

Name: Anti-gamma H2A.X (phospho S139) antibody (ab11174) | Abcam
Brand: Abcam Limited
SKU: AB11174
Price: 400 USD
Availability: InStock
Rating: 5 (28 reviews)

(28 Reviews)

(386 Publications)

Anti-gamma H2A.X (phospho S139) antibody (ab11174) is a rabbit polyclonal antibody detecting gamma H2A.X in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse.

- Over 300 publications
- Trusted since 2004

View Alternative Names

H2AFX, H2AX, Histone H2AX, H2a/x, Histone H2A.X, H2AS139p, H2AXS139p, H2A.XS139p

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma H2A.X (phospho S139) antibody (AB11174)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma H2A.X (phospho S139) antibody (AB11174)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labeling gamma H2A.X (phospho S139) with ab11174 at 1/5000 dilution. Heat mediated antigen retrieval was performed using citrate buffer pH 6.

Immunocytochemistry/ Immunofluorescence - Anti-gamma H2A.X (phospho S139) antibody (AB11174)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-gamma H2A.X (phospho S139) antibody (AB11174)

Immunocytochemistry/Immunofluorescence analysis of neocarzinostatin treated asynchronous HeLa cells (left) and untreated asynchronous HeLa cells (right) labelling H2A.X (phospho S139 with ab11174 at 1/5000 (0.2µg/ml). A DyLight® 594-conjugated anti-rabbit IgG (1/100) was used as the secondary antibody.

ICC/IF

AbReview7135****

Immunocytochemistry/ Immunofluorescence - Anti-gamma H2A.X (phospho S139) antibody (AB11174)

ab11174 at 1/1000 staining human HeLa cells by ICC/IF. These cells express a gene that causes a DNA damage response, leading to H2AX phosphorylation. The cells were paraformaldehyde fixed and blocked with BSA prior to incubation with the antibody for 45 minutes. An Alexa-Fluor ® 488 conjugated goat anti-rabbit was used as the secondary.

This image is courtesy of an anonymous Abreview

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-gamma H2A.X (phospho S139) antibody (AB11174)

ab11174 staining gamma H2A.X (phospho S139) in HeLa UV cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab11174 at 0.1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor^® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma H2A.X (phospho S139) antibody (AB11174)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse CT26 colon carcinoma tissue labeling gamma H2A.X (phospho S139) with ab11174 at 1/5000 dilution. Heat mediated antigen retrieval was performed using citrate buffer pH 6.

Supplier Data

Western blot - Anti-gamma H2A.X (phospho S139) antibody (AB11174)

Lower Panel shows western blot for total H2AX using rabbit anti-H2AX recombinant monoclonal antibody.

All lanes:

Western blot - Anti-gamma H2A.X (phospho S139) antibody (ab11174) at 0.04 µg/mL

Lane 1:

Jurkat (human T cell leukemia cell line from peripheral blood) cells treated with 100 µM etoposide, whole cell lysate at 50 µg

Lane 2:

Jurkat cells mock treated, whole cell lysate at 50 µg

Secondary

All lanes:

Goat anti-rabbit IgG (HRP)

Predicted band size: 15 kDa

false

Exposure time: 10s

AbReview41562****

Western blot - Anti-gamma H2A.X (phospho S139) antibody (AB11174)

All lanes:

Western blot - Anti-gamma H2A.X (phospho S139) antibody (ab11174) at 1/5000 dilution

Lane 1:

HeLa nuclear lysate - untreated at 40 µg

Lane 2:

HeLa nuclear lysate - IR treated at 40 µg

Secondary

All lanes:

HRP-conjugated donkey anti-rabbit IgG polyclonal

Predicted band size: 15 kDa

Observed band size: 17 kDa

true

Exposure time: 30s

This image is courtesy of an anonymous Abreview

Supplier Data

Western blot - Anti-gamma H2A.X (phospho S139) antibody (AB11174)

Lower Panel : Rabbit anti-GAPDH antibody.

All lanes:

Western blot - Anti-gamma H2A.X (phospho S139) antibody (ab11174) at 0.04 µg/mL

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) cells treated with 100 µM etoposide, whole cell lysate at 50 µg

Lane 2:

NIH/3T3 cells mock treated, whole cell lysate at 50 µg

Secondary

All lanes:

Goat anti-rabbit IgG (HRP)

Predicted band size: 15 kDa

false

Exposure time: 3s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human H2AX phospho S139. The exact immunogen used to generate this antibody is proprietary information.

P16104

Specificity

Using IF, this antibody was shown to bind to a non-nuclear location in Hela cells.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/5000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/5000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/5000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/5000", "ICCIF-species-notes": "" }, "Bat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chinese hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Guinea pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Anti-gamma H2A.X (phospho S139) antibody (ab11174) is a rabbit polyclonal antibody and is validated for use in ICC/IF, IHC-P, WB in human, mouse, rat samples.

