Anti-gamma Sarcoglycan antibody [EPR17862-40] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody [EPR17862-40] - BSA and Azide free (AB240365)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling gamma Sarcoglycan with ab203112 at 1/500 dilution followed by ab97051 Goat Anti-Rabbit IgG H&L (HRP) at a 1/500 dilution. Cell membrane and weakly cytoplasmic staining on Human skeletal muscle tissue is observed. Counter stained with Hematoxylin.

Negative control : Used PBS instead of primary antibody, secondary antibody is ab97051 Goat Anti-Rabbit IgG H&L (HRP).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203112).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody [EPR17862-40] - BSA and Azide free (AB240365)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling gamma Sarcoglycan with ab203112 at 1/500 dilution followed by ab97051 Goat Anti-Rabbit IgG H&L (HRP) at a 1/500 dilution. Counter stained with Hematoxylin. No staining on Human colon tissue is observed.

Negative control : Used PBS instead of primary antibody, secondary antibody is ab97051 Goat Anti-Rabbit IgG H&L (HRP).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203112).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IP

Supplier Data

Immunoprecipitation - Anti-gamma Sarcoglycan antibody [EPR17862-40] - BSA and Azide free (AB240365)

gamma Sarcoglycan was immunoprecipitated from 1mg of Human fetal kidney whole cell lysate with ab203112 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab203112 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : Human fetal kidney whole cell lysate10 µg (Input). Lane 2 : ab203112 IP in Human fetal kidney whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab203112 in Human fetal kidney whole cell lysate.
Exposure time : 3 seconds

Blocking/Dilution buffer 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203112).

All lanes:

Immunoprecipitation - Anti-gamma Sarcoglycan antibody [EPR17862-40] (<a href='/en-us/products/primary-antibodies/gamma-sarcoglycan-antibody-epr17862-40-ab203112'>ab203112</a>)

Predicted band size: 32 kDa

false