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AB308382

Anti-gamma Tubulin antibody [HL1175] - Centrosome Marker

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Rabbit Recombinant Monoclonal TBG1 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human, Cat, Dog, Mouse, Rat, Drosophila melanogaster, African green monkey samples. Immunogen corresponding to Recombinant Fragment Protein within Human TUBG1.

View Alternative Names

TUBG, TUBG1, Tubulin gamma-1 chain, Gamma-1-tubulin, Gamma-tubulin complex component 1, GCP-1

7 Images
Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [HL1175] - Centrosome Marker (AB308382)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [HL1175] - Centrosome Marker (AB308382)

For immunofluorescence analysis, HeLa cells were fixed in ice-cold MeOH for 5 minutes and permeabilized for detection of gamma Tubulin protein using ab308382 at 1/2500 dilution (green). Nuclei (blue) is stained with fluoroshield with DAPI. Alpha Tubulin stained by alpha Tubulin antibody (red) diluted at 1/1000

Western blot - Anti-gamma Tubulin antibody [HL1175] - Centrosome Marker (AB308382)
  • WB

Supplier Data

Western blot - Anti-gamma Tubulin antibody [HL1175] - Centrosome Marker (AB308382)

For immunofluorescence analysis, HeLa cells were fixed in ice-cold MeOH for 5 minutes and permeabilized for detection of gamma Tubulin protein using ab308382 at 1/2500 dilution (green). Nuclei (blue) is stained with fluoroshield with DAPI.

Western blot - Anti-gamma Tubulin antibody [HL1175] - Centrosome Marker (AB308382)
  • WB

Supplier Data

Western blot - Anti-gamma Tubulin antibody [HL1175] - Centrosome Marker (AB308382)

All lanes:

Western blot - Anti-gamma Tubulin antibody [HL1175] - Centrosome Marker (ab308382) at 1/3000 dilution

All lanes:

HEK293T cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 51.1 kDa

false

Western blot - Anti-gamma Tubulin antibody [HL1175] - Centrosome Marker (AB308382)
  • WB

Supplier Data

Western blot - Anti-gamma Tubulin antibody [HL1175] - Centrosome Marker (AB308382)

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with gamma Tubulin antibody [HL1175] (ab308382) diluted at 1/10000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-gamma Tubulin antibody [HL1175] - Centrosome Marker (ab308382) at 1/10000 dilution

Lane 1:

Neuro2A cell lysate at 30 µg

Lane 2:

Rat2 cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 51.1 kDa

false

Western blot - Anti-gamma Tubulin antibody [HL1175] - Centrosome Marker (AB308382)
  • WB

Supplier Data

Western blot - Anti-gamma Tubulin antibody [HL1175] - Centrosome Marker (AB308382)

All lanes:

Western blot - Anti-gamma Tubulin antibody [HL1175] - Centrosome Marker (ab308382) at 1/1000 dilution

All lanes:

Whole S2 cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 51 kDa

false

Western blot - Anti-gamma Tubulin antibody [HL1175] - Centrosome Marker (AB308382)
  • WB

Supplier Data

Western blot - Anti-gamma Tubulin antibody [HL1175] - Centrosome Marker (AB308382)

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with gamma Tubulin antibody [HL1175] (ab308382) diluted at 1/5000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Lanes 1 - 2:

Western blot - Anti-gamma Tubulin antibody [HL1175] - Centrosome Marker (ab308382) at 1/5000 dilution

Lane 1:

MDCK cell lysate at 30 µg

Lane 2:

PG4 cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 51.1 kDa

false

Western blot - Anti-gamma Tubulin antibody [HL1175] - Centrosome Marker (AB308382)
  • WB

Supplier Data

Western blot - Anti-gamma Tubulin antibody [HL1175] - Centrosome Marker (AB308382)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-gamma Tubulin antibody [HL1175] - Centrosome Marker (ab308382) at 1/1000 dilution

Lane 1:

Vero whole cell extracts at 30 µg

Lane 2:

CHO whole cell extracts at 30 µg

Lane 3:

RK 13 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1175

Isotype

IgG

Carrier free

Yes

Reacts with

Cat, Dog, Human, Mouse, Rat, Drosophila melanogaster, African green monkey

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Recombinant Fragment Protein within Human TUBG2. Database link Q9NRH3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/2500", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "African green monkey": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Cat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Dog": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Drosophila melanogaster": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Gamma-tubulin an important component in the microtubule nucleation process is also known as Gtub or γ-tub. It weighs approximately 48 kDa. Gamma-tubulin is a significant protein inside the centrosome where it plays a role in the formation of microtubule organizing centers. Scientists often find and express it in abundance within rapidly dividing cells both in humans and other eukaryotes. Researchers employ gamma-tubulin staining techniques to visualize its location and understand its function within the centrosome.
Biological function summary

Gamma-tubulin is part of the gamma-tubulin ring complex also known as gamma-TuRC which anchors the microtubule minus ends influencing microtubule stability and organization. This complex functions as a nucleating template for microtubules which are critical for various cell processes including intracellular transport and chromosome separation during cell division. Gamma-tubulin plays an indispensable role in maintaining the correct function of the centrosome an important cellular organelle in cell cycle progression.

Pathways

Gamma-tubulin participates in processes involving cell cycle regulation and mitotic spindle formation. These processes depend heavily on microtubule dynamics and organization. Gamma-tubulin interacts closely with proteins like centrins and pericentrin within these pathways. Such interactions ensure accurate chromosomal segregation during mitosis highlighting its importance in cellular reproduction and stability.

Abnormal gamma-tubulin expression and function relate to conditions like cancer and neurological disorders. Changes in gamma-tubulin levels can affect the centrosome's function and microtubule dynamics leading to cell cycle dysregulation and tumorigenesis. Moreover it interacts with mutated tumor suppressor proteins like p53 further contributing to cancer progression. Scientists continue to study gamma-tubulin's role in these diseases providing insights into new therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation.
See full target information TUBG1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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