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AB231656

Anti-GAMT antibody

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(1 Publication)

Rabbit Polyclonal GAMT antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Guanidinoacetate N-methyltransferase.

View Alternative Names

Guanidinoacetate N-methyltransferase, Gamt

10 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAMT antibody (AB231656)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAMT antibody (AB231656)

Paraffin-embedded mouse testis tissue stained for GAMT using ab231656 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAMT antibody (AB231656)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAMT antibody (AB231656)

Paraffin-embedded mouse liver tissue stained for GAMT using ab231656 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAMT antibody (AB231656)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAMT antibody (AB231656)

Paraffin-embedded mouse brain tissue stained for GAMT using ab231656 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAMT antibody (AB231656)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAMT antibody (AB231656)

Paraffin-embedded mouse liver tissue stained for GAMT using ab231656 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAMT antibody (AB231656)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAMT antibody (AB231656)

Paraffin-embedded mouse stomach tissue stained