Anti-gamma Tubulin antibody [4D11] - C-terminal

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [4D11] - C-terminal (AB173831)

Immunohistochemical analysis of Human breast carcinoma tissue, labeling gamma Tubulin with ab173831 at a 1/20 dilution (left panel) or without primary antibody (right panel). To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [4D11] - C-terminal (AB173831)

Immunofluorescence analysis of formalin-fixed, permeabilized A) HeLa cells and B) U2OS, labeling gamma Tubulin (green) using ab173831 at a 1/50 dilution. Cells were washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. F-Actin (red) was stained with DyLight 547 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [4D11] - C-terminal (AB173831)

Immunohistochemical analysis of Human colon tissue, labeling gamma Tubulin with ab173831 at a 1/200 dilution (left panel) or without primary antibody (right panel). To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

• WB

Supplier Data

Western blot - Anti-gamma Tubulin antibody [4D11] - C-terminal (AB173831)

All lanes:

Western blot - Anti-gamma Tubulin antibody [4D11] - C-terminal (ab173831) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 50 µg

Lane 2:

HepG2 cell lysate at 50 µg

Lane 3:

U2OS cell lysate at 50 µg

Lane 4:

K562 cell lysate at 50 µg

Lane 5:

mouse 3T3L1 cell lysate at 50 µg

Lane 6:

mouse spleen lysate at 50 µg

Lane 7:

monkey COS7 cell lysate at 50 µg

Secondary

All lanes:

goat anti-mouse-HRP at 1/20000 dilution

Predicted band size: 51 kDa

true