Rabbit Recombinant Monoclonal Gamma-2-tubulin antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples.
Images
![Western blot - Anti-gamma Tubulin antibody [EPR13872] - BSA and Azide free (AB250240), expandable thumbnail](https://content.abcam.com/products/images/gamma-tubulin-antibody-epr13872-bsa-and-azide-free-ab250240--western-blot-img115113.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-gamma Tubulin antibody [EPR13872] - BSA and Azide free (AB250240)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [EPR13872] - BSA and Azide free (AB250240), expandable thumbnail](https://content.abcam.com/products/images/gamma-tubulin-antibody-epr13872-bsa-and-azide-free-ab250240--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img123160.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [EPR13872] - BSA and Azide free (AB250240)")
![Flow Cytometry (Intracellular) - Anti-gamma Tubulin antibody [EPR13872] - BSA and Azide free (AB250240), expandable thumbnail](https://content.abcam.com/products/images/gamma-tubulin-antibody-epr13872-bsa-and-azide-free-ab250240--flow-cytometry-intracellular-img90682.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-gamma Tubulin antibody [EPR13872] - BSA and Azide free (AB250240)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | Flow Cyt (Intra) | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes For antigen retrieval, heat up to 98 degree C, below boiling, and then let cool for 10-20 minutes. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Dog | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Dog | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Dog | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation (By similarity).
Alternative names
Tubulin gamma-2 chain, Gamma-2-tubulin, TUBG2
Recommended products
Rabbit Recombinant Monoclonal Gamma-2-tubulin antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR13872
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab250240 is the carrier-free version of Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker ab180595.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Gamma-tubulin an important component in the microtubule nucleation process is also known as Gtub or γ-tub. It weighs approximately 48 kDa. Gamma-tubulin is a significant protein inside the centrosome where it plays a role in the formation of microtubule organizing centers. Scientists often find and express it in abundance within rapidly dividing cells both in humans and other eukaryotes. Researchers employ gamma-tubulin staining techniques to visualize its location and understand its function within the centrosome.
- Biological function summary
Gamma-tubulin is part of the gamma-tubulin ring complex also known as gamma-TuRC which anchors the microtubule minus ends influencing microtubule stability and organization. This complex functions as a nucleating template for microtubules which are critical for various cell processes including intracellular transport and chromosome separation during cell division. Gamma-tubulin plays an indispensable role in maintaining the correct function of the centrosome an important cellular organelle in cell cycle progression.
- Pathways
Gamma-tubulin participates in processes involving cell cycle regulation and mitotic spindle formation. These processes depend heavily on microtubule dynamics and organization. Gamma-tubulin interacts closely with proteins like centrins and pericentrin within these pathways. Such interactions ensure accurate chromosomal segregation during mitosis highlighting its importance in cellular reproduction and stability.
- Associated diseases and disorders
Abnormal gamma-tubulin expression and function relate to conditions like cancer and neurological disorders. Changes in gamma-tubulin levels can affect the centrosome's function and microtubule dynamics leading to cell cycle dysregulation and tumorigenesis. Moreover it interacts with mutated tumor suppressor proteins like p53 further contributing to cancer progression. Scientists continue to study gamma-tubulin's role in these diseases providing insights into new therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-gamma Tubulin antibody [EPR13872] - BSA and Azide free (ab250240), expandable thumbnail](https://content.abcam.com/products/images/gamma-tubulin-antibody-epr13872-bsa-and-azide-free-ab250240--western-blot-img115113.jpg "Western blot - Anti-gamma Tubulin antibody [EPR13872] - BSA and Azide free (ab250240)")
Western blot - Anti-gamma Tubulin antibody [EPR13872] - BSA and Azide free (ab250240)
This data was developed using Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker ab180595, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker ab180595) at 1/2000 dilution
Lane 1: HeLa cell lysate at 20 µg
Lane 2: Jurkat cell lysate at 20 µg
Lane 3: A431 cell lysate at 20 µg
Lane 4: 293 cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated at 1/1000 dilution
Predicted band size: 51 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [EPR13872] - BSA and Azide free (ab250240), expandable thumbnail](https://content.abcam.com/products/images/gamma-tubulin-antibody-epr13872-bsa-and-azide-free-ab250240--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img123160.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [EPR13872] - BSA and Azide free (ab250240)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [EPR13872] - BSA and Azide free (ab250240)
This data was developed using Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker ab180595, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human colon tissue labeling gamma Tubulin with Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker ab180595 at a 1/100 dilution. Prediluted ImmunoHistoprobe HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-gamma Tubulin antibody [EPR13872] - BSA and Azide free (ab250240), expandable thumbnail](https://content.abcam.com/products/images/gamma-tubulin-antibody-epr13872-bsa-and-azide-free-ab250240--flow-cytometry-intracellular-img90682.jpg "Flow Cytometry (Intracellular) - Anti-gamma Tubulin antibody [EPR13872] - BSA and Azide free (ab250240)")
Flow Cytometry (Intracellular) - Anti-gamma Tubulin antibody [EPR13872] - BSA and Azide free (ab250240)
This data was developed using Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker ab180595, the same antibody clone in a different buffer formulation.
Intracellular Flow Cytometry analysis of Jurkat cells using Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker ab180595 at a 1/140 dilution (red) or a rabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary used at a 1/150 dilution.
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