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Mouse Monoclonal TBG1 antibody. C-terminal. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, African green monkey samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Tubulin gamma-1 chain aa 400 to C-terminus.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [4D11] - C-terminal (AB173831), expandable thumbnail
  • Western blot - Anti-gamma Tubulin antibody [4D11] - C-terminal (AB173831), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [4D11] - C-terminal (AB173831), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [4D11] - C-terminal (AB173831), expandable thumbnail

Publications

Key facts

Isotype

IgG2b

Host species

Mouse

Storage buffer

Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human Tubulin gamma-1 chain aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P23258

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
African green monkey
Expected
Tested
Expected

Tested
Tested

Species

Human

Dilution info

1/20.00000 - 1/200.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, African green monkey

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Species

African green monkey

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50

Notes

-

Expected
Expected

Species

Mouse, African green monkey

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

5 products for Alternative Version

5 products for Alternative Product

Target data

Function

Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation.

Alternative names

Recommended products

Mouse Monoclonal TBG1 antibody. C-terminal. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, African green monkey samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Tubulin gamma-1 chain aa 400 to C-terminus.

Key facts

Isotype

IgG2b

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Fragment Protein within Human Tubulin gamma-1 chain aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P23258
Clone number

4D11

Purification technique

Affinity purification Protein G

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [4D11] - C-terminal (ab173831), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [4D11] - C-terminal (ab173831)

    Immunofluorescence analysis of formalin-fixed, permeabilized A) HeLa cells and B) U2OS, labeling gamma Tubulin (green) using ab173831 at a 1/50 dilution. Cells were washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. F-Actin (red) was stained with DyLight 547 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.

  • Western blot - Anti-gamma Tubulin antibody [4D11] - C-terminal (ab173831), expandable thumbnail

    Western blot - Anti-gamma Tubulin antibody [4D11] - C-terminal (ab173831)

    All lanes: Western blot - Anti-gamma Tubulin antibody [4D11] - C-terminal (ab173831) at 1/1000 dilution

    Lane 1: HeLa cell lysate at 50 µg

    Lane 2: HepG2 cell lysate at 50 µg

    Lane 3: U2OS cell lysate at 50 µg

    Lane 4: K562 cell lysate at 50 µg

    Lane 5: mouse 3T3L1 cell lysate at 50 µg

    Lane 6: mouse spleen lysate at 50 µg

    Lane 7: monkey COS7 cell lysate at 50 µg

    Secondary

    All lanes: goat anti-mouse-HRP at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 51 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [4D11] - C-terminal (ab173831), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [4D11] - C-terminal (ab173831)

    Immunohistochemical analysis of Human breast carcinoma tissue, labeling gamma Tubulin with ab173831 at a 1/20 dilution (left panel) or without primary antibody (right panel). To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [4D11] - C-terminal (ab173831), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [4D11] - C-terminal (ab173831)

    Immunohistochemical analysis of Human colon tissue, labeling gamma Tubulin with ab173831 at a 1/200 dilution (left panel) or without primary antibody (right panel). To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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