Mouse Monoclonal Gamma-2-tubulin antibody. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 59 publications. Immunogen corresponding to Synthetic Peptide within Human TUBG1 aa 400-450 conjugated to Keyhole Limpet Haemocyanin.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (AB27074), expandable thumbnail](https://content.abcam.com/products/images/gamma-tubulin-antibody-tu-30-centrosome-marker-ab27074--immunocytochemistry-immunofluorescence-img103457.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (AB27074)")
![Flow Cytometry (Intracellular) - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (AB27074), expandable thumbnail](https://content.abcam.com/products/images/gamma-tubulin-antibody-tu-30-centrosome-marker-ab27074--flow-cytometry-intracellular-img78036.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (AB27074)")
![Western blot - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (AB27074), expandable thumbnail](https://content.abcam.com/products/images/gamma-tubulin-antibody-tu-30-centrosome-marker-ab27074--western-blot-img178849.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (AB27074)")
![Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (AB27074), expandable thumbnail](https://content.abcam.com/products/images/gamma-tubulin-antibody-tu-30-centrosome-marker-ab27074--immunocytochemistry-immunofluorescence-img64334.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (AB27074)")
![Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (AB27074), expandable thumbnail](https://content.abcam.com/products/images/gamma-tubulin-antibody-tu-30-centrosome-marker-ab27074--immunocytochemistry-immunofluorescence-img94760.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (AB27074)")
Publications
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- Nature communications 14:17152023Distinct dynein complexes defined by DYNLRB1 and DYNLRB2 regulate mitotic and male meiotic spindle bipolarity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuwen He,John P Gillies,Juliana L Zang,Carmen M Córdoba-Beldad,Io Yamamoto,Yasuhiro Fujiwara,Julie Grantham,Morgan E DeSantis,Hiroki ShibuyaPubMed 36973253
- Oncogene 42:572-5852022BAP1 loss induces mitotic defects in mesothelioma cells through BRCA1-dependent and independent mechanisms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnita Singh,Sara Busacca,Aarti Gaba,Michael Sheaff,Charlotte Poile,Apostolos Nakas,Joanna Dzialo,Aleksandra Bzura,Alan G Dawson,Dean A Fennell,Andrew M FryPubMed 36550359
- The Journal of cell biology 221:2022The central scaffold protein CEP350 coordinates centriole length, stability, and maturation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOnur Rojhat Karasu,Annett Neuner,Enrico Salvatore Atorino,Gislene Pereira,Elmar SchiebelPubMed 36315013
- PloS one 17:e02688482022A commercial ARHGEF17/TEM4 antibody cross-reacts with Nuclear Mitotic Apparatus protein 1 (NuMA).Applications:Unspecified applicationReactive species:Unspecified reactive speciesDiogjena Katerina Prifti,Annie Lauzier,Sabine ElowePubMed 35776709
- EMBO reports 23:e538052022Centrosome linker protein C-Nap1 maintains stem cells in mouse testes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHairuo Dang,Ana Martin-Villalba,Elmar SchiebelPubMed 35599622
- The Prostate 82:957-9692022PLK4 is upregulated in prostate cancer and its inhibition reduces centrosome amplification and causes senescence.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChandra K Singh,Ryan A Denu,Minakshi Nihal,Maria Shabbir,Debra R Garvey,Wei Huang,Kenneth A Iczkowski,Nihal AhmadPubMed 35333404
- Nature communications 13:6682022Cyclin B/CDK1 and Cyclin A/CDK2 phosphorylate DENR to promote mitotic protein translation and faithful cell division.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatharina Clemm von Hohenberg,Sandra Müller,Sibylle Schleich,Matthias Meister,Jonathan Bohlen,Thomas G Hofmann,Aurelio A TelemanPubMed 35115540
- Nature communications 13:4732022Modular assembly of the principal microtubule nucleator γ-TuRC.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMartin Würtz,Erik Zupa,Enrico S Atorino,Annett Neuner,Anna Böhler,Ariani S Rahadian,Bram J A Vermeulen,Giulia Tonon,Sebastian Eustermann,Elmar Schiebel,Stefan PfefferPubMed 35078983
- ACS nano 15:17528-175482021Mechanical Counterbalance of Kinesin and Dynein Motors in a Microtubular Network Regulates Cell Mechanics, 3D Architecture, and Mechanosensing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexander S Zhovmer,Alexis Manning,Chynna Smith,James B Hayes,Dylan T Burnette,Jian Wang,Alexander X Cartagena-Rivera,Nikolay V Dokholyan,Rakesh K Singh,Erdem D TabdanovPubMed 34677937
- Journal of virology 95:e00821212021Human Cytomegalovirus Exploits TACC3 To Control Microtubule Dynamics and Late Stages of Infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesColleen Furey,Helen Astar,Derek WalshPubMed 34191581
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Synthetic Peptide within Human TUBG1 aa 400-450 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P23258
Reactivity data
Select an application| ICC/IF | WB | Flow Cyt (Intra) | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
| Rat | Tested | Expected | Expected |
| Chicken | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
| Plants | Predicted | Predicted | Predicted |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes Note: Fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C. Alternatively, fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. After fixing cells, incubate for 45 minutes at room temperature. This antibody will only stain fixed cells. |
Species Mouse | Dilution info 1.00000-2.00000 µg/mL | Notes Note: Fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C. Alternatively, fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. After fixing cells, incubate for 45 minutes at room temperature. This antibody will only stain fixed cells. |
Species Rat | Dilution info 1.00000-2.00000 µg/mL | Notes Note: Fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C. Alternatively, fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. After fixing cells, incubate for 45 minutes at room temperature. This antibody will only stain fixed cells. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Pig, Plants | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes reducing conditions. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1.00000-2.00000 µg/mL | Notes reducing conditions. |
Species Rat | Dilution info 1.00000-2.00000 µg/mL | Notes reducing conditions. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Pig, Plants | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg for 106 Cells | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Pig, Plants | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation (By similarity).
