Rabbit Recombinant Monoclonal TBG1 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IP, IHC-P and reacts with Chicken, Human, Dog, Cow, Mouse, Rat, Hamster, African green monkey, Zebrafish, Xenopus tropicalis samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
WB | ICC/IF | Flow Cyt (Intra) | IP | IHC-P | |
---|---|---|---|---|---|
Human | Tested | Tested | Expected | Tested | Expected |
Mouse | Tested | Expected | Expected | Expected | Tested |
Rat | Tested | Expected | Expected | Expected | Expected |
African green monkey | Tested | Expected | Expected | Expected | Expected |
Chicken | Tested | Expected | Tested | Expected | Expected |
Cow | Tested | Expected | Expected | Expected | Expected |
Dog | Tested | Expected | Expected | Expected | Expected |
Fish | Predicted | Predicted | Predicted | Predicted | Predicted |
Hamster | Tested | Expected | Expected | Expected | Expected |
Monkey | Predicted | Predicted | Predicted | Predicted | Predicted |
Xenopus tropicalis | Tested | Expected | Expected | Expected | Expected |
Zebrafish | Tested | Expected | Expected | Expected | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Chicken | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes - |
Species Dog | Dilution info - | Notes - |
Species Cow | Dilution info - | Notes - |
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
Species Hamster | Dilution info - | Notes - |
Species African green monkey | Dilution info - | Notes - |
Species Zebrafish | Dilution info - | Notes - |
Species Xenopus tropicalis | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Fish, Monkey | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chicken, Dog, Cow, Mouse, Rat, Hamster, African green monkey, Zebrafish, Xenopus tropicalis | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Fish, Monkey | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chicken | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species | Dilution info | Notes |
---|---|---|
Species Human, Dog, Cow, Mouse, Rat, Hamster, African green monkey, Zebrafish, Xenopus tropicalis | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Fish, Monkey | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chicken, Dog, Cow, Mouse, Rat, Hamster, African green monkey, Zebrafish, Xenopus tropicalis | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Fish, Monkey | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Chicken, Human, Dog, Cow, Rat, Hamster, African green monkey, Zebrafish, Xenopus tropicalis | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Fish, Monkey | Dilution info - | Notes - |
Select an associated product type
Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation.
TUBG, TUBG, TUBG1, Tubulin gamma-1 chain, Gamma-1-tubulin, Gamma-tubulin complex component 1, GCP-1
Rabbit Recombinant Monoclonal TBG1 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IP, IHC-P and reacts with Chicken, Human, Dog, Cow, Mouse, Rat, Hamster, African green monkey, Zebrafish, Xenopus tropicalis samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
Yes
EPR16793
Affinity purification Protein A
Blue Ice
+4°C
+4°C
Do Not Freeze
ab240216 is the carrier-free version of Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker ab179503.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This supplementary information is collated from multiple sources and compiled automatically.
Gamma-tubulin an important component in the microtubule nucleation process is also known as Gtub or γ-tub. It weighs approximately 48 kDa. Gamma-tubulin is a significant protein inside the centrosome where it plays a role in the formation of microtubule organizing centers. Scientists often find and express it in abundance within rapidly dividing cells both in humans and other eukaryotes. Researchers employ gamma-tubulin staining techniques to visualize its location and understand its function within the centrosome.
Gamma-tubulin is part of the gamma-tubulin ring complex also known as gamma-TuRC which anchors the microtubule minus ends influencing microtubule stability and organization. This complex functions as a nucleating template for microtubules which are critical for various cell processes including intracellular transport and chromosome separation during cell division. Gamma-tubulin plays an indispensable role in maintaining the correct function of the centrosome an important cellular organelle in cell cycle progression.
Gamma-tubulin participates in processes involving cell cycle regulation and mitotic spindle formation. These processes depend heavily on microtubule dynamics and organization. Gamma-tubulin interacts closely with proteins like centrins and pericentrin within these pathways. Such interactions ensure accurate chromosomal segregation during mitosis highlighting its importance in cellular reproduction and stability.
Abnormal gamma-tubulin expression and function relate to conditions like cancer and neurological disorders. Changes in gamma-tubulin levels can affect the centrosome's function and microtubule dynamics leading to cell cycle dysregulation and tumorigenesis. Moreover it interacts with mutated tumor suppressor proteins like p53 further contributing to cancer progression. Scientists continue to study gamma-tubulin's role in these diseases providing insights into new therapeutic targets.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling gamma Tubulin with Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker ab179503 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/400 dilution (green). Cytoplasmic and centrosome staining on HeLa cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows;
1. Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker ab179503 at 1/500 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
2. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker ab179503).
Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling gamma Tubulin with Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker ab179503 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasmic staining on epithelial cells of mouse testis is observed. Counter stained with Hematoxylin.
Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker ab179503).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Gamma Tubulin was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker ab179503 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker ab179503 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as secondary antibody at 1/1500 dilution. Lane 1: HeLa whole cell extract. Lane 2: PBS instead of HeLa whole cell extract.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker ab179503).
All lanes: Immunoprecipitation - Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker (Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker ab179503)
Predicted band size: 51 kDa
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling gamma Tubulin with Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker ab179503 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasmic staining on tumor cells of breast carcinoma is observed. Counter stained with Hematoxylin.
Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker ab179503).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling gamma Tubulin with Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker ab179503 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasm staining on epithelial cells of rat testis is observed. Counter stained with Hematoxylin.
Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker ab179503).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed UMNSAH/DF-1 (Transformed chicken embyronic fibroblast cells) cells labeling gamma Tubulin with Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker ab179503 at 1/80 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker ab179503).
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