Mouse Monoclonal Gamma-2-tubulin antibody. Centrosome marker. Suitable for ICC/IF, WB and reacts with Human, Chinese hamster, Mouse, Chicken, Dog, Cow, African green monkey samples. Cited in 178 publications. Immunogen corresponding to Synthetic Peptide within Human TUBG2 aa 1-100 conjugated to Keyhole Limpet Haemocyanin.
IgG1
Mouse
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS
Liquid
Monoclonal
ICC/IF | WB | |
---|---|---|
Human | Tested | Tested |
Mouse | Expected | Tested |
African green monkey | Expected | Tested |
Chicken | Expected | Tested |
Chinese hamster | Expected | Tested |
Cow | Expected | Tested |
Dog | Expected | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chinese hamster, Mouse, Chicken, Dog, Cow, African green monkey | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chinese hamster | Dilution info 1/10000 | Notes - |
Species Mouse | Dilution info 1/10000 | Notes - |
Species African green monkey | Dilution info 1/10000 | Notes - |
Species Chicken | Dilution info 1/10000 | Notes - |
Species Human | Dilution info 1/10000 | Notes - |
Species Dog | Dilution info 1/10000 | Notes - |
Species Cow | Dilution info 1/10000 | Notes - |
Select an associated product type
Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation (By similarity).
Tubulin gamma-2 chain, Gamma-2-tubulin, TUBG2
Mouse Monoclonal Gamma-2-tubulin antibody. Centrosome marker. Suitable for ICC/IF, WB and reacts with Human, Chinese hamster, Mouse, Chicken, Dog, Cow, African green monkey samples. Cited in 178 publications. Immunogen corresponding to Synthetic Peptide within Human TUBG2 aa 1-100 conjugated to Keyhole Limpet Haemocyanin.
IgG1
Mouse
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS
Liquid
Monoclonal
GTU-88
Proprietary technique
Purified from Tissue Culture Supernatant.
Blue Ice
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This product was changed from ascites to tissue culture supernatant on 18th October 2016. The following lots are from ascites and are still in stock as of 18th October 2016 (GR286332 and GR225007). Lot numbers higher than GR286332 will be from tissue culture supernatant. Please note that dilutions may need to be adjusted accordingly.
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This supplementary information is collated from multiple sources and compiled automatically.
Gamma-tubulin an important component in the microtubule nucleation process is also known as Gtub or γ-tub. It weighs approximately 48 kDa. Gamma-tubulin is a significant protein inside the centrosome where it plays a role in the formation of microtubule organizing centers. Scientists often find and express it in abundance within rapidly dividing cells both in humans and other eukaryotes. Researchers employ gamma-tubulin staining techniques to visualize its location and understand its function within the centrosome.
Gamma-tubulin is part of the gamma-tubulin ring complex also known as gamma-TuRC which anchors the microtubule minus ends influencing microtubule stability and organization. This complex functions as a nucleating template for microtubules which are critical for various cell processes including intracellular transport and chromosome separation during cell division. Gamma-tubulin plays an indispensable role in maintaining the correct function of the centrosome an important cellular organelle in cell cycle progression.
Gamma-tubulin participates in processes involving cell cycle regulation and mitotic spindle formation. These processes depend heavily on microtubule dynamics and organization. Gamma-tubulin interacts closely with proteins like centrins and pericentrin within these pathways. Such interactions ensure accurate chromosomal segregation during mitosis highlighting its importance in cellular reproduction and stability.
Abnormal gamma-tubulin expression and function relate to conditions like cancer and neurological disorders. Changes in gamma-tubulin levels can affect the centrosome's function and microtubule dynamics leading to cell cycle dysregulation and tumorigenesis. Moreover it interacts with mutated tumor suppressor proteins like p53 further contributing to cancer progression. Scientists continue to study gamma-tubulin's role in these diseases providing insights into new therapeutic targets.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
ab11316 at a 1/200 dilution staining HeLa cells by Immunocytochemistry. The antibody was incubated with the cells for 30 minutes and then detected using a Cy3 conjugated Goat anti-Mouse (H+L) (green stain). The antibody recognizes gamma-tubulin located at the centrosomes in metaphase and also faintly recognizes the centrosome association during interphase.
This image is courtesy of an Abreview submitted by Kirk McManus on 2 December 2005.
All lanes: Western blot - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316) at 1/10000 dilution
Lane 1: HeLa cell line lysates
Lane 2: U87 cell line lysates
Lane 3: COS7 cell line lysates
Lane 4: P19 cell line lysates
Lane 5: Hepa1-6 cell line lysates
Lane 6: CHO cell line lysates
Lane 7: MDBK cell line lysates
Lane 8: MDCK cell line lysates
All lanes: Goat Anti-Mouse IgG-Peroxidase
Predicted band size: 51 kDa
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling gamma Tubulin using ab11316 at a dilution of 1/5000. Cells were permeabilized with methanol and folowed by fixation with acetone. A Cy3-conjugated goat anti-mouse IgG was used as the secondary antibody.
Lane 1: Western blot - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316) at 1/20000 dilution
Lane 2: Negative control (secondary antibody only)
All lanes: Whole cell extract of chicken fibroblasts
All lanes: Alkaline Phosphatase conjugated goat anti-mouse IgG
Predicted band size: 51 kDa
Observed band size: 48 kDa
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com