JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB251394

Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal gamma Sarcoglycan antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 1 publication.

View Alternative Names

Gamma-sarcoglycan, Gamma-SG, 35 kDa dystrophin-associated glycoprotein, 35DAG, SGCG

8 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (AB251394)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (AB251394)

This data was developed using ab203113, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling gamma Sarcoglycan with ab203113 at 1/500 dilution followed by ab97051 Goat Anti-Rabbit IgG H&L (HRP) at a 1/500 dilution. Cell membrane and weak cytoplasmic staining observed. Counter stained with Hematoxylin.

Negative control : Used PBS instead of primary antibody, with secondary ab97051 Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (AB251394)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (AB251394)

This data was developed using ab203113, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling gamma Sarcoglycan with ab203113 at 1/500 dilution followed by ab97051 Goat Anti-Rabbit IgG H&L (HRP) at a 1/500 dilution. No staining is observed on Human colon tissue. Counter stained with Hematoxylin.

Negative control : Used PBS instead of primary antibody, with secondary ab97051 Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (AB251394)
  • IP

Unknown

Immunoprecipitation - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (AB251394)

This data was developed using ab203113, the same antibody clone in a different buffer formulation.

Human gamma Sarcoglycan was immunoprecipitated from 1mg of Human fetal kidney whole cell lysate with ab203113 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab203113 at 1/10000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1 : Human fetal kidney whole cell lysate 10 μg (Input). Lane 2 : ab203113 IP in Human fetal kidney whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab203113 in Human fetal kidney whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 3 Seconds

Blocking/Dilution buffer 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-gamma Sarcoglycan antibody [EPR17862-7] (<a href='/en-us/products/primary-antibodies/gamma-sarcoglycan-antibody-epr17862-7-ab203113'>ab203113</a>)

Predicted band size: 32 kDa

false

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (AB251394)
  • WB

Supplier Data

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (AB251394)

This data was developed using ab203113, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

This protein is expressed in skeletal and heart muscle.

All lanes:

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (<a href='/en-us/products/primary-antibodies/gamma-sarcoglycan-antibody-epr17862-7-ab203113'>ab203113</a>) at 1/10000 dilution

All lanes:

Human skeletal muscle whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 32 kDa

Observed band size: 32 kDa

false

Exposure time: 15s

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (AB251394)
  • WB

Supplier Data

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (AB251394)

This data was developed using ab203113, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

This protein is expressed in skeletal and heart muscle.

ab181602 Anti-GAPDH antibody [EPR16891] control used.

All lanes:

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (<a href='/en-us/products/primary-antibodies/gamma-sarcoglycan-antibody-epr17862-7-ab203113'>ab203113</a>) at 1/2000 dilution

Lane 1:

Human fetal brain whole tissue lysate at 10 µg

Lane 2:

Human fetal heart whole tissue lysate at 10 µg

Lane 3:

Human fetal kidney whole tissue lysate at 10 µg

Lane 4:

Human fetal spleen whole tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 32 kDa

Observed band size: 32 kDa

false

Exposure time: 15s

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (AB251394)
  • WB

Supplier Data

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (AB251394)

This data was developed using ab203113, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (<a href='/en-us/products/primary-antibodies/gamma-sarcoglycan-antibody-epr17862-7-ab203113'>ab203113</a>) at 1/2000 dilution

All lanes:

MCF-7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 32 kDa

Observed band size: 32 kDa

false

Exposure time: 3min

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (AB251394)
  • WB

Lab

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (AB251394)

This data was developed using ab203113, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (<a href='/en-us/products/primary-antibodies/gamma-sarcoglycan-antibody-epr17862-7-ab203113'>ab203113</a>) at 1/2000 dilution

All lanes:

Mouse heart tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 32 kDa

Observed band size: 32 kDa

false

Exposure time: 10s

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (AB251394)
  • WB

Lab

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] - BSA and Azide free (AB251394)

This data was developed using ab203113, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-gamma Sarcoglycan antibody [EPR17862-7] (<a href='/en-us/products/primary-antibodies/gamma-sarcoglycan-antibody-epr17862-7-ab203113'>ab203113</a>) at 1/1000 dilution

All lanes:

Mouse muscle lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 32 kDa

Observed band size: 32 kDa

false

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17862-7

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

WB, IP, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Primary Antibodies

AB189254

Anti-alpha Sarcoglycan antibody [EPR14773]

RabMAb|Recombinant|20ul selling size|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Sarcoglycan antibody [EPR14773] (AB189254)

  • 0
  • 0
Primary Antibodies

AB15277

Anti-Dystrophin antibody

20ul selling size

Immunohistochemistry - Anti-Dystrophin antibody (AB15277)

  • 4
  • 23
Primary Antibodies

AB234587

Anti-DAG1 antibody [IIH6C4]

KO Validated|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAG1 antibody [IIH6C4] (AB234587)

  • 0
  • 0
Primary Antibodies

AB124684

Anti-Dysferlin antibody [JAI-1-49-3]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dysferlin antibody [JAI-1-49-3] (AB124684)

  • 4
  • 4
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column

Product details

ab251394 is the carrier-free version of ab203113.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Gamma sarcoglycan also known as γ-sarcoglycan is a transmembrane protein with a molecular mass of approximately 35-50 kDa. It plays a mechanical role by stabilizing muscle cell membranes. Filamentous and inelastic it integrates into the dystrophin-associated glycoprotein complex (DGC) within skeletal and cardiac muscles. Cells express gamma sarcoglycan primarily in muscle tissue making it vital for muscle integrity and function.
Biological function summary

Gamma sarcoglycan acts as a component of the sarcoglycan complex part of the larger dystrophin-glycoprotein complex. This complex connects the cytoskeleton of a muscle fiber to the extracellular matrix maintaining structural stability under mechanical stress. Its role is to ensure force transmission while preventing membrane damage along with proteins like dystrophin beta sarcoglycan and delta sarcoglycan. Gamma sarcoglycan's expression is important for proper muscle contraction and resilience.

Pathways

Gamma sarcoglycan associates with the dystrophin-glycoprotein complex pathway contributing to cellular stability and muscle fiber integrity. It also intersects with the actin cytoskeleton signaling pathway where it modulates structural organization. In these pathways it works alongside dystrophin to secure muscle fibers during contraction and relaxation cycles preventing damage from mechanical strain.

Mutations in the gamma sarcoglycan gene lead to limb-girdle muscular dystrophy type 2C (LGMD2C) characterized by progressive muscle weakness. This genetic disorder causes instability in the sarcoglycan complex resulting in muscle fiber degradation. In another condition dilated cardiomyopathy defects in gamma sarcoglycan disrupt heart muscle function. These diseases often involve interactions with proteins like dystrophin and other sarcoglycans which also contribute to muscle deterioration.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.
See full target information SGCG
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 22:800 PubMed39731111

2024

Role of PCBP2 in regulating nanovesicles loaded with curcumin to mitigate neuroferroptosis in neural damage caused by heat stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Guo,Yizhan Wu,Guangjun Wang,Jiangwei Liu
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com