AB203113

Anti-gamma Sarcoglycan antibody [EPR17862-7]

RabMAb
Recombinant
What is this?

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(2 Publications)

Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113) is a rabbit monoclonal antibody detecting gamma Sarcoglycan in Western Blot, IP, IHC-P. Suitable for Human, Mouse.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

Gamma-sarcoglycan, Gamma-SG, 35 kDa dystrophin-associated glycoprotein, 35DAG, SGCG

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody [EPR17862-7] (AB203113)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody [EPR17862-7] (AB203113)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling gamma Sarcoglycan with ab203113 at 1/500 dilution followed by ab97051 Goat Anti-Rabbit IgG H&L (HRP) at a 1/500 dilution. Cell membrane and weak cytoplasmic staining observed. Counter stained with Hematoxylin.

Negative control : Used PBS instead of primary antibody with secondary ab97051 Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody [EPR17862-7] (AB203113)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling gamma Sarcoglycan with ab203113 at 1/500 dilution followed by ab97051 Goat Anti-Rabbit IgG H&L (HRP) at a 1/500 dilution. No staining is observed on Human colon tissue. Counter stained with Hematoxylin.

Negative control : Used PBS instead of primary antibody with secondary ab97051 Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (AB203113)

Blocking/Dilution buffer

5% NFDM/TBST.

This protein is expressed in skeletal and heart muscle.

All lanes:

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113) at 1/10000 dilution

All lanes:

Human skeletal muscle whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 32 kDa

Observed band size: 32 kDa

false

Exposure time: 15s

Unknown

Immunoprecipitation - Anti-gamma Sarcoglycan antibody [EPR17862-7] (AB203113)

Human gamma Sarcoglycan was immunoprecipitated from 1mg of Human fetal kidney whole cell lysate with ab203113 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab203113 at 1/10000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1 : Human fetal kidney whole cell lysate 10 μg (Input). Lane 2 : ab203113 IP in Human fetal kidney whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab203113 in Human fetal kidney whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 3 Seconds

Blocking/Dilution buffer 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113)

Predicted band size: 32 kDa

false

Supplier Data

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (AB203113)

Blocking/dilution buffer : 5% NFDM/TBST.

This protein is expressed in skeletal and heart muscle.

ab181602 Anti-GAPDH antibody [EPR16891] control used.

All lanes:

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113) at 1/2000 dilution

Lane 1:

Human fetal brain whole tissue lysate at 10 µg

Lane 2:

Human fetal heart whole tissue lysate at 10 µg

Lane 3:

Human fetal kidney whole tissue lysate at 10 µg

Lane 4:

Human fetal spleen whole tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 32 kDa

Observed band size: 32 kDa

false

Exposure time: 15s

Supplier Data

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (AB203113)

Blocking/dilution buffer

5% NFDM/TBST.

All lanes:

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113) at 1/2000 dilution

All lanes:

MCF-7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 32 kDa

Observed band size: 32 kDa

false

Exposure time: 3min

Lab

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (AB203113)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113) at 1/2000 dilution

All lanes:

Mouse heart tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 32 kDa

Observed band size: 32 kDa

false

Exposure time: 10s

Lab

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (AB203113)

Blocking buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113) at 1/1000 dilution

All lanes:

Mouse muscle lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 32 kDa

Observed band size: 32 kDa

false

Exposure time: 30s

Carrier free

Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-gamma Sarcoglycan antibody [EPR17862-7]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17862-7

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-gamma Sarcoglycan antibody [EPR17862-7] (ab203113) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P) in Human, Mouse samples.

What is the molecular weight of gamma Sarcoglycan?
Anti-gamma Sarcoglycan [EPR17862-7] (ab203113) specifically detects a band for gamma Sarcoglycan (UniProt: Q13326) at a molecular weight of 32kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR17862-7] also available for your convenience: ab203113, Carrier free - ab251394, Alexa Fluor® 647 - ab315257

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Gamma sarcoglycan also known as γ-sarcoglycan is a transmembrane protein with a molecular mass of approximately 35-50 kDa. It plays a mechanical role by stabilizing muscle cell membranes. Filamentous and inelastic it integrates into the dystrophin-associated glycoprotein complex (DGC) within skeletal and cardiac muscles. Cells express gamma sarcoglycan primarily in muscle tissue making it vital for muscle integrity and function.

Biological function summary

Gamma sarcoglycan acts as a component of the sarcoglycan complex part of the larger dystrophin-glycoprotein complex. This complex connects the cytoskeleton of a muscle fiber to the extracellular matrix maintaining structural stability under mechanical stress. Its role is to ensure force transmission while preventing membrane damage along with proteins like dystrophin beta sarcoglycan and delta sarcoglycan. Gamma sarcoglycan's expression is important for proper muscle contraction and resilience.

Pathways

Gamma sarcoglycan associates with the dystrophin-glycoprotein complex pathway contributing to cellular stability and muscle fiber integrity. It also intersects with the actin cytoskeleton signaling pathway where it modulates structural organization. In these pathways it works alongside dystrophin to secure muscle fibers during contraction and relaxation cycles preventing damage from mechanical strain.

Mutations in the gamma sarcoglycan gene lead to limb-girdle muscular dystrophy type 2C (LGMD2C) characterized by progressive muscle weakness. This genetic disorder causes instability in the sarcoglycan complex resulting in muscle fiber degradation. In another condition dilated cardiomyopathy defects in gamma sarcoglycan disrupt heart muscle function. These diseases often involve interactions with proteins like dystrophin and other sarcoglycans which also contribute to muscle deterioration.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.

See full target information SGCG

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Methods & clinical development 28:284-299 PubMed36816759

2023

Systemic γ-sarcoglycan AAV gene transfer results in dose-dependent correction of muscle deficits in the LGMD 2C/R5 mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Young-Eun Seo,Stephen H Baine,Amber N Kempton,Oliver C Rogers,Sarah Lewis,Kaitlin Adegboye,Alex Haile,Danielle A Griffin,Ellyn L Peterson,Eric R Pozsgai,Rachael A Potter,Louise R Rodino-Klapac

Molecular therapy. Methods & clinical development 13:494-502 PubMed31194043

2019

An AAV-SGCG Dose-Response Study in a γ-Sarcoglycanopathy Mouse Model in the Context of Mechanical Stress.

Applications

Unspecified application

Species

Unspecified reactive species

David Israeli,Jérémie Cosette,Guillaume Corre,Fatima Amor,Jérôme Poupiot,Daniel Stockholm,Marie Montus,Bernard Gjata,Isabelle Richard

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-gamma Sarcoglycan antibody [EPR17862-7]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody [EPR17862-7] (AB203113)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody [EPR17862-7] (AB203113)

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (AB203113)

Immunoprecipitation - Anti-gamma Sarcoglycan antibody [EPR17862-7] (AB203113)

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (AB203113)

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (AB203113)

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (AB203113)

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (AB203113)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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