Anti-gamma H2A.X (phospho S139) antibody (ab11174) has been cited over 303 times in peer reviewed journals and is trusted by the scientific community.

Abcam's high quality validation processes ensure Anti-gamma H2A.X (phospho S139) antibody (ab11174) has high sensitivity and specificity.

Anti-gamma H2A.X (phospho S139) antibody (ab11174) has 26 independent reviews from customers.

Anti-gamma H2A.X (phospho S139) antibody (ab11174) specifically detects gamma H2A.X Phospho-S139 (UniProt ID: P16104; Molecular weight: 15kDa) and is sold in 50 µg selling sizes.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antibodies were affinity purified using the peptide immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Gamma H2A.X also known as phospho H2A.X or γH2A.X is a phosphorylated form of the histone variant H2A.X. It has a molecular weight of about 14 kilodaltons and occurs primarily in they nucleus. When DNA double-strand breaks (DSBs) occur serine 139 in H2A.X undergoes rapid phosphorylation resulting in gamma H2A.X. This modification happens swiftly at the site of damage and gamma H2A.X spreads over a large chromatin area facilitating the recruitment of DNA repair proteins. Gamma H2A.X staining typically evaluated using gamma H2A.X immunofluorescence techniques aids in identifying the presence and extent of DNA damage.

Biological function summary

Gamma H2A.X plays a role in DNA damage response and repair. It does not operate alone; it acts as part of a complex with other repair proteins. The formation of gamma H2A.X foci at DNA damage sites creates a signal attracting repair factors that help maintain genome stability. Its interaction with MDC1 and ATM proteins exemplifies its significant role in orchestrating an effective response to DNA damage. Beyond DNA repair gamma H2A.X influences cell cycle checkpoints permitting cells to pause and repair before proceeding with division.

Pathways

Gamma H2A.X plays a pivotal role in the DNA damage response (DDR) pathway. This pathway is essential for detecting and repairing DNA lesions to uphold genomic integrity. Within the DDR pathway gamma H2A.X is closely associated with proteins such as NBS1 and BRCA1 which assist in repairing double-strand breaks. In addition gamma H2A.X is integral to the ATM-ATR signaling pathway where its activation promotes cell survival following genotoxic stress by signaling for damage repair or triggering apoptosis.

Gamma H2A.X has connections to cancer and neurodegeneration. Aberrant DNA repair pathways often indicated by persistent gamma H2A.X signals correlate with tumor formation and progression. For instance a failure to repair DNA damage effectively can lead to mutations that drive cancer development. Gamma H2A.X also links to neurodegenerative diseases where dysregulated DNA repair contributes to neuronal cell death. Proteins like p53 which regulate cell cycle and apoptosis further connect to gamma H2A.X bridging its role in disease pathogenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by the H2AX gene is a variant histone H2A that replaces conventional H2A in certain nucleosomes, which are responsible for wrapping and compacting DNA into chromatin. This compaction limits DNA accessibility to cellular machineries that require DNA as a template, placing histones at the center of transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is controlled through a complex array of post-translational histone modifications, known as the histone code, and nucleosome remodeling. The H2AX protein is essential for the checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for the efficient repair of DNA double strand breaks (DSBs), particularly when it undergoes C-terminal phosphorylation. This supplementary information is collated from multiple sources and compiled automatically.

See full target information H2AX phospho S139

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Publications (386)

Recent publications for all applications. Explore the full list and refine your search

Clinical and translational radiation oncology 56:101050 PubMed41049393

2025

No differences in therapeutic efficacy while sparing healthy tissue for orthotopic glioblastoma patient-derived xenografts in context of proton FLASH.

Applications

Unspecified application

Species

Unspecified reactive species

Taylor L Schanel,Manoj Kumar,Lauren C Nassour-Caswell,Sreelakshmi Cherakara,Rhea Pandit,Andee M Beierle,Joshua C Anderson,Patrica H Hicks,Rex Cardan,Anita B Hjelmeland,Christopher D Willey

The Journal of clinical investigation 135: PubMed40955658

2025

CDK12/13 inactivation triggers STING-mediated antitumor immunity in preclinical models.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Bao,Yu Chang,Jean Ching-Yi Tien,Gabriel Cruz,Fan Yang,Rahul Mannan,Somnath Mahapatra,Radha Paturu,Xuhong Cao,Fengyun Su,Rui Wang,Yuping Zhang,Mahnoor Gondal,Jae Eun Choi,Jonathan K Gurkan,Stephanie J Miner,Dan R Robinson,Yi-Mi Wu,Licheng Zhou,Zhen Wang,Ilona Kryczek,Xiaoju Wang,Marcin Cieslik,Yuanyuan Qiao,Alexander Tsodikov,Weiping Zou,Ke Ding,Arul M Chinnaiyan

OncoTargets and therapy 18:979-994 PubMed40934061

2025

The Dual Role of RBBP7 in Esophageal Squamous Cell Carcinoma: Cell Context-Dependent Impacts on Proliferation and Radiosensitivity.

