Anti-gamma Tubulin antibody (ab11317) is a rabbit polyclonal antibody that is used to detect gamma Tubulin in Western Blot, ICC/IF. Suitable for Chinese hamster, Human, Mouse, Rat samples.
- Over 100 publications
- Trusted since 2004
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- The FEBS journal 290:5704-57192023YAP co-localizes with the mitotic spindle and midbody to safeguard mitotic division in lung-cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShu-Er Chow,Chia-Chi Hsu,Cheng-Ta Yang,Yaa-Jyuhn J MeirPubMed 37549045
- Scientific data 10:4012023Medium-throughput image-based phenotypic siRNA screen to unveil the molecular basis of B cell polarization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDorian Obino,Mathieu Maurin,Florent Dingli,Damarys Loew,Aurianne Lescure,Emmanuel Terriac,Christel Goudot,Odile Malbec,Danielle Lankar,Maria-Isabel Yuseff,Ana-Maria Lennon-Duménil,Hélène D MoreauPubMed 37353541
- Cancer discovery 13:1230-12492023Dimeric p53 Mutant Elicits Unique Tumor-Suppressive Activities through an Altered Metabolic Program.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJovanka Gencel-Augusto,Xiaoping Su,Yuan Qi,Elizabeth M Whitley,Vinod Pant,Shunbin Xiong,Vrutant Shah,Jerome Lin,Encarnacion Perez,Marta L Fiorotto,Iqbal Mahmud,Abhinav K Jain,Philip L Lorenzi,Nicholas E Navin,Ellen R Richie,Guillermina LozanoPubMed 37067911
- Nature communications 14:17152023Distinct dynein complexes defined by DYNLRB1 and DYNLRB2 regulate mitotic and male meiotic spindle bipolarity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuwen He,John P Gillies,Juliana L Zang,Carmen M Córdoba-Beldad,Io Yamamoto,Yasuhiro Fujiwara,Julie Grantham,Morgan E DeSantis,Hiroki ShibuyaPubMed 36973253
- Cell reports 42:1122602023Matrin3 regulates mitotic spindle dynamics by controlling alternative splicing of CDC14B.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBruna R Muys,Roshan L Shrestha,Dimitrios G Anastasakis,Lorinc Pongor,Xiao Ling Li,Ioannis Grammatikakis,Ahsan Polash,Raj Chari,Myriam Gorospe,Curtis C Harris,Mirit I Aladjem,Munira A Basrai,Markus Hafner,Ashish LalPubMed 36924503
- Methods in molecular biology (Clifton, N.J.) 2557:333-3472022In Vitro Methods to Investigate the Disassembly of the Golgi Ribbon During the G2-M Transition of the Cell Cycle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesInmaculada Ayala,Antonino ColanziPubMed 36512225
- Cells 11:2022Autophagy Induced by Toll-like Receptor Ligands Regulates Antigen Extraction and Presentation by B Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJonathan Lagos,Sara Sagadiev,Jheimmy Diaz,Juan Pablo Bozo,Fanny Guzman,Caroline Stefani,Silvana Zanlungo,Mridu Acharya,Maria Isabel YuseffPubMed 36497137
- Virus research 323:1989952022A new host-targeted antiviral cyclolignan (SAU-22.107) for Dengue Virus infection in cell cultures. Potential action mechanisms based on cell imaging.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYaneth M Brand,Vicky Roa-Linares,Carolina Santiago-Dugarte,Esther Del Olmo,José Luis López-Pérez,Liliana Betancur-Galvis,Juan Carlos Gallego-Gómez,Arturo San FelicianoPubMed 36336130
- Methods in molecular biology (Clifton, N.J.) 2566:113-1322022Immunofluorescence Labeling of Skeletal Muscle in Development, Regeneration, and Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarie E Esper,Kasun Kodippili,Michael A RudnickiPubMed 36152246
- Frontiers in genetics 13:10094302022Microtubule modification defects underlie cilium degeneration in cell models of retinitis pigmentosa associated with pre-mRNA splicing factor mutations.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiliya Nazlamova,Suly Saray Villa Vasquez,Jenny Lord,Varshini Karthik,Man-Kim Cheung,Jörn Lakowski,Gabrielle WhewayPubMed 36176300
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.87% Sodium chloride, 0.42% Tripotassium orthophosphate, 0.0268% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human TUBG2 aa 1-100 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NRH3
Consider this alternative
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Rat | Tested | Tested |
| Chinese hamster | Tested | Expected |
