AB104478

Anti-gamma Sarcoglycan antibody

Name: Anti-gamma Sarcoglycan antibody (ab104478) | Abcam
Brand: Abcam Limited
SKU: AB104478
Rating: 3 (1 reviews)

(1 Review)

(1 Publication)

Rabbit Polyclonal gamma Sarcoglycan antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SGCG.

View Alternative Names

Gamma-sarcoglycan, Gamma-SG, 35 kDa dystrophin-associated glycoprotein, 35DAG, SGCG

2 Images

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Western blot - Anti-gamma Sarcoglycan antibody (AB104478)

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Western blot - Anti-gamma Sarcoglycan antibody (ab104478) at 1 µg/mL

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gamma Sarcoglycan transfected 293T cell lysate at 25 µg

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Non-transfected 293T cell lysate at 25 µg

Predicted band size: 32 kDa

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Western blot - Anti-gamma Sarcoglycan antibody (AB104478)

All lanes:

Western blot - Anti-gamma Sarcoglycan antibody (ab104478) at 1 µg/mL

All lanes:

Human kidney tissue lysate at 50 µg

Predicted band size: 32 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

Recombinant Full Length Protein corresponding to Human SGCG.

Q13326

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Gamma sarcoglycan also known as γ-sarcoglycan is a transmembrane protein with a molecular mass of approximately 35-50 kDa. It plays a mechanical role by stabilizing muscle cell membranes. Filamentous and inelastic it integrates into the dystrophin-associated glycoprotein complex (DGC) within skeletal and cardiac muscles. Cells express gamma sarcoglycan primarily in muscle tissue making it vital for muscle integrity and function.

Biological function summary

Gamma sarcoglycan acts as a component of the sarcoglycan complex part of the larger dystrophin-glycoprotein complex. This complex connects the cytoskeleton of a muscle fiber to the extracellular matrix maintaining structural stability under mechanical stress. Its role is to ensure force transmission while preventing membrane damage along with proteins like dystrophin beta sarcoglycan and delta sarcoglycan. Gamma sarcoglycan's expression is important for proper muscle contraction and resilience.

Pathways

Gamma sarcoglycan associates with the dystrophin-glycoprotein complex pathway contributing to cellular stability and muscle fiber integrity. It also intersects with the actin cytoskeleton signaling pathway where it modulates structural organization. In these pathways it works alongside dystrophin to secure muscle fibers during contraction and relaxation cycles preventing damage from mechanical strain.

Mutations in the gamma sarcoglycan gene lead to limb-girdle muscular dystrophy type 2C (LGMD2C) characterized by progressive muscle weakness. This genetic disorder causes instability in the sarcoglycan complex resulting in muscle fiber degradation. In another condition dilated cardiomyopathy defects in gamma sarcoglycan disrupt heart muscle function. These diseases often involve interactions with proteins like dystrophin and other sarcoglycans which also contribute to muscle deterioration.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.

See full target information SGCG

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of neurology, neurosurgery, and psychiatry 85:92-8 PubMed23667215

2013

Clinical characterisation of Becker muscular dystrophy patients predicts favourable outcome in exon-skipping therapy.

Applications

Unspecified application

Species

Human

J C van den Bergen,S M Schade van Westrum,L Dekker,A J van der Kooi,M de Visser,B H A Wokke,C S Straathof,M A Hulsker,A Aartsma-Rus,J J Verschuuren,H B Ginjaar

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