Alternative names
Tubulin gamma-2 chain, Gamma-2-tubulin, TUBG2
Recommended products
Mouse Monoclonal Gamma-2-tubulin antibody. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 59 publications. Immunogen corresponding to Synthetic Peptide within Human TUBG1 aa 400-450 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Synthetic Peptide within Human TUBG1 aa 400-450 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P23258
- Clone number
- TU-30
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody recognises gamma tubulin expressed on stomach, kidney, liver, lung, muscle, ovary, heart, colon, thymus, brain.
- Epitope
- This antibody reacts with an epitope located in the C terminal region of gamma tubulin.
- Concentration
- Purification notes
This antibody was purified from hybridoma culture supernatant by affinity chromatography. Purity is > 95% (by SDS-PAGE).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Gamma-tubulin an important component in the microtubule nucleation process is also known as Gtub or γ-tub. It weighs approximately 48 kDa. Gamma-tubulin is a significant protein inside the centrosome where it plays a role in the formation of microtubule organizing centers. Scientists often find and express it in abundance within rapidly dividing cells both in humans and other eukaryotes. Researchers employ gamma-tubulin staining techniques to visualize its location and understand its function within the centrosome.
- Biological function summary
Gamma-tubulin is part of the gamma-tubulin ring complex also known as gamma-TuRC which anchors the microtubule minus ends influencing microtubule stability and organization. This complex functions as a nucleating template for microtubules which are critical for various cell processes including intracellular transport and chromosome separation during cell division. Gamma-tubulin plays an indispensable role in maintaining the correct function of the centrosome an important cellular organelle in cell cycle progression.
- Pathways
Gamma-tubulin participates in processes involving cell cycle regulation and mitotic spindle formation. These processes depend heavily on microtubule dynamics and organization. Gamma-tubulin interacts closely with proteins like centrins and pericentrin within these pathways. Such interactions ensure accurate chromosomal segregation during mitosis highlighting its importance in cellular reproduction and stability.
- Associated diseases and disorders
Abnormal gamma-tubulin expression and function relate to conditions like cancer and neurological disorders. Changes in gamma-tubulin levels can affect the centrosome's function and microtubule dynamics leading to cell cycle dysregulation and tumorigenesis. Moreover it interacts with mutated tumor suppressor proteins like p53 further contributing to cancer progression. Scientists continue to study gamma-tubulin's role in these diseases providing insights into new therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (ab27074), expandable thumbnail](https://content.abcam.com/products/images/gamma-tubulin-antibody-tu-30-centrosome-marker-ab27074--immunocytochemistry-immunofluorescence-img103457.jpg "Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (ab27074)")
Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (ab27074)
Gamma Tubulin Immunocytochemistry/ Immunofluorescence staining using mouse Anti-gamma Tubulin antibody
Immunocytochemistry staining of P19X1 mouse embryonal carcinoma cell line using anti-gamma-tubulin (TU-30) (detection by secondary antibody Goat anti-mouse Cy3). Nuclei were stained with DAPI (blue).
![Flow Cytometry (Intracellular) - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (ab27074), expandable thumbnail](https://content.abcam.com/products/images/gamma-tubulin-antibody-tu-30-centrosome-marker-ab27074--flow-cytometry-intracellular-img78036.jpg "Flow Cytometry (Intracellular) - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (ab27074)")
Flow Cytometry (Intracellular) - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (ab27074)
Gamma Tubulin Flow Cytometry (Intracellular) staining using mouse Anti-gamma Tubulin antibody
Overlay histogram showing HeLa cells stained with ab27074 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab27074, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
![Western blot - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (ab27074), expandable thumbnail](https://content.abcam.com/products/images/gamma-tubulin-antibody-tu-30-centrosome-marker-ab27074--western-blot-img178849.jpg "Western blot - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (ab27074)")
Western blot - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (ab27074)
Gamma Tubulin Western blot staining using mouse Anti-gamma Tubulin antibody
Western blotting analysis of gamma-tubulin using ab27074 on lysates of various cell lines under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 μg/ml of ab27074 followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for gamma-tubulin at approximately 46 kDa.
All lanes: Western blot - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (ab27074)
Predicted band size: 51 kDa
Observed band size: 46 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (ab27074), expandable thumbnail](https://content.abcam.com/products/images/gamma-tubulin-antibody-tu-30-centrosome-marker-ab27074--immunocytochemistry-immunofluorescence-img64334.jpg "Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (ab27074)")
Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (ab27074)
Gamma Tubulin Immunocytochemistry/ Immunofluorescence staining using mouse Anti-gamma Tubulin antibody
Immunocytochemisrty analysis of murine fibroblasts labeling gamma Tubulin with ab27074 at 1 ug/ml (red). Nuclei were stained with DAPI (blue).
![Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (ab27074), expandable thumbnail](https://content.abcam.com/products/images/gamma-tubulin-antibody-tu-30-centrosome-marker-ab27074--immunocytochemistry-immunofluorescence-img94760.jpg "Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (ab27074)")
Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (ab27074)
Gamma Tubulin Immunocytochemistry/ Immunofluorescence staining using mouse Anti-gamma Tubulin antibody
Immunocytochemisrty analysis of 3T3 mouse fibroblasts in metaphase (A), anaphase (B) and telophase (C), labeling gamma Tubulin with ab27074 at 1 ug/ml (red). Cells were costained for alpha-tubulin (green). Nuclei were stained with DAPI (blue).
![Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (ab27074), expandable thumbnail](https://content.abcam.com/products/images/gamma-tubulin-antibody-tu-30-centrosome-marker-ab27074--immunocytochemistry-immunofluorescence-img139134.jpg "Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (ab27074)")
This image is courtesy of an anonymous customer reviewImmunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (ab27074)
Gamma Tubulin Immunocytochemistry/ Immunofluorescence staining using mouse Anti-gamma Tubulin antibody
ab27074 staining gamma tubulin in the Human HEK293T cells by ICC. Cells were fixed with paraformaldehyde, permeabilized with methanol (-20°C 20 min) and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/500 or 1/1000 in blocking solution) for 45 minutes. A Texas Red®-conjugated Goat anti-mouse polyclonal (1/500) was used as the secondary antibody.
![Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (ab27074), expandable thumbnail](https://content.abcam.com/products/images/gamma-tubulin-antibody-tu-30-centrosome-marker-ab27074--immunocytochemistry-immunofluorescence-img21649.jpg "Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (ab27074)")
Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (ab27074)
Gamma Tubulin Immunocytochemistry/ Immunofluorescence staining using mouse Anti-gamma Tubulin antibody
ab27074 staining gamma tubulin (red) in rat prostate carcinoma cells by ICC. The cells were also transfected with beta-tubulin GFP which stained green.
![Flow Cytometry (Intracellular) - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (ab27074), expandable thumbnail](https://content.abcam.com/products/images/gamma-tubulin-antibody-tu-30-centrosome-marker-ab27074--flow-cytometry-intracellular-img418798.jpg "Flow Cytometry (Intracellular) - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (ab27074)")
Flow Cytometry (Intracellular) - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (ab27074)
Gamma Tubulin Flow Cytometry (Intracellular) staining using mouse Anti-gamma Tubulin antibody
Separation of MCF-7 cells stained using ab27074 (concentration in sample 9 μg/ml, GAM APC, red-filled) from MCF-7 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).
![Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (ab27074), expandable thumbnail](https://content.abcam.com/products/images/gamma-tubulin-antibody-tu-30-centrosome-marker-ab27074--immunocytochemistry-immunofluorescence-img139134.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (ab27074)")
![Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (ab27074), expandable thumbnail](https://content.abcam.com/products/images/gamma-tubulin-antibody-tu-30-centrosome-marker-ab27074--immunocytochemistry-immunofluorescence-img21649.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (ab27074)")
![Flow Cytometry (Intracellular) - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (ab27074), expandable thumbnail](https://content.abcam.com/products/images/gamma-tubulin-antibody-tu-30-centrosome-marker-ab27074--flow-cytometry-intracellular-img418798.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (ab27074)")
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