AB228655

Anti-gamma H2A.X (phospho S139) antibody - C-terminal

Name: Anti-gamma H2A.X (phospho S139) antibody - C-terminal (ab228655) | Abcam
Brand: Abcam Limited
SKU: AB228655

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(6 Publications)

Rabbit Polyclonal H2A.X phospho S139 antibody. C-terminal. Suitable for WB, IHC - Wmt and reacts with Zebrafish samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Zebrafish h2ax pS139.

View Alternative Names

zgc:56329, h2ax, Histone H2AX, H2a/x, Histone H2A.X, H2AS139p, H2AXS139p, H2A.XS139p

1 Images

IHC - Wholemount - Anti-gamma H2A.X (phospho S139) antibody - C-terminal (AB228655)

IHC - Wmt

Supplier Data

IHC - Wholemount - Anti-gamma H2A.X (phospho S139) antibody - C-terminal (AB228655)

24 hours-post-fertilization, UV-irradiated zebrafish embryo stained for Histone H2A.X (phospho S139) with ab228655 (green) at 1/100 dilution in immunohistochemical wholemount analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Zebrafish

Applications

WB, IHC - Wmt

applications

Immunogen

Synthetic Peptide within Zebrafish h2ax pS139. The exact immunogen used to generate this antibody is proprietary information.

Q7ZUY3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCWmt" : {"fullname" : "IHC - Wholemount", "shortname":"IHC - Wmt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Zebrafish": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCWmt-species-checked": "testedAndGuaranteed", "IHCWmt-species-dilution-info": "1/100 - 1/500", "IHCWmt-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Gamma H2A.X also known as phospho H2A.X or γH2A.X is a phosphorylated form of the histone variant H2A.X. It has a molecular weight of about 14 kilodaltons and occurs primarily in they nucleus. When DNA double-strand breaks (DSBs) occur serine 139 in H2A.X undergoes rapid phosphorylation resulting in gamma H2A.X. This modification happens swiftly at the site of damage and gamma H2A.X spreads over a large chromatin area facilitating the recruitment of DNA repair proteins. Gamma H2A.X staining typically evaluated using gamma H2A.X immunofluorescence techniques aids in identifying the presence and extent of DNA damage.

Biological function summary

Gamma H2A.X plays a role in DNA damage response and repair. It does not operate alone; it acts as part of a complex with other repair proteins. The formation of gamma H2A.X foci at DNA damage sites creates a signal attracting repair factors that help maintain genome stability. Its interaction with MDC1 and ATM proteins exemplifies its significant role in orchestrating an effective response to DNA damage. Beyond DNA repair gamma H2A.X influences cell cycle checkpoints permitting cells to pause and repair before proceeding with division.

Pathways

Gamma H2A.X plays a pivotal role in the DNA damage response (DDR) pathway. This pathway is essential for detecting and repairing DNA lesions to uphold genomic integrity. Within the DDR pathway gamma H2A.X is closely associated with proteins such as NBS1 and BRCA1 which assist in repairing double-strand breaks. In addition gamma H2A.X is integral to the ATM-ATR signaling pathway where its activation promotes cell survival following genotoxic stress by signaling for damage repair or triggering apoptosis.

Gamma H2A.X has connections to cancer and neurodegeneration. Aberrant DNA repair pathways often indicated by persistent gamma H2A.X signals correlate with tumor formation and progression. For instance a failure to repair DNA damage effectively can lead to mutations that drive cancer development. Gamma H2A.X also links to neurodegenerative diseases where dysregulated DNA repair contributes to neuronal cell death. Proteins like p53 which regulate cell cycle and apoptosis further connect to gamma H2A.X bridging its role in disease pathogenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation (By similarity).

See full target information h2ax pS139

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Cancers 15: PubMed37190291

2023

Repurposing Sulfasalazine as a Radiosensitizer in Hypoxic Human Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lisa Kerkhove,Febe Geirnaert,Amir Laraki Rifi,Ka Lun Law,Adrián Gutiérrez,Inge Oudaert,Cyril Corbet,Thierry Gevaert,Inès Dufait,Mark De Ridder

International journal of molecular sciences 23: PubMed36232766

2022

Understanding the Function and Mechanism of Zebrafish Tmem39b in Regulating Cold Resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Renyan Liu,Yong Long,Ran Liu,Guili Song,Qing Li,Huawei Yan,Zongbin Cui

Cell death discovery 8:246 PubMed35508610

2022

Anti-fibrotic mechanism of SPP1 knockdown in atrial fibrosis associates with inhibited mitochondrial DNA damage and TGF-β/SREBP2/PCSK9 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xianfeng Du,Ting Liu,Caijie Shen,Bin He,Mingjun Feng,Jing Liu,Weidong Zhuo,Guohua Fu,Binhao Wang,Yanyan Xu,Huimin Chu

Molecular neurodegeneration 16:69 PubMed34593012

2021

The TGFβ/Notch axis facilitates Müller cell-to-epithelial transition to ultimately form a chronic glial scar.

Applications

Unspecified application

Species

Unspecified reactive species

Federica Maria Conedera,Ana Maria Quintela Pousa,Nadia Mercader,Markus Tschopp,Volker Enzmann

American journal of physiology. Regulatory, integr 318:R843-R854 PubMed32186196

2020

Cisplatin nephrotoxicity is induced via poly(ADP-ribose) polymerase activation in adult zebrafish and mice.

Applications

Unspecified application

Species

Unspecified reactive species

Myoung-Jin Kim,Daeun Moon,Sumi Jung,Jehee Lee,Jinu Kim

Glia 67:1150-1166 PubMed30794326

2019

Retinal microglia signaling affects Müller cell behavior in the zebrafish following laser injury induction.

Applications

Unspecified application

Species

Unspecified reactive species

Federica Maria Conedera,Ana Maria Quintela Pousa,Nadia Mercader,Markus Tschopp,Volker Enzmann

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-gamma H2A.X (phospho S139) antibody - C-terminal

IHC - Wholemount - Anti-gamma H2A.X (phospho S139) antibody - C-terminal (AB228655)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise