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Knockout Tested Rabbit Recombinant Monoclonal SYUG antibody. Suitable for WB and reacts with Human, Mouse, Rat, Transfected cell lysate - Human, Transfected cell lysate - Mouse, Recombinant fragment - Human samples.

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Images

Western blot - Anti-gamma Synuclein/SNCG antibody [EPR26218-79] (AB315442), expandable thumbnail
  • Western blot - Anti-gamma Synuclein/SNCG antibody [EPR26218-79] (AB315442), expandable thumbnail
  • Western blot - Anti-gamma Synuclein/SNCG antibody [EPR26218-79] (AB315442), expandable thumbnail
  • Western blot - Anti-gamma Synuclein/SNCG antibody [EPR26218-79] (AB315442), expandable thumbnail
  • Western blot - Anti-gamma Synuclein/SNCG antibody [EPR26218-79] (AB315442), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)IHC-PICC/IFIPWB
Human
Not recommended
Not recommended
Not recommended
Not recommended
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Tested
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Tested
Recombinant fragment - Human
Not recommended
Not recommended
Not recommended
Not recommended
Tested
Transfected cell lysate - Human
Not recommended
Not recommended
Not recommended
Not recommended
Tested
Transfected cell lysate - Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Tested

Not recommended
Not recommended

Species
Human, Mouse, Rat, Transfected cell lysate - Human, Transfected cell lysate - Mouse, Recombinant fragment - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Transfected cell lysate - Human, Transfected cell lysate - Mouse, Recombinant fragment - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Transfected cell lysate - Human, Transfected cell lysate - Mouse, Recombinant fragment - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Transfected cell lysate - Human, Transfected cell lysate - Mouse, Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Transfected cell lysate - Human
Dilution info
1/1000
Notes

-

Species
Transfected cell lysate - Mouse
Dilution info
1/1000
Notes

-

Species
Recombinant fragment - Human
Dilution info
1/1000
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Plays a role in neurofilament network integrity. May be involved in modulating axonal architecture during development and in the adult. In vitro, increases the susceptibility of neurofilament-H to calcium-dependent proteases (By similarity). May also function in modulating the keratin network in skin. Activates the MAPK and Elk-1 signal transduction pathway (By similarity).

Alternative names

BCSG1, PERSYN, PRSN, SNCG, Gamma-synuclein, Breast cancer-specific gene 1 protein, Persyn, Synoretin, SR

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal SYUG antibody. Suitable for WB and reacts with Human, Mouse, Rat, Transfected cell lysate - Human, Transfected cell lysate - Mouse, Recombinant fragment - Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR26218-79
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Gamma synuclein sometimes referred to as SNCG or gamma-synuclein is a protein with a molecular weight of approximately 13 kDa. It is a member of the synuclein family which also includes alpha and beta synucleins. This protein is highly expressed in the nervous system particularly in the peripheral nervous system and certain regions of the central nervous system. Outside the nervous system gamma synuclein can be found in the retina reproductive tissues and breast tissue among others.

Biological function summary

Gamma synuclein plays a role in modulating neuronal plasticity and may participate in synaptic regulation. It is not known to be a part of a larger protein complex but it interacts with various other proteins influencing neuron differentiation. Although its precise biological functions are not fully characterized studies suggest that gamma synuclein could impact cellular processes like cytoskeletal dynamics and membrane trafficking.

Pathways

The protein gamma synuclein is involved in cellular stress response and neurodegeneration pathways. It is closely related to proteins like alpha synuclein with which it shares structural and functional features. Pathways involving these proteins can influence several processes such as axonal transport and neurotransmitter release. Dysregulation in these pathways often leads to disruptions in cellular homeostasis.

Associated diseases and disorders

The involvement of gamma synuclein is most notably seen in breast cancer and neurodegenerative diseases such as Alzheimer's disease. Researchers have observed that this protein is overexpressed in various tumors where it might facilitate cancer progression and metastasis. In neurodegeneration interaction with alpha synuclein and tau protein could play a role in the pathogenesis where protein aggregation is a characteristic feature. Understanding its role in these diseases helps in charting possible therapeutic avenues.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-gamma Synuclein/SNCG antibody [EPR26218-79] (ab315442), expandable thumbnail

    Western blot - Anti-gamma Synuclein/SNCG antibody [EPR26218-79] (ab315442)

    This antibody does not cross-react with human beta Synuclein and alpha Synuclein.

    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    In Western blot, Anti-alpha + beta Synuclein antibody [EP1646Y] - (Anti-alpha + beta Synuclein antibody [EP1646Y] ab51252) staining at 1/1000 dilution.

    All lanes: Western blot - Anti-gamma Synuclein/SNCG antibody [EPR26218-79] (ab315442) at 1/1000 dilution

    Lane 1: His-tagged human gamma Synuclein recombinant protein at 10 ng with 5% NFDM/TBST

    Lane 2: His-tagged human beta Synuclein recombinant protein at 10 ng with 5% NFDM/TBST

    Lane 3: His-tagged human alpha Synuclein recombinant protein at 10 ng with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 14 kDa, 20 kDa, 19 kDa

    Exposure time: 59s

  • Western blot - Anti-gamma Synuclein/SNCG antibody [EPR26218-79] (ab315442), expandable thumbnail

    Western blot - Anti-gamma Synuclein/SNCG antibody [EPR26218-79] (ab315442)

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    All lanes: Western blot - Anti-gamma Synuclein/SNCG antibody [EPR26218-79] (ab315442) at 1/1000 dilution

    Lane 1: 293T cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 5 µg with 5% NFDM/TBST

    Lane 2: 293T cells transfected with a human gamma Synuclein expression vector containing a myc-His-tag®, whole cell lysate at 5 µg with 5% NFDM/TBST

    Lane 3: 293T cells transfected with a mouse gamma Synuclein expression vector containing a myc-His-tag®, whole cell lysate at 5 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 17 kDa, 19 kDa, 36 kDa

    Exposure time: 6s

  • Western blot - Anti-gamma Synuclein/SNCG antibody [EPR26218-79] (ab315442), expandable thumbnail

    Western blot - Anti-gamma Synuclein/SNCG antibody [EPR26218-79] (ab315442)

    Negative control: liver (PMID:16596223).

    The identity of the higher MW bands are unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-gamma Synuclein/SNCG antibody [EPR26218-79] (ab315442) at 1/1000 dilution

    Lane 1: Mouse hypothalamus tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 2: Mouse liver tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 3: Rat cerebellum tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 4: Rat liver tissue lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 17 kDa, 36 kDa

    Exposure time: 158s

  • Western blot - Anti-gamma Synuclein/SNCG antibody [EPR26218-79] (ab315442), expandable thumbnail

    Western blot - Anti-gamma Synuclein/SNCG antibody [EPR26218-79] (ab315442)

    Negative control: liver (PMID:16596223).

    The identity of the higher MW bands are unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-gamma Synuclein/SNCG antibody [EPR26218-79] (ab315442) at 1/1000 dilution

    Lane 1: Human liver tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 2: Human hypothalamus tissue lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 15 kDa

    Exposure time: 59s

  • Western blot - Anti-gamma Synuclein/SNCG antibody [EPR26218-79] (ab315442), expandable thumbnail

    Western blot - Anti-gamma Synuclein/SNCG antibody [EPR26218-79] (ab315442)

    In Western blot, ab315442 was shown to bind specifically to gamma Synuclein. Target of interest was observed at 15 kDa in wild-type HeLa cell lysates (lane 1) with no signal observed at this size in gamma Synuclein knockout cell line (lane 2) (lane 2, knockout cell line Human SNCG (gamma Synuclein) knockout HeLa cell line ab265623 / knockout cell lysate Human SNCG (gamma Synuclein) knockout HeLa cell lysate ab257694).

    Negative control: HEK-293 (PMID: 12121974).

    The identity of the higher MW bands are unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-gamma Synuclein/SNCG antibody [EPR26218-79] (ab315442) at 1/1000 dilution

    Lane 1: Wild-type HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 2: gamma Synuclein/SNCG knockout HeLa whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 3: SK-BR-3 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 4: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 15 kDa, 36 kDa

    Exposure time: 180s

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Product protocols

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