Applications

Unspecified application

Species

Unspecified reactive species

Yafen Li,Shuai Lu,Hui Yao,Genbao Zhu,Hao Liu,Zhiyu Ma,Heng Tang

Zoological research 46:1079-1092 PubMed40923305

2025

Testis-specific protein HSF5 is essential for proper chromatin organization and transcriptional reprogramming to drive pachynema progression.

Applications

Unspecified application

Species

Unspecified reactive species

Chun-Hai Luo,Zhi-Wei Fan,Zi-Qi Yu,Da-Lin Liu,Hao-Ran Xu,Shu-Min Zhou,Xuan-Jing Zhu,Han-Chao Liu,Li-Fu Shao,Zhe-An Li,Chong Xie,Jun-Feng Zhan,Fei Sun

Nature aging 5:1844-1861 PubMed40913219

2025

A blood-based DNA damage signature in patients with Parkinson's disease is associated with disease progression.

Applications

Unspecified application

Species

Unspecified reactive species

Daisy Sproviero,César Payán-Gómez,Chiara Milanese,Sander Barnhoorn,Shixiang Sun,Akos Gyenis,Domenico Delia,Tammaryn Lashley,Jan H J Hoeijmakers,Jan Vijg,Pier G Mastroberardino

PLoS pathogens 21:e1013142 PubMed40825038

2025

Adeno-Associated Virus 2 (AAV2) - Induced RPA exhaustion generates cellular DNA damage and restricts viral gene expression.

Applications

Unspecified application

Species

Unspecified reactive species

Monnette F Summers,MegAnn K Haubold,Marcel Morgenstern,Phoenix Shepherd,Clairine I S Larsen,Ava E Bartz,Gopishankar Thirumoorthy,Robert N Kirchdoerfer,Joshua J Coon,Kavi P M Mehta,Kinjal Majumder

iScience 28:113119 PubMed40822342

2025

DAXX-dependent H3.3 deposition maintains myoblast cell identity independently of other histone chaperone complexes.

Applications

Unspecified application

Species

Unspecified reactive species

Virginia Zoglio,Sarah Chebouti,Fayez Issa,Frédéric Relaix,Joana Esteves de Lima

Nature communications 16:7064 PubMed40750587

2025

Tumor control and immune activation through palliative irradiation and ATR inhibition, PATRIOT Part C: a phase Ib trial.

Applications

Unspecified application

Species

Unspecified reactive species

Magnus T Dillon,Emmanuel C Patin,Kabir Mohammed,Jeane Guevara,Simon A Smith,Emma Dean,Heba Soliman,Pablo Nenclares,Motoko Ryugenji,Davina Northcote,Neel Shah,Lorna Grove,Christopher J Lord,Stephen Pettit,Matt Tall,Karen E Swales,Udai Banerji,Alan A Melcher,Mark Saunders,Martin D Forster,Kevin J Harrington

Oncogenesis 14:20 PubMed40533448

2025

ONC201 enhances the cytotoxic effect of cisplatin through ATF3/ATF4/CHOP in head and neck squamous cell carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hui-Ching Chuang,Ming-Hsien Tsai,Jiin-Haur Chuang,Ya-Ting Hong,Chih-Yen Chien,Ming-Huei Chou

iScience 28:112637 PubMed40520087

2025

The Arg/N-degron pathway mediates the secretion of apoptotic exosomes under oxidative stress in cancer cell.

Applications

Unspecified application

Species

Unspecified reactive species

Su Bin Kim,Ji Su Lee,Chan Hoon Jung,Eun Hye Cho,Ho Seok Seo,Gee Eun Lee,Hye Yeon Kim,Su Jin Lee,Min Ju Lee,Hans Jin-Young Oh,Ah Jung Heo,Do Hyun Han,Yong Tae Kwon,Chang Hoon Ji

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-gamma H2A.X (phospho S139) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma H2A.X (phospho S139) antibody (AB11174)

Immunocytochemistry/ Immunofluorescence - Anti-gamma H2A.X (phospho S139) antibody (AB11174)

Immunocytochemistry/ Immunofluorescence - Anti-gamma H2A.X (phospho S139) antibody (AB11174)

Immunocytochemistry/ Immunofluorescence - Anti-gamma H2A.X (phospho S139) antibody (AB11174)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma H2A.X (phospho S139) antibody (AB11174)

Western blot - Anti-gamma H2A.X (phospho S139) antibody (AB11174)

Western blot - Anti-gamma H2A.X (phospho S139) antibody (AB11174)

Western blot - Anti-gamma H2A.X (phospho S139) antibody (AB11174)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (386)

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