| Drosophila melanogaster | Predicted | Predicted |
| Sea urchin | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Chinese hamster | Dilution info 1/1000 | Notes This concentration was determined for A431 cultured cells extract. Block with 5% BSA in TBST (not milk). Detects a band of approximately 48 kDa. Additional bands can occasionally be seen. |
Species Rat | Dilution info 1/1000 | Notes This concentration was determined for A431 cultured cells extract. Block with 5% BSA in TBST (not milk). Detects a band of approximately 48 kDa. Additional bands can occasionally be seen. |
Species Human | Dilution info 1/1000 | Notes This concentration was determined for A431 cultured cells extract. Block with 5% BSA in TBST (not milk). Detects a band of approximately 48 kDa. Additional bands can occasionally be seen. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Drosophila melanogaster, Sea urchin | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/5000 | Notes It is recommended to fix cells with methanol/acetone. |
Species Rat | Dilution info - | Notes It is recommended to fix cells with methanol/acetone. |
Species Human | Dilution info 1/5000 | Notes It is recommended to fix cells with methanol/acetone. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Chinese hamster | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Drosophila melanogaster, Sea urchin | Dilution info - | Notes - |
Associated Products
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Target data
Function
Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation (By similarity).
Alternative names
Tubulin gamma-2 chain, Gamma-2-tubulin, TUBG2
Recommended products
Anti-gamma Tubulin antibody (ab11317) is a rabbit polyclonal antibody that is used to detect gamma Tubulin in Western Blot, ICC/IF. Suitable for Chinese hamster, Human, Mouse, Rat samples.
- Over 100 publications
- Trusted since 2004
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.87% Sodium chloride, 0.42% Tripotassium orthophosphate, 0.0268% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human TUBG2 aa 1-100 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NRH3
- Purity
- IgG fraction
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-gamma Tubulin antibody (ab11317) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF) in Chinese hamster, Human, Mouse, Rat samples.
Trusted by the scientific community
Anti-gamma Tubulin (ab11317) was first used in a scientific publication in 2004 and has been cited over 100 times in peer-reviewed journals.
Reviewed by scientists
Anti-gamma Tubulin (ab11317) has over 15 independent reviews from customers.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Gamma-tubulin an important component in the microtubule nucleation process is also known as Gtub or γ-tub. It weighs approximately 48 kDa. Gamma-tubulin is a significant protein inside the centrosome where it plays a role in the formation of microtubule organizing centers. Scientists often find and express it in abundance within rapidly dividing cells both in humans and other eukaryotes. Researchers employ gamma-tubulin staining techniques to visualize its location and understand its function within the centrosome.
- Biological function summary
Gamma-tubulin is part of the gamma-tubulin ring complex also known as gamma-TuRC which anchors the microtubule minus ends influencing microtubule stability and organization. This complex functions as a nucleating template for microtubules which are critical for various cell processes including intracellular transport and chromosome separation during cell division. Gamma-tubulin plays an indispensable role in maintaining the correct function of the centrosome an important cellular organelle in cell cycle progression.
- Pathways
Gamma-tubulin participates in processes involving cell cycle regulation and mitotic spindle formation. These processes depend heavily on microtubule dynamics and organization. Gamma-tubulin interacts closely with proteins like centrins and pericentrin within these pathways. Such interactions ensure accurate chromosomal segregation during mitosis highlighting its importance in cellular reproduction and stability.
