Anti-gamma Tubulin antibody - Centrosome Marker

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody - Centrosome Marker (AB11317)

Immunocytochemistry/Immunofluorescence analysis of NIH3T3 cells labelling gamma Tubulin (green) with ab11317 at a dilution of 1/1500. A Atto448 dye conjugated goat anti-rabbit IgG was used as the seondary antibody. Actin was stained with phalloidin rhodamine conjugate (red). Nuclear staining with DAPI (blue).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody - Centrosome Marker (AB11317)

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling gamma Tubulin (green) with ab11317 at a dilution of 1/1500. A Atto448 dye conjugated goat anti-rabbit IgG was used as the seondary antibody. Actin was stained with phalloidin rhodamine conjugate (red). Nuclear staining with DAPI (blue).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody - Centrosome Marker (AB11317)

Immunocytochemistry/Immunofluorescence analysis of Rat2 cells labelling gamma Tubulin (green) with ab11317 at a dilution of 1/1500. A Atto448 dye conjugated goat anti-rabbit IgG was used as the seondary antibody. Actin was stained with phalloidin rhodamine conjugate (red). Nuclear staining with DAPI (blue).

• WB

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Western blot - Anti-gamma Tubulin antibody - Centrosome Marker (AB11317)

All lanes:

Western blot - Anti-gamma Tubulin antibody - Centrosome Marker (ab11317) at 1/1000 dilution

Lane 1:

A431 cell lysate

Lane 2:

HeLa cell lysate

Lane 3:

Jurkat cell lysate

Lane 4:

C2C12 cell lysate

Lane 5:

Rat2 cell lysate

Lane 6:

U87 cell lysate

Lane 7:

NIH3T3 cell lysate

Lane 8:

CHO cell lysate

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG

Predicted band size: 51 kDa

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• WB

CiteAb

Western blot - Anti-gamma Tubulin antibody - Centrosome Marker (AB11317)

gamma Tubulin western blot using anti-gamma Tubulin antibody ab11317. Publication image and figure legend from Moser, S. C., Bensaddek, D., et al., 2013, Dev Cell, PubMed 23932902.

ab11317 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab11317 please see the product overview.

Changes in PHD1 Activity Alter Cep192 Protein Levels(A) Hypoxia leads to the increase of Cep192 levels. U2OS cells were exposed to 1% O2 for the indicated time points. Whole-cell lysates were blotted for the indicated proteins.(B) Low-oxygen tension increases Cep192-GFP levels but not Cep192-LAA-GFP levels. HeLa Kyoto cells stably expressing Cep192-GFP and Cep192-LAA-GFP were exposed to 1% O2 for the indicated time points. Whole-cell lysates were blotted for the indicated proteins.(C) Inactivation of PHD1 increases Cep192 levels. Cells were treated with PHD1 siRNA and lysates were blotted for the indicated proteins.(D) Cells were treated with DFX or MG132 and lysates were blotted for the indicated proteins.(E) Inactivation of PHD1 increases Cep192-GFP levels but not that of the Cep192-LAA-GFP mutants. Cells were treated with PHD1 siRNA and lysates were blotted for the indicated proteins.See also Figures S5 and S6.

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