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AB251689

Anti-gamma Sarcoglycan antibody

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(1 Publication)

Rabbit Polyclonal gamma Sarcoglycan antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SGCG aa 50-200.

View Alternative Names

Gamma-sarcoglycan, Gamma-SG, 35 kDa dystrophin-associated glycoprotein, 35DAG, SGCG

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody (AB251689)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody (AB251689)

Paraffin-embedded human heart muscle tissue stained for gamma Sarcoglycan using ab251689 at 1/20 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody (AB251689)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody (AB251689)

Paraffin-embedded human prostate tissue stained for gamma Sarcoglycan using ab251689 at 1/20 dilution in immunohistochemical analysis. Low expression as expected.

Immunocytochemistry/ Immunofluorescence - Anti-gamma Sarcoglycan antibody (AB251689)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-gamma Sarcoglycan antibody (AB251689)

PFA-fixed, Triton X-100 permeabilized U-251 MG (formally U-373 MG) (human brain glioma cell line) cells stained for gamma Sarcoglycan (green) using ab251689 at 4 µg/ml in ICC/IF.

Western blot - Anti-gamma Sarcoglycan antibody (AB251689)
  • WB

Unknown

Western blot - Anti-gamma Sarcoglycan antibody (AB251689)

All lanes:

Western blot - Anti-gamma Sarcoglycan antibody (ab251689) at 0.4 µg/mL

Lane 1:

Vector only transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) lysate

Lane 2:

gamma Sarcoglycan overexpression HEK-293T cell lysate

Predicted band size: 32 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SGCG aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

Q13326

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Gamma sarcoglycan also known as γ-sarcoglycan is a transmembrane protein with a molecular mass of approximately 35-50 kDa. It plays a mechanical role by stabilizing muscle cell membranes. Filamentous and inelastic it integrates into the dystrophin-associated glycoprotein complex (DGC) within skeletal and cardiac muscles. Cells express gamma sarcoglycan primarily in muscle tissue making it vital for muscle integrity and function.
Biological function summary

Gamma sarcoglycan acts as a component of the sarcoglycan complex part of the larger dystrophin-glycoprotein complex. This complex connects the cytoskeleton of a muscle fiber to the extracellular matrix maintaining structural stability under mechanical stress. Its role is to ensure force transmission while preventing membrane damage along with proteins like dystrophin beta sarcoglycan and delta sarcoglycan. Gamma sarcoglycan's expression is important for proper muscle contraction and resilience.

Pathways

Gamma sarcoglycan associates with the dystrophin-glycoprotein complex pathway contributing to cellular stability and muscle fiber integrity. It also intersects with the actin cytoskeleton signaling pathway where it modulates structural organization. In these pathways it works alongside dystrophin to secure muscle fibers during contraction and relaxation cycles preventing damage from mechanical strain.

Mutations in the gamma sarcoglycan gene lead to limb-girdle muscular dystrophy type 2C (LGMD2C) characterized by progressive muscle weakness. This genetic disorder causes instability in the sarcoglycan complex resulting in muscle fiber degradation. In another condition dilated cardiomyopathy defects in gamma sarcoglycan disrupt heart muscle function. These diseases often involve interactions with proteins like dystrophin and other sarcoglycans which also contribute to muscle deterioration.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.
See full target information SGCG
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Thoracic cancer 12:372-381 PubMed33350095

2020

Exosomal miR-3180-3p inhibits proliferation and metastasis of non-small cell lung cancer by downregulating FOXP4.

Applications

Unspecified application

Species

Unspecified reactive species

Tengfei Chen,Yali Liu,Jun Chen,Huifei Zheng,Qiuyun Chen,Jun Zhao
View all publications
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