Skip to main content

Rabbit Recombinant Monoclonal TBG1 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human, Cat, Dog, Mouse, Rat, Drosophila melanogaster, African green monkey samples. Immunogen corresponding to Recombinant Fragment Protein within Human TUBG1.

Be the first to review this product! Submit a review

Images

Western blot - Anti-gamma Tubulin antibody [HL1175] (AB308382), expandable thumbnail
  • Western blot - Anti-gamma Tubulin antibody [HL1175] (AB308382), expandable thumbnail
  • Western blot - Anti-gamma Tubulin antibody [HL1175] (AB308382), expandable thumbnail
  • Western blot - Anti-gamma Tubulin antibody [HL1175] (AB308382), expandable thumbnail
  • Western blot - Anti-gamma Tubulin antibody [HL1175] (AB308382), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human TUBG1. The exact immunogen used to generate this antibody is proprietary information. Database link P23258

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWB
Human
Tested
Tested
Mouse
Expected
Tested
Rat
Expected
Tested
African green monkey
Expected
Tested
Cat
Expected
Tested
Dog
Expected
Tested
Drosophila melanogaster
Expected
Tested

Tested
Tested

Species
Human
Dilution info
1/2500
Notes

-

Expected
Expected

Species
Cat, Dog, Mouse, Rat, Drosophila melanogaster, African green monkey
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Cat
Dilution info
1/500 - 1/3000
Notes

-

Species
Dog
Dilution info
1/500 - 1/3000
Notes

-

Species
Human
Dilution info
1/500 - 1/3000
Notes

-

Species
Mouse
Dilution info
1/500 - 1/3000
Notes

-

Species
Rat
Dilution info
1/500 - 1/3000
Notes

-

Species
Drosophila melanogaster
Dilution info
1/500 - 1/3000
Notes

-

Species
African green monkey
Dilution info
1/500 - 1/3000
Notes

-

Target data

Function

Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation.

Alternative names

TUBG, TUBG1, Tubulin gamma-1 chain, Gamma-1-tubulin, Gamma-tubulin complex component 1, GCP-1

Recommended products

Rabbit Recombinant Monoclonal TBG1 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human, Cat, Dog, Mouse, Rat, Drosophila melanogaster, African green monkey samples. Immunogen corresponding to Recombinant Fragment Protein within Human TUBG1.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogens
  • Recombinant Fragment Protein within Human TUBG1. The exact immunogen used to generate this antibody is proprietary information. Database link P23258
Carrier free
Yes
Clone number
HL1175
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Gamma-tubulin an important component in the microtubule nucleation process is also known as Gtub or γ-tub. It weighs approximately 48 kDa. Gamma-tubulin is a significant protein inside the centrosome where it plays a role in the formation of microtubule organizing centers. Scientists often find and express it in abundance within rapidly dividing cells both in humans and other eukaryotes. Researchers employ gamma-tubulin staining techniques to visualize its location and understand its function within the centrosome.

Biological function summary

Gamma-tubulin is part of the gamma-tubulin ring complex also known as gamma-TuRC which anchors the microtubule minus ends influencing microtubule stability and organization. This complex functions as a nucleating template for microtubules which are critical for various cell processes including intracellular transport and chromosome separation during cell division. Gamma-tubulin plays an indispensable role in maintaining the correct function of the centrosome an important cellular organelle in cell cycle progression.

Pathways

Gamma-tubulin participates in processes involving cell cycle regulation and mitotic spindle formation. These processes depend heavily on microtubule dynamics and organization. Gamma-tubulin interacts closely with proteins like centrins and pericentrin within these pathways. Such interactions ensure accurate chromosomal segregation during mitosis highlighting its importance in cellular reproduction and stability.

Associated diseases and disorders

Abnormal gamma-tubulin expression and function relate to conditions like cancer and neurological disorders. Changes in gamma-tubulin levels can affect the centrosome's function and microtubule dynamics leading to cell cycle dysregulation and tumorigenesis. Moreover it interacts with mutated tumor suppressor proteins like p53 further contributing to cancer progression. Scientists continue to study gamma-tubulin's role in these diseases providing insights into new therapeutic targets.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-gamma Tubulin antibody [HL1175] (ab308382), expandable thumbnail

    Western blot - Anti-gamma Tubulin antibody [HL1175] (ab308382)

    Samples were seperated by 10% SDS-PAGE.

    All lanes: Western blot - Anti-gamma Tubulin antibody [HL1175] (ab308382) at 1/1000 dilution

    Lane 1: Vero whole cell extracts at 30 µg

    Lane 2: CHO whole cell extracts at 30 µg

    Lane 3: RK 13 whole cell extracts at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

  • Western blot - Anti-gamma Tubulin antibody [HL1175] (ab308382), expandable thumbnail

    Western blot - Anti-gamma Tubulin antibody [HL1175] (ab308382)

    All lanes: Western blot - Anti-gamma Tubulin antibody [HL1175] (ab308382) at 1/1000 dilution

    All lanes: Whole S2 cell extract at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 51 kDa

  • Western blot - Anti-gamma Tubulin antibody [HL1175] (ab308382), expandable thumbnail

    Western blot - Anti-gamma Tubulin antibody [HL1175] (ab308382)

    All lanes: Western blot - Anti-gamma Tubulin antibody [HL1175] (ab308382) at 1/3000 dilution

    All lanes: HEK293T cell lysate at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 51.1 kDa

  • Western blot - Anti-gamma Tubulin antibody [HL1175] (ab308382), expandable thumbnail

    Western blot - Anti-gamma Tubulin antibody [HL1175] (ab308382)

    For immunofluorescence analysis, HeLa cells were fixed in ice-cold MeOH for 5 minutes and permeabilized for detection of gamma Tubulin protein using ab308382 at 1/2500 dilution (green). Nuclei (blue) is stained with fluoroshield with DAPI.

  • Western blot - Anti-gamma Tubulin antibody [HL1175] (ab308382), expandable thumbnail

    Western blot - Anti-gamma Tubulin antibody [HL1175] (ab308382)

    Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with gamma Tubulin antibody [HL1175] (ab308382) diluted at 1/5000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

    Lanes 1 - 2: Western blot - Anti-gamma Tubulin antibody [HL1175] (ab308382) at 1/5000 dilution

    Lane 1: MDCK cell lysate at 30 µg

    Lane 2: PG4 cell lysate at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 51.1 kDa

  • Western blot - Anti-gamma Tubulin antibody [HL1175] (ab308382), expandable thumbnail

    Western blot - Anti-gamma Tubulin antibody [HL1175] (ab308382)

    Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with gamma Tubulin antibody [HL1175] (ab308382) diluted at 1/10000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

    All lanes: Western blot - Anti-gamma Tubulin antibody [HL1175] (ab308382) at 1/10000 dilution

    Lane 1: Neuro2A cell lysate at 30 µg

    Lane 2: Rat2 cell lysate at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 51.1 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [HL1175] (ab308382), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [HL1175] (ab308382)

    For immunofluorescence analysis, HeLa cells were fixed in ice-cold MeOH for 5 minutes and permeabilized for detection of gamma Tubulin protein using ab308382 at 1/2500 dilution (green). Nuclei (blue) is stained with fluoroshield with DAPI. Alpha Tubulin stained by alpha Tubulin antibody (red) diluted at 1/1000

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com