Rabbit Polyclonal Gamma-2-tubulin antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TUBG2 aa 1-200.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human TUBG2 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NRH3
Consider this alternative
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Tested |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted |
| Xenopus tropicalis | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Dog, Xenopus laevis, Xenopus tropicalis | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/1000 | Notes PFA or methanol fixation may provide optimal results depending on sample type. |
Species Human | Dilution info 1/100 - 1/1000 | Notes PFA or methanol fixation may provide optimal results depending on sample type. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Dog, Xenopus laevis, Xenopus tropicalis | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation (By similarity).
Alternative names
Tubulin gamma-2 chain, Gamma-2-tubulin, TUBG2
Recommended products
Rabbit Polyclonal Gamma-2-tubulin antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TUBG2 aa 1-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human TUBG2 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NRH3
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Gamma-tubulin an important component in the microtubule nucleation process is also known as Gtub or γ-tub. It weighs approximately 48 kDa. Gamma-tubulin is a significant protein inside the centrosome where it plays a role in the formation of microtubule organizing centers. Scientists often find and express it in abundance within rapidly dividing cells both in humans and other eukaryotes. Researchers employ gamma-tubulin staining techniques to visualize its location and understand its function within the centrosome.
- Biological function summary
Gamma-tubulin is part of the gamma-tubulin ring complex also known as gamma-TuRC which anchors the microtubule minus ends influencing microtubule stability and organization. This complex functions as a nucleating template for microtubules which are critical for various cell processes including intracellular transport and chromosome separation during cell division. Gamma-tubulin plays an indispensable role in maintaining the correct function of the centrosome an important cellular organelle in cell cycle progression.
- Pathways
Gamma-tubulin participates in processes involving cell cycle regulation and mitotic spindle formation. These processes depend heavily on microtubule dynamics and organization. Gamma-tubulin interacts closely with proteins like centrins and pericentrin within these pathways. Such interactions ensure accurate chromosomal segregation during mitosis highlighting its importance in cellular reproduction and stability.
- Associated diseases and disorders
Abnormal gamma-tubulin expression and function relate to conditions like cancer and neurological disorders. Changes in gamma-tubulin levels can affect the centrosome's function and microtubule dynamics leading to cell cycle dysregulation and tumorigenesis. Moreover it interacts with mutated tumor suppressor proteins like p53 further contributing to cancer progression. Scientists continue to study gamma-tubulin's role in these diseases providing insights into new therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody - Centrosome Marker (ab137822)
Gamma Tubulin Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-gamma Tubulin antibody
Immunofluorescence of paraformaldehyde-fixed 293T cells labelling gamma Tubulin using ab137822 at 1/500 dilution. Gamma Tubulin (green) and alpha Tubulin filaments labelled with gamma Tubulin (red).
Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody - Centrosome Marker (ab137822)
Gamma Tubulin Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-gamma Tubulin antibody
Immunofluorescence of methanol-fixed 293T cells labelling gamma Tubulin using ab137822 at 1/500 dilution. Gamma Tubulin (green) and alpha Tubulin filaments labelled with gamma Tubulin (red).
Western blot - Anti-gamma Tubulin antibody - Centrosome Marker (ab137822)
Gamma Tubulin Western blot staining of MOLT4 cell lysate using rabbit Anti-gamma Tubulin antibody
10% SDS PAGE
All lanes: Western blot - Anti-gamma Tubulin antibody - Centrosome Marker (ab137822) at 1/1000 dilution
All lanes: MOLT4 cell lysate at 30 µg
Predicted band size: 51 kDa
Western blot - Anti-gamma Tubulin antibody - Centrosome Marker (ab137822)
Gamma Tubulin Western blot staining of NIH-3T3 cell lysate using rabbit Anti-gamma Tubulin antibody
10% SDS PAGE
All lanes: Western blot - Anti-gamma Tubulin antibody - Centrosome Marker (ab137822) at 1/1000 dilution
All lanes: NIH-3T3 cell lysate at 30 µg
Predicted band size: 51 kDa
Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody - Centrosome Marker (ab137822)
Gamma Tubulin Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-gamma Tubulin antibody
Immunofluorescence analysis of paraformaldehyde-fixed mouse brain, labelling gamma Tubulin using ab137822 at 1/200 dilution.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com