- Associated diseases and disorders
Abnormal gamma-tubulin expression and function relate to conditions like cancer and neurological disorders. Changes in gamma-tubulin levels can affect the centrosome's function and microtubule dynamics leading to cell cycle dysregulation and tumorigenesis. Moreover it interacts with mutated tumor suppressor proteins like p53 further contributing to cancer progression. Scientists continue to study gamma-tubulin's role in these diseases providing insights into new therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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5 product images
Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody - Centrosome Marker (ab11317)
Gamma Tubulin Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-gamma Tubulin antibody
Immunocytochemistry/Immunofluorescence analysis of NIH3T3 cells labelling gamma Tubulin (green) with ab11317 at a dilution of 1/1500. A Atto448 dye conjugated goat anti-rabbit IgG was used as the seondary antibody. Actin was stained with phalloidin rhodamine conjugate (red). Nuclear staining with DAPI (blue).
Western blot - Anti-gamma Tubulin antibody - Centrosome Marker (ab11317)
Gamma Tubulin Western blot staining using rabbit Anti-gamma Tubulin antibody
All lanes: Western blot - Anti-gamma Tubulin antibody - Centrosome Marker (ab11317) at 1/1000 dilution
Lane 1: A431 cell lysate
Lane 2: HeLa cell lysate
Lane 3: Jurkat cell lysate
Lane 4: C2C12 cell lysate
Lane 5: Rat2 cell lysate
Lane 6: U87 cell lysate
Lane 7: NIH3T3 cell lysate
Lane 8: CHO cell lysate
SecondaryAll lanes: Peroxidase-conjugated goat anti-rabbit IgG
Predicted band size: 51 kDa
Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody - Centrosome Marker (ab11317)
Gamma Tubulin Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-gamma Tubulin antibody
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling gamma Tubulin (green) with ab11317 at a dilution of 1/1500. A Atto448 dye conjugated goat anti-rabbit IgG was used as the seondary antibody. Actin was stained with phalloidin rhodamine conjugate (red). Nuclear staining with DAPI (blue).
Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody - Centrosome Marker (ab11317)
Gamma Tubulin Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-gamma Tubulin antibody
Immunocytochemistry/Immunofluorescence analysis of Rat2 cells labelling gamma Tubulin (green) with ab11317 at a dilution of 1/1500. A Atto448 dye conjugated goat anti-rabbit IgG was used as the seondary antibody. Actin was stained with phalloidin rhodamine conjugate (red). Nuclear staining with DAPI (blue).
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-gamma Tubulin antibody - Centrosome Marker (ab11317)
Gamma Tubulin Western blot staining using rabbit Anti-gamma Tubulin antibody
gamma Tubulin western blot using anti-gamma Tubulin antibody ab11317. Publication image and figure legend from Moser, S. C., Bensaddek, D., et al., 2013, Dev Cell, PubMed 23932902.
ab11317 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab11317 please see the product overview.
Changes in PHD1 Activity Alter Cep192 Protein Levels(A) Hypoxia leads to the increase of Cep192 levels. U2OS cells were exposed to 1% O2 for the indicated time points. Whole-cell lysates were blotted for the indicated proteins.(B) Low-oxygen tension increases Cep192-GFP levels but not Cep192-LAA-GFP levels. HeLa Kyoto cells stably expressing Cep192-GFP and Cep192-LAA-GFP were exposed to 1% O2 for the indicated time points. Whole-cell lysates were blotted for the indicated proteins.(C) Inactivation of PHD1 increases Cep192 levels. Cells were treated with PHD1 siRNA and lysates were blotted for the indicated proteins.(D) Cells were treated with DFX or MG132 and lysates were blotted for the indicated proteins.(E) Inactivation of PHD1 increases Cep192-GFP levels but not that of the Cep192-LAA-GFP mutants. Cells were treated with PHD1 siRNA and lysates were blotted for the indicated proteins.See also Figures S5 and S6.
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