Rabbit Polyclonal GAMT antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Guanidinoacetate N-methyltransferase.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Mouse Guanidinoacetate N-methyltransferase. Database link O35969
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Mouse | Tested | Tested |
| Rat | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.50000-2.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 5.00000-20.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Converts guanidinoacetate to creatine, using S-adenosylmethionine as the methyl donor. Important in nervous system development.
Alternative names
Guanidinoacetate N-methyltransferase, Gamt
Recommended products
Rabbit Polyclonal GAMT antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Guanidinoacetate N-methyltransferase.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Mouse Guanidinoacetate N-methyltransferase. Database link O35969
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab231656 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Guanidinoacetate N-methyltransferase commonly known as GAMT catalyzes the conversion of guanidinoacetate to creatine through methylation. The enzyme has a molecular mass of approximately 24 kDa. GAMT is expressed in various body tissues with high levels observed in the liver and kidney. The enzyme plays an important role in the synthesis of creatine an important molecule for energy storage and supply in muscle tissues.
- Biological function summary
GAMT participates in the final step of creatine biosynthesis. This enzyme acts independently rather than forming part of a larger complex. In the cells creatine serves as an energy buffer predominantly involved in tissues with high and fluctuating energy demands such as muscles and the brain. GAMT's activity ensures a steady supply of creatine which supports cellular energy homeostasis.
- Pathways
GAMT plays a significant role in the creatine biosynthesis pathway. This pathway is vital for maintaining the energy balance in cells especially those with a high-energy requirement. GAMT directly interacts with guanidinoacetate as a substrate with S-adenosylmethionine serving as the methyl group donor. Additionally GAMT is connected to the arginine and proline metabolism pathway alongside other enzymes such as arginine-glycine amidinotransferase (AGAT) which acts earlier in the creatine biosynthetic pathway.
- Associated diseases and disorders
Mutations or deficiencies in GAMT associate with disorders such as GAMT deficiency which leads to creatine deficiency syndromes. This condition typically results in neurological impairments including developmental delays and cognitive dysfunctions. The disorder also relates to disrupted creatine production in which AGAT and other enzymes within the creatine biosynthetic pathway may contribute to similar phenotypic outcomes. Research into these connections aids in understanding the broader implications of GAMT dysfunction.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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10 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAMT antibody (ab231656)
Paraffin-embedded mouse uterus tissue stained for GAMT using ab231656 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAMT antibody (ab231656)
Paraffin-embedded mouse testis tissue stained for GAMT using ab231656 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAMT antibody (ab231656)
Paraffin-embedded mouse liver tissue stained for GAMT using ab231656 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAMT antibody (ab231656)
Paraffin-embedded mouse brain tissue stained for GAMT using ab231656 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAMT antibody (ab231656)
Paraffin-embedded mouse uterus tissue stained for GAMT using ab231656 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAMT antibody (ab231656)
Paraffin-embedded mouse testis tissue stained for GAMT using ab231656 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAMT antibody (ab231656)
Paraffin-embedded mouse liver tissue stained for GAMT using ab231656 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAMT antibody (ab231656)
Paraffin-embedded mouse stomach tissue stained for GAMT using ab231656 at 10 μg/ml in immunohistochemical analysis. DAB staining.
Western blot - Anti-GAMT antibody (ab231656)
All lanes: Western blot - Anti-GAMT antibody (ab231656) at 2 µg/mL
Lane 1: Mouse liver tissue
Lane 2: Mouse testis tissue
Lane 3: Mouse brain tissue
SecondaryAll lanes: HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 26 kDa
Western blot - Anti-GAMT antibody (ab231656)
All lanes: Western blot - Anti-GAMT antibody (ab231656) at 2 µg/mL
All lanes: Recombinant mouse GAMT protein
SecondaryAll lanes: HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 26 kDa
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