AB11316

Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker

Name: Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316) | Abcam
Brand: Abcam Limited
SKU: AB11316
Price: 410 USD
Availability: InStock
Rating: 4 (17 reviews)

Lab Essentials
What is this?

(17 Reviews)

(208 Publications)

Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316) is a mouse monoclonal antibody detecting gamma Tubulin in Western Blot, ICC/IF. Suitable for African green monkey, Chicken, Chinese hamster, Cow, Dog, Human, Mouse.

- Over 170 publications
- Trusted since 2004

View Alternative Names

Tubulin gamma-2 chain, Gamma-2-tubulin, TUBG2

4 Images

Supplier Data

Western blot - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (AB11316)

All lanes:

Western blot - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316) at 1/10000 dilution

Lane 1:

HeLa cell line lysates

Lane 2:

U87 cell line lysates

Lane 3:

COS7 cell line lysates

Lane 4:

P19 cell line lysates

Lane 5:

Hepa1-6 cell line lysates

Lane 6:

CHO cell line lysates

Lane 7:

MDBK cell line lysates

Lane 8:

MDCK cell line lysates

Secondary

All lanes:

Goat Anti-Mouse IgG-Peroxidase

Predicted band size: 51 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (AB11316)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (AB11316)

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling gamma Tubulin using ab11316 at a dilution of 1/5000. Cells were permeabilized with methanol and folowed by fixation with acetone. A Cy3-conjugated goat anti-mouse IgG was used as the secondary antibody.

ICC/IF

AbReview3781****

Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (AB11316)

ab11316 at a 1/200 dilution staining HeLa cells by Immunocytochemistry. The antibody was incubated with the cells for 30 minutes and then detected using a Cy3 conjugated Goat anti-Mouse (H+L) (green stain). The antibody recognizes gamma-tubulin located at the centrosomes in metaphase and also faintly recognizes the centrosome association during interphase.

This image is courtesy of an Abreview submitted by Kirk McManus on 2 December 2005.

Supplier Data

Western blot - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (AB11316)

Lane 1:

Western blot - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316) at 1/20000 dilution

Lane 2:

Negative control (secondary antibody only)

All lanes:

Whole cell extract of chicken fibroblasts

Secondary

All lanes:

Alkaline Phosphatase conjugated goat anti-mouse IgG

Predicted band size: 51 kDa

Observed band size: 48 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

GTU-88

Isotype

IgG1

Carrier free

Reacts with

Mouse, Chicken, Cow, Dog, Human, African green monkey, Chinese hamster

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human TUBG2 aa 1-100 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q9NRH3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "" }, "African green monkey": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "" }, "Chicken": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "" }, "Chinese hamster": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "" }, "Cow": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "" }, "Dog": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF) in African green monkey, Chicken, Chinese hamster, Cow, Dog, Human, Mouse samples.

What is the molecular weight of gamma Tubulin?
Anti-gamma Tubulin [GTU-88] - Centrosome Marker (ab11316) specifically detects a band for gamma Tubulin (UniProt: P23258) at a molecular weight of 48kDa.

Trusted by the scientific community
Anti-gamma Tubulin [GTU-88] - Centrosome Marker (ab11316) was first used in a scientific publication in 2004 and has been cited over 170 times in peer-reviewed journals.

Reviewed by scientists
Anti-gamma Tubulin [GTU-88] - Centrosome Marker (ab11316) has over 15 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purification technique

Proprietary technique

Purification notes

Purified from Tissue Culture Supernatant.

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Gamma-tubulin an important component in the microtubule nucleation process is also known as Gtub or γ-tub. It weighs approximately 48 kDa. Gamma-tubulin is a significant protein inside the centrosome where it plays a role in the formation of microtubule organizing centers. Scientists often find and express it in abundance within rapidly dividing cells both in humans and other eukaryotes. Researchers employ gamma-tubulin staining techniques to visualize its location and understand its function within the centrosome.

Biological function summary

Gamma-tubulin is part of the gamma-tubulin ring complex also known as gamma-TuRC which anchors the microtubule minus ends influencing microtubule stability and organization. This complex functions as a nucleating template for microtubules which are critical for various cell processes including intracellular transport and chromosome separation during cell division. Gamma-tubulin plays an indispensable role in maintaining the correct function of the centrosome an important cellular organelle in cell cycle progression.

Pathways

Gamma-tubulin participates in processes involving cell cycle regulation and mitotic spindle formation. These processes depend heavily on microtubule dynamics and organization. Gamma-tubulin interacts closely with proteins like centrins and pericentrin within these pathways. Such interactions ensure accurate chromosomal segregation during mitosis highlighting its importance in cellular reproduction and stability.

Abnormal gamma-tubulin expression and function relate to conditions like cancer and neurological disorders. Changes in gamma-tubulin levels can affect the centrosome's function and microtubule dynamics leading to cell cycle dysregulation and tumorigenesis. Moreover it interacts with mutated tumor suppressor proteins like p53 further contributing to cancer progression. Scientists continue to study gamma-tubulin's role in these diseases providing insights into new therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tubulin is the major constituent of microtubules, protein filaments consisting of alpha- and beta-tubulin heterodimers (PubMed : 38305685). Gamma-tubulin is a key component of the gamma-tubulin ring complex (gTuRC) which mediates microtubule nucleation (PubMed : 38305685). The gTuRC regulates the minus-end nucleation of alpha-beta tubulin heterodimers that grow into microtubule protafilaments, a critical step in centrosome duplication and spindle formation (PubMed : 38305685).

See full target information TUBG2

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Publications (208)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Nucleic acids 36:102688 PubMed40951761

2025

Molecular treatment options for patients carrying variants.

Applications

Unspecified application

Species

Unspecified reactive species

Jacqueline E Taudien,Sebastian Swirski,Maike Möller,Christoph Jüschke,Marta Owczarek-Lipska,G Christoph Korenke,John Neidhardt

Exploration (Beijing, China) 5:e20230024 PubMed40873651

2025

ASPM Induces Radiotherapy Resistance by Disrupting Microtubule Stability Leading to Chromosome Malsegregation in Non-Small Cell Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Zhong,Ning Liu,Juan Wang,Songbo Xie,Lisheng Liu,Minglei Wang,Fei Wu,Xiaozheng Chen,Changyan Xiao,Xiaoxiao Gongye,Meng Wu,Liewei Wen,Jinming Yu,Dawei Chen

Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association 28:760-775 PubMed40366509

2025

The RNA-binding protein YTHDF3 affects gastric cancer cell migration and response to paclitaxel by regulating EZRIN.

Applications

Unspecified application

Species

Unspecified reactive species

Patrícia Mesquita,Alexandre Coelho,Ana S Ribeiro,Luís F C Póvoas,Catarina de Oliveira,Nelson Leça,Sara Silva,Diana Ferreira,Diana Pádua,Ricardo Coelho,Carmen Jerónimo,Joana Paredes,Carlos Conde,Bruno Pereira,Raquel Almeida

Scientific reports 15:9280 PubMed40102598

2025

Centrobin serves as a safeguard to guide timely centriole maturation during the cell cycle.

Applications

Unspecified application

Species

Unspecified reactive species

Dohyong Lee,Sungjin Ryu,Ji Hwa Hea,Globinna Kim,In-Jeoung Baek,Young Hoon Sung,Kunsoo Rhee

Journal of clinical laboratory analysis 39:e70004 PubMed40059580

2025

UFMylation Modulates OFIP Stability and Centrosomal Localization.

Applications

Unspecified application

Species

Unspecified reactive species

Mulin Yang,Zihe Zhao,Jie Di,Dan Dong,Dengwen Li,Jie Ran

Cell reports 44:115264 PubMed40019835

2025

Neural stem cell relay from B1 to B2 cells in the adult mouse ventricular-subventricular zone.

Applications

Unspecified application

Species

Unspecified reactive species

Arantxa Cebrian-Silla,Marcos Assis Nascimento,Walter Mancia,Susana Gonzalez-Granero,Ricardo Romero-Rodriguez,Kirsten Obernier,David M Steffen,Daniel A Lim,Jose Manuel Garcia-Verdugo,Arturo Alvarez-Buylla

Nature communications 16:1833 PubMed39979259

2025

Increasingly efficient chromatin binding of cohesin and CTCF supports chromatin architecture formation during zebrafish embryogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jonas Coßmann,Pavel I Kos,Vassiliki Varamogianni-Mamatsi,Devin S Assenheimer,Tobias A Bischof,Timo Kuhn,Thomas Vomhof,Argyris Papantonis,Luca Giorgetti,J Christof M Gebhardt

iScience 28:111670 PubMed39898050

2025

Pathogenic variants are associated with defects in primary and motile cilia.

Applications

Unspecified application

Species

Unspecified reactive species

Jacqueline E Taudien,Diana Bracht,Heike Olbrich,Sebastian Swirski,Fulvio D'Abrusco,Bert Van der Zwaag,Maike Möller,Thomas Lücke,Norbert Teig,Ulrika Lindberg,Kai Wohlgemuth,Julia Wallmeier,Anja Blanque,Christos Gatsogiannis,Sebastian George,Christoph Jüschke,Marta Owczarek-Lipska,Dorothee Veer,Hester Y Kroes,Enza Maria Valente,G Christoph Korenke,Heymut Omran,John Neidhardt

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2406849 PubMed39556706

2024

Deficiency of FABP7 Triggers Premature Neural Differentiation in Idiopathic Normocephalic Autism Organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Han,Yuanlin He,Yuanhao Wang,Wenzhu Hu,Chu Chu,Lei Huang,Yuan Hong,Lu Han,Xu Zhang,Yao Gao,Yuan Lin,Hongxia Ma,Hongbing Shen,Xiaoyan Ke,Yan Liu,Zhibin Hu

Cell death & disease 15:752 PubMed39419978

2024

PAK6 rescues pathogenic LRRK2-mediated ciliogenesis and centrosomal cohesion defects in a mutation-specific manner.

Applications

Unspecified application

Species

Unspecified reactive species

Lucia Iannotta,Rachel Fasiczka,Giulia Favetta,Yibo Zhao,Elena Giusto,Elena Dall'Ara,Jianning Wei,Franz Y Ho,Claudia Ciriani,Susanna Cogo,Isabella Tessari,Ciro Iaccarino,Maxime Liberelle,Luigi Bubacco,Jean-Marc Taymans,Claudia Manzoni,Arjan Kortholt,Laura Civiero,Sabine Hilfiker,Michael L Lu,Elisa Greggio

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker

Western blot - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (AB11316)

Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (AB11316)

Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (AB11316)

Western blot - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (AB11316)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (208)

Product promise