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Rabbit Polyclonal Gamma-2-tubulin antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TUBG2 aa 1-200.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody (AB137822), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody (AB137822), expandable thumbnail
  • Western blot - Anti-gamma Tubulin antibody (AB137822), expandable thumbnail
  • Western blot - Anti-gamma Tubulin antibody (AB137822), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody (AB137822), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human TUBG2 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NRH3

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Tested
Mouse
Tested
Tested
Cow
Predicted
Predicted
Dog
Predicted
Predicted
Xenopus laevis
Predicted
Predicted
Xenopus tropicalis
Predicted
Predicted

Tested
Tested

Species

Mouse

Dilution info

1/500 - 1/3000

Notes

-

Species

Human

Dilution info

1/500 - 1/3000

Notes

-

Predicted
Predicted

Species

Cow, Dog, Xenopus laevis, Xenopus tropicalis

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/100 - 1/1000

Notes

PFA or methanol fixation may provide optimal results depending on sample type.

Species

Human

Dilution info

1/100 - 1/1000

Notes

PFA or methanol fixation may provide optimal results depending on sample type.

Predicted
Predicted

Species

Cow, Dog, Xenopus laevis, Xenopus tropicalis

Dilution info

-

Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation (By similarity).

Alternative names

Recommended products

Rabbit Polyclonal Gamma-2-tubulin antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TUBG2 aa 1-200.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human TUBG2 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NRH3
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Gamma-tubulin an important component in the microtubule nucleation process is also known as Gtub or γ-tub. It weighs approximately 48 kDa. Gamma-tubulin is a significant protein inside the centrosome where it plays a role in the formation of microtubule organizing centers. Scientists often find and express it in abundance within rapidly dividing cells both in humans and other eukaryotes. Researchers employ gamma-tubulin staining techniques to visualize its location and understand its function within the centrosome.

Biological function summary

Gamma-tubulin is part of the gamma-tubulin ring complex also known as gamma-TuRC which anchors the microtubule minus ends influencing microtubule stability and organization. This complex functions as a nucleating template for microtubules which are critical for various cell processes including intracellular transport and chromosome separation during cell division. Gamma-tubulin plays an indispensable role in maintaining the correct function of the centrosome an important cellular organelle in cell cycle progression.

Pathways

Gamma-tubulin participates in processes involving cell cycle regulation and mitotic spindle formation. These processes depend heavily on microtubule dynamics and organization. Gamma-tubulin interacts closely with proteins like centrins and pericentrin within these pathways. Such interactions ensure accurate chromosomal segregation during mitosis highlighting its importance in cellular reproduction and stability.

Associated diseases and disorders

Abnormal gamma-tubulin expression and function relate to conditions like cancer and neurological disorders. Changes in gamma-tubulin levels can affect the centrosome's function and microtubule dynamics leading to cell cycle dysregulation and tumorigenesis. Moreover it interacts with mutated tumor suppressor proteins like p53 further contributing to cancer progression. Scientists continue to study gamma-tubulin's role in these diseases providing insights into new therapeutic targets.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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5 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody (ab137822), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody (ab137822)

    Immunofluorescence of paraformaldehyde-fixed 293T cells labelling gamma Tubulin using ab137822 at 1/500 dilution. Gamma Tubulin (green) and alpha Tubulin filaments labelled with gamma Tubulin (red).

  • Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody (ab137822), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody (ab137822)

    Immunofluorescence of methanol-fixed 293T cells labelling gamma Tubulin using ab137822 at 1/500 dilution. Gamma Tubulin (green) and alpha Tubulin filaments labelled with gamma Tubulin (red).

  • Western blot - Anti-gamma Tubulin antibody (ab137822), expandable thumbnail

    Western blot - Anti-gamma Tubulin antibody (ab137822)

    10% SDS PAGE

    All lanes: Western blot - Anti-gamma Tubulin antibody (ab137822) at 1/1000 dilution

    All lanes: MOLT4 cell lysate at 30 µg

    Predicted band size: 51 kDa

  • Western blot - Anti-gamma Tubulin antibody (ab137822), expandable thumbnail

    Western blot - Anti-gamma Tubulin antibody (ab137822)

    10% SDS PAGE

    All lanes: Western blot - Anti-gamma Tubulin antibody (ab137822) at 1/1000 dilution

    All lanes: NIH-3T3 cell lysate at 30 µg

    Predicted band size: 51 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody (ab137822), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody (ab137822)

    Immunofluorescence analysis of paraformaldehyde-fixed mouse brain, labelling gamma Tubulin using ab137822 at 1/200 dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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