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Mouse Monoclonal Gamma-2-tubulin antibody. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 59 publications. Immunogen corresponding to Synthetic Peptide within Human TUBG1 aa 400-450 conjugated to Keyhole Limpet Haemocyanin.


Images

Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (AB27074), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (AB27074), expandable thumbnail
  • Western blot - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (AB27074), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (AB27074), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (AB27074), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.4
Preservative: 0.097% Sodium azide

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Synthetic Peptide within Human TUBG1 aa 400-450 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P23258

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWBFlow Cyt (Intra)
Human
Tested
Tested
Tested
Mouse
Tested
Expected
Expected
Rat
Tested
Expected
Expected
Chicken
Predicted
Predicted
Predicted
Cow
Predicted
Predicted
Predicted
Pig
Predicted
Predicted
Predicted
Plants
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1.00000-2.00000 µg/mL
Notes

Note: Fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C. Alternatively, fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. After fixing cells, incubate for 45 minutes at room temperature. This antibody will only stain fixed cells.

Species
Mouse
Dilution info
1.00000-2.00000 µg/mL
Notes

Note: Fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C. Alternatively, fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. After fixing cells, incubate for 45 minutes at room temperature. This antibody will only stain fixed cells.

Species
Rat
Dilution info
1.00000-2.00000 µg/mL
Notes

Note: Fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C. Alternatively, fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. After fixing cells, incubate for 45 minutes at room temperature. This antibody will only stain fixed cells.

Predicted
Predicted

Species
Chicken, Cow, Pig, Plants
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1.00000-2.00000 µg/mL
Notes

reducing conditions.

Expected
Expected

Species
Mouse
Dilution info
1.00000-2.00000 µg/mL
Notes

reducing conditions.

Species
Rat
Dilution info
1.00000-2.00000 µg/mL
Notes

reducing conditions.

Predicted
Predicted

Species
Chicken, Cow, Pig, Plants
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1 µg for 106 Cells
Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Chicken, Cow, Pig, Plants
Dilution info
-
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation (By similarity).

Alternative names

Recommended products

Mouse Monoclonal Gamma-2-tubulin antibody. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 59 publications. Immunogen corresponding to Synthetic Peptide within Human TUBG1 aa 400-450 conjugated to Keyhole Limpet Haemocyanin.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Synthetic Peptide within Human TUBG1 aa 400-450 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P23258
Clone number
TU-30
Purification technique
Affinity purification Protein A
Specificity

This antibody recognises gamma tubulin expressed on stomach, kidney, liver, lung, muscle, ovary, heart, colon, thymus, brain.

Epitope
This antibody reacts with an epitope located in the C terminal region of gamma tubulin.
Concentration
Loading...
Purification notes

This antibody was purified from hybridoma culture supernatant by affinity chromatography. Purity is > 95% (by SDS-PAGE).

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Gamma-tubulin an important component in the microtubule nucleation process is also known as Gtub or γ-tub. It weighs approximately 48 kDa. Gamma-tubulin is a significant protein inside the centrosome where it plays a role in the formation of microtubule organizing centers. Scientists often find and express it in abundance within rapidly dividing cells both in humans and other eukaryotes. Researchers employ gamma-tubulin staining techniques to visualize its location and understand its function within the centrosome.

Biological function summary

Gamma-tubulin is part of the gamma-tubulin ring complex also known as gamma-TuRC which anchors the microtubule minus ends influencing microtubule stability and organization. This complex functions as a nucleating template for microtubules which are critical for various cell processes including intracellular transport and chromosome separation during cell division. Gamma-tubulin plays an indispensable role in maintaining the correct function of the centrosome an important cellular organelle in cell cycle progression.

Pathways

Gamma-tubulin participates in processes involving cell cycle regulation and mitotic spindle formation. These processes depend heavily on microtubule dynamics and organization. Gamma-tubulin interacts closely with proteins like centrins and pericentrin within these pathways. Such interactions ensure accurate chromosomal segregation during mitosis highlighting its importance in cellular reproduction and stability.

Associated diseases and disorders

Abnormal gamma-tubulin expression and function relate to conditions like cancer and neurological disorders. Changes in gamma-tubulin levels can affect the centrosome's function and microtubule dynamics leading to cell cycle dysregulation and tumorigenesis. Moreover it interacts with mutated tumor suppressor proteins like p53 further contributing to cancer progression. Scientists continue to study gamma-tubulin's role in these diseases providing insights into new therapeutic targets.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (ab27074), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (ab27074)

    Gamma Tubulin Immunocytochemistry/ Immunofluorescence staining using mouse Anti-gamma Tubulin antibody

    Immunocytochemistry staining of P19X1 mouse embryonal carcinoma cell line using anti-gamma-tubulin (TU-30) (detection by secondary antibody Goat anti-mouse Cy3). Nuclei were stained with DAPI (blue).

  • Flow Cytometry (Intracellular) - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (ab27074), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (ab27074)

    Gamma Tubulin Flow Cytometry (Intracellular) staining using mouse Anti-gamma Tubulin antibody

    Overlay histogram showing HeLa cells stained with ab27074 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab27074, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Western blot - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (ab27074), expandable thumbnail

    Western blot - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (ab27074)

    Gamma Tubulin Western blot staining using mouse Anti-gamma Tubulin antibody

    Western blotting analysis of gamma-tubulin using ab27074 on lysates of various cell lines under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 μg/ml of ab27074 followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for gamma-tubulin at approximately 46 kDa.

    All lanes: Western blot - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (ab27074)

    Predicted band size: 51 kDa

    Observed band size: 46 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (ab27074), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (ab27074)

    Gamma Tubulin Immunocytochemistry/ Immunofluorescence staining using mouse Anti-gamma Tubulin antibody

    Immunocytochemisrty analysis of murine fibroblasts labeling gamma Tubulin with ab27074 at 1 ug/ml (red). Nuclei were stained with DAPI (blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (ab27074), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (ab27074)

    Gamma Tubulin Immunocytochemistry/ Immunofluorescence staining using mouse Anti-gamma Tubulin antibody

    Immunocytochemisrty analysis of 3T3 mouse fibroblasts in metaphase (A), anaphase (B) and telophase (C), labeling gamma Tubulin with ab27074 at 1 ug/ml (red). Cells were costained for alpha-tubulin (green). Nuclei were stained with DAPI (blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (ab27074), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (ab27074)

    Gamma Tubulin Immunocytochemistry/ Immunofluorescence staining using mouse Anti-gamma Tubulin antibody

    ab27074 staining gamma tubulin in the Human HEK293T cells by ICC. Cells were fixed with paraformaldehyde, permeabilized with methanol (-20°C 20 min) and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/500 or 1/1000 in blocking solution) for 45 minutes. A Texas Red®-conjugated Goat anti-mouse polyclonal (1/500) was used as the secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (ab27074), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (ab27074)

    Gamma Tubulin Immunocytochemistry/ Immunofluorescence staining using mouse Anti-gamma Tubulin antibody

    ab27074 staining gamma tubulin (red) in rat prostate carcinoma cells by ICC. The cells were also transfected with beta-tubulin GFP which stained green.

  • Flow Cytometry (Intracellular) - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (ab27074), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker (ab27074)

    Gamma Tubulin Flow Cytometry (Intracellular) staining using mouse Anti-gamma Tubulin antibody

    Separation of MCF-7 cells stained using ab27074 (concentration in sample 9 μg/ml, GAM APC, red-filled) from MCF-7 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).

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Product protocols

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