Rabbit Polyclonal Gamma-2-tubulin antibody. Suitable for ICC, WB and reacts with Rat, Human, Chinese hamster, Mouse, Dog samples. Cited in 26 publications. Immunogen corresponding to Synthetic Peptide within Xenopus laevis Tubulin gamma-1 chain aa 400 to C-terminus conjugated to Keyhole Limpet Haemocyanin.
IgG
Rabbit
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS
Liquid
Polyclonal
ICC | WB | |
---|---|---|
Human | Tested | Tested |
Mouse | Expected | Tested |
Rat | Tested | Tested |
Chinese hamster | Expected | Tested |
Dog | Expected | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chinese hamster, Mouse, Dog | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chinese hamster | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Species Dog | Dilution info 1/1000 | Notes - |
Select an associated product type
Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation (By similarity).
TUBG2
Tubulin gamma-2 chain, Gamma-2-tubulin, TUBG2
Rabbit Polyclonal Gamma-2-tubulin antibody. Suitable for ICC, WB and reacts with Rat, Human, Chinese hamster, Mouse, Dog samples. Cited in 26 publications. Immunogen corresponding to Synthetic Peptide within Xenopus laevis Tubulin gamma-1 chain aa 400 to C-terminus conjugated to Keyhole Limpet Haemocyanin.
IgG
Rabbit
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS
Liquid
Polyclonal
IgG fraction
Blue Ice
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Storage in frost-free freezers is not recommended.
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Working dilution samples should be discarded if not used within 12 hours.
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This supplementary information is collated from multiple sources and compiled automatically.
Gamma-tubulin an important component in the microtubule nucleation process is also known as Gtub or γ-tub. It weighs approximately 48 kDa. Gamma-tubulin is a significant protein inside the centrosome where it plays a role in the formation of microtubule organizing centers. Scientists often find and express it in abundance within rapidly dividing cells both in humans and other eukaryotes. Researchers employ gamma-tubulin staining techniques to visualize its location and understand its function within the centrosome.
Gamma-tubulin is part of the gamma-tubulin ring complex also known as gamma-TuRC which anchors the microtubule minus ends influencing microtubule stability and organization. This complex functions as a nucleating template for microtubules which are critical for various cell processes including intracellular transport and chromosome separation during cell division. Gamma-tubulin plays an indispensable role in maintaining the correct function of the centrosome an important cellular organelle in cell cycle progression.
Gamma-tubulin participates in processes involving cell cycle regulation and mitotic spindle formation. These processes depend heavily on microtubule dynamics and organization. Gamma-tubulin interacts closely with proteins like centrins and pericentrin within these pathways. Such interactions ensure accurate chromosomal segregation during mitosis highlighting its importance in cellular reproduction and stability.
Abnormal gamma-tubulin expression and function relate to conditions like cancer and neurological disorders. Changes in gamma-tubulin levels can affect the centrosome's function and microtubule dynamics leading to cell cycle dysregulation and tumorigenesis. Moreover it interacts with mutated tumor suppressor proteins like p53 further contributing to cancer progression. Scientists continue to study gamma-tubulin's role in these diseases providing insights into new therapeutic targets.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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Immunocytochemistry analysis of Rat 1 cells labeling gamma Tubulin with ab11321 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, Cy3™ conjugate. Cells were fixed and permeabilized with methanol followed by acetone.
Immunocytochemistry analysis of HeLa cells labeling gamma Tubulin with ab11321 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, Atto-488 conjugate. Cells were fixed and permeabilized with methanol followed by acetone.
ICC/IF image of ab11321 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11321, 5 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in Hek293, HepG2 and MCF7 cells.
All lanes: Western blot - Anti-gamma Tubulin antibody (ab11321) at 1/1000 dilution
Lane 1: HeLa cell lysate
Lane 2: NIH-3T3 cell lysate
Lane 3: MDCK cell lysate
Lane 4: CHO cell lysate
Lane 5: Rat-2 cell lysate
Lane 6: A431 cell lysate
Lane 7: Jurkat cell lysate
Lane 8: U87 cell lysate
Lane 9: C2C12 cell lysate
All lanes: Goat Anti-Rabbit IgG-peroxidase
Predicted band size: 51 kDa
Blocking buffer 5% milk.
All lanes: Western blot - Anti-gamma Tubulin antibody (ab11321) at 1/1000 dilution
Lane 1: Mouse liver tissue lysate at 20 µg
Lane 2: Mouse hepatocytes whole cell lysate at 20 µg
All lanes: Goat anti-rabbit (HRP) secondary antibody at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 50 kDa
Exposure time: 5s
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