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Rabbit Recombinant Monoclonal TBG1 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IP, IHC-P and reacts with Chicken, Human, Dog, Cow, Mouse, Rat, Hamster, African green monkey, Zebrafish, Xenopus tropicalis samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [EPR16793] - BSA and Azide free (AB240216), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [EPR16793] - BSA and Azide free (AB240216), expandable thumbnail
  • Immunoprecipitation - Anti-gamma Tubulin antibody [EPR16793] - BSA and Azide free (AB240216), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [EPR16793] - BSA and Azide free (AB240216), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [EPR16793] - BSA and Azide free (AB240216), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)IPIHC-P
Human
Tested
Tested
Expected
Tested
Expected
Mouse
Tested
Expected
Expected
Expected
Tested
Rat
Tested
Expected
Expected
Expected
Expected
African green monkey
Tested
Expected
Expected
Expected
Expected
Chicken
Tested
Expected
Tested
Expected
Expected
Cow
Tested
Expected
Expected
Expected
Expected
Dog
Tested
Expected
Expected
Expected
Expected
Fish
Predicted
Predicted
Predicted
Predicted
Predicted
Hamster
Tested
Expected
Expected
Expected
Expected
Monkey
Predicted
Predicted
Predicted
Predicted
Predicted
Xenopus tropicalis
Tested
Expected
Expected
Expected
Expected
Zebrafish
Tested
Expected
Expected
Expected
Expected

Tested
Tested

Species

Chicken

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

-

Notes

-

Species

Dog

Dilution info

-

Notes

-

Species

Cow

Dilution info

-

Notes

-

Species

Mouse

Dilution info

-

Notes

-

Species

Rat

Dilution info

-

Notes

-

Species

Hamster

Dilution info

-

Notes

-

Species

African green monkey

Dilution info

-

Notes

-

Species

Zebrafish

Dilution info

-

Notes

-

Species

Xenopus tropicalis

Dilution info

-

Notes

-

Predicted
Predicted

Species

Fish, Monkey

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Chicken, Dog, Cow, Mouse, Rat, Hamster, African green monkey, Zebrafish, Xenopus tropicalis

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Fish, Monkey

Dilution info

-

Notes

-

Tested
Tested

Species

Chicken

Dilution info

-

Notes

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Human, Dog, Cow, Mouse, Rat, Hamster, African green monkey, Zebrafish, Xenopus tropicalis

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Fish, Monkey

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Chicken, Dog, Cow, Mouse, Rat, Hamster, African green monkey, Zebrafish, Xenopus tropicalis

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Fish, Monkey

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Chicken, Human, Dog, Cow, Rat, Hamster, African green monkey, Zebrafish, Xenopus tropicalis

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Fish, Monkey

Dilution info

-

Notes

-

Associated Products

Select an associated product type

10 products for Alternative Product

2 products for Alternative Version

Target data

Function

Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal TBG1 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IP, IHC-P and reacts with Chicken, Human, Dog, Cow, Mouse, Rat, Hamster, African green monkey, Zebrafish, Xenopus tropicalis samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR16793

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab240216 is the carrier-free version of Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker ab179503.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Gamma-tubulin an important component in the microtubule nucleation process is also known as Gtub or γ-tub. It weighs approximately 48 kDa. Gamma-tubulin is a significant protein inside the centrosome where it plays a role in the formation of microtubule organizing centers. Scientists often find and express it in abundance within rapidly dividing cells both in humans and other eukaryotes. Researchers employ gamma-tubulin staining techniques to visualize its location and understand its function within the centrosome.

Biological function summary

Gamma-tubulin is part of the gamma-tubulin ring complex also known as gamma-TuRC which anchors the microtubule minus ends influencing microtubule stability and organization. This complex functions as a nucleating template for microtubules which are critical for various cell processes including intracellular transport and chromosome separation during cell division. Gamma-tubulin plays an indispensable role in maintaining the correct function of the centrosome an important cellular organelle in cell cycle progression.

Pathways

Gamma-tubulin participates in processes involving cell cycle regulation and mitotic spindle formation. These processes depend heavily on microtubule dynamics and organization. Gamma-tubulin interacts closely with proteins like centrins and pericentrin within these pathways. Such interactions ensure accurate chromosomal segregation during mitosis highlighting its importance in cellular reproduction and stability.

Associated diseases and disorders

Abnormal gamma-tubulin expression and function relate to conditions like cancer and neurological disorders. Changes in gamma-tubulin levels can affect the centrosome's function and microtubule dynamics leading to cell cycle dysregulation and tumorigenesis. Moreover it interacts with mutated tumor suppressor proteins like p53 further contributing to cancer progression. Scientists continue to study gamma-tubulin's role in these diseases providing insights into new therapeutic targets.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com