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Mouse Monoclonal Gamma-2-tubulin antibody. Centrosome marker. Suitable for ICC/IF, WB and reacts with Human, Chinese hamster, Mouse, Chicken, Dog, Cow, African green monkey samples. Cited in 178 publications. Immunogen corresponding to Synthetic Peptide within Human TUBG2 aa 1-100 conjugated to Keyhole Limpet Haemocyanin.


Images

Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (AB11316), expandable thumbnail
  • Western blot - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (AB11316), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (AB11316), expandable thumbnail
  • Western blot - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (AB11316), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Synthetic Peptide within Human TUBG2 aa 1-100 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NRH3

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWB
Human
Tested
Tested
Mouse
Expected
Tested
African green monkey
Expected
Tested
Chicken
Expected
Tested
Chinese hamster
Expected
Tested
Cow
Expected
Tested
Dog
Expected
Tested

Tested
Tested

Species

Human

Dilution info

1-2 µg/mL

Notes

-

Expected
Expected

Species

Chinese hamster, Mouse, Chicken, Dog, Cow, African green monkey

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Chinese hamster

Dilution info

1/10000

Notes

-

Species

Mouse

Dilution info

1/10000

Notes

-

Species

African green monkey

Dilution info

1/10000

Notes

-

Species

Chicken

Dilution info

1/10000

Notes

-

Species

Human

Dilution info

1/10000

Notes

-

Species

Dog

Dilution info

1/10000

Notes

-

Species

Cow

Dilution info

1/10000

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation (By similarity).

Alternative names

Recommended products

Mouse Monoclonal Gamma-2-tubulin antibody. Centrosome marker. Suitable for ICC/IF, WB and reacts with Human, Chinese hamster, Mouse, Chicken, Dog, Cow, African green monkey samples. Cited in 178 publications. Immunogen corresponding to Synthetic Peptide within Human TUBG2 aa 1-100 conjugated to Keyhole Limpet Haemocyanin.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Synthetic Peptide within Human TUBG2 aa 1-100 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NRH3
Clone number

GTU-88

Purification technique

Proprietary technique

Concentration
Loading...
Purification notes

Purified from Tissue Culture Supernatant.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product was changed from ascites to tissue culture supernatant on 18th October 2016. The following lots are from ascites and are still in stock as of 18th October 2016 (GR286332 and GR225007). Lot numbers higher than GR286332 will be from tissue culture supernatant. Please note that dilutions may need to be adjusted accordingly.

Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Gamma-tubulin an important component in the microtubule nucleation process is also known as Gtub or γ-tub. It weighs approximately 48 kDa. Gamma-tubulin is a significant protein inside the centrosome where it plays a role in the formation of microtubule organizing centers. Scientists often find and express it in abundance within rapidly dividing cells both in humans and other eukaryotes. Researchers employ gamma-tubulin staining techniques to visualize its location and understand its function within the centrosome.

Biological function summary

Gamma-tubulin is part of the gamma-tubulin ring complex also known as gamma-TuRC which anchors the microtubule minus ends influencing microtubule stability and organization. This complex functions as a nucleating template for microtubules which are critical for various cell processes including intracellular transport and chromosome separation during cell division. Gamma-tubulin plays an indispensable role in maintaining the correct function of the centrosome an important cellular organelle in cell cycle progression.

Pathways

Gamma-tubulin participates in processes involving cell cycle regulation and mitotic spindle formation. These processes depend heavily on microtubule dynamics and organization. Gamma-tubulin interacts closely with proteins like centrins and pericentrin within these pathways. Such interactions ensure accurate chromosomal segregation during mitosis highlighting its importance in cellular reproduction and stability.

Associated diseases and disorders

Abnormal gamma-tubulin expression and function relate to conditions like cancer and neurological disorders. Changes in gamma-tubulin levels can affect the centrosome's function and microtubule dynamics leading to cell cycle dysregulation and tumorigenesis. Moreover it interacts with mutated tumor suppressor proteins like p53 further contributing to cancer progression. Scientists continue to study gamma-tubulin's role in these diseases providing insights into new therapeutic targets.

Product promise

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4 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316)

    ab11316 at a 1/200 dilution staining HeLa cells by Immunocytochemistry. The antibody was incubated with the cells for 30 minutes and then detected using a Cy3 conjugated Goat anti-Mouse (H+L) (green stain). The antibody recognizes gamma-tubulin located at the centrosomes in metaphase and also faintly recognizes the centrosome association during interphase.

    This image is courtesy of an Abreview submitted by Kirk McManus on 2 December 2005.

  • Western blot - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316), expandable thumbnail

    Western blot - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316)

    All lanes: Western blot - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316) at 1/10000 dilution

    Lane 1: HeLa cell line lysates

    Lane 2: U87 cell line lysates

    Lane 3: COS7 cell line lysates

    Lane 4: P19 cell line lysates

    Lane 5: Hepa1-6 cell line lysates

    Lane 6: CHO cell line lysates

    Lane 7: MDBK cell line lysates

    Lane 8: MDCK cell line lysates

    Secondary

    All lanes: Goat Anti-Mouse IgG-Peroxidase

    Predicted band size: 51 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling gamma Tubulin using ab11316 at a dilution of 1/5000. Cells were permeabilized with methanol and folowed by fixation with acetone. A Cy3-conjugated goat anti-mouse IgG was used as the secondary antibody.

  • Western blot - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316), expandable thumbnail

    Western blot - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316)

    Lane 1: Western blot - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316) at 1/20000 dilution

    Lane 2: Negative control (secondary antibody only)

    All lanes: Whole cell extract of chicken fibroblasts

    Secondary

    All lanes: Alkaline Phosphatase conjugated goat anti-mouse IgG

    Predicted band size: 51 kDa

    Observed band size: 48 kDa

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Product protocols

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