Mouse Monoclonal TBG1 antibody. C-terminal. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, African green monkey samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Tubulin gamma-1 chain aa 400 to C-terminus.
IgG2b
Mouse
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA
Liquid
Monoclonal
IHC-P | WB | ICC/IF | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Expected | Tested | Expected |
African green monkey | Expected | Tested | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/20.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, African green monkey | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Species African green monkey | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/50 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, African green monkey | Dilution info Use at an assay dependent concentration. | Notes - |
Select an associated product type
Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation.
TUBG, TUBG, TUBG1, Tubulin gamma-1 chain, Gamma-1-tubulin, Gamma-tubulin complex component 1, GCP-1
Mouse Monoclonal TBG1 antibody. C-terminal. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, African green monkey samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Tubulin gamma-1 chain aa 400 to C-terminus.
IgG2b
Mouse
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA
Liquid
Monoclonal
4D11
Affinity purification Protein G
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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Full details and terms and conditions can be found here:
Terms & Conditions.
Immunofluorescence analysis of formalin-fixed, permeabilized A) HeLa cells and B) U2OS, labeling gamma Tubulin (green) using ab173831 at a 1/50 dilution. Cells were washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. F-Actin (red) was stained with DyLight 547 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.
All lanes: Western blot - Anti-gamma Tubulin antibody [4D11] - C-terminal (ab173831) at 1/1000 dilution
Lane 1: HeLa cell lysate at 50 µg
Lane 2: HepG2 cell lysate at 50 µg
Lane 3: U2OS cell lysate at 50 µg
Lane 4: K562 cell lysate at 50 µg
Lane 5: mouse 3T3L1 cell lysate at 50 µg
Lane 6: mouse spleen lysate at 50 µg
Lane 7: monkey COS7 cell lysate at 50 µg
All lanes: goat anti-mouse-HRP at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 51 kDa
Immunohistochemical analysis of Human breast carcinoma tissue, labeling gamma Tubulin with ab173831 at a 1/20 dilution (left panel) or without primary antibody (right panel). To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Immunohistochemical analysis of Human colon tissue, labeling gamma Tubulin with ab173831 at a 1/200 dilution (left panel) or without primary antibody (right panel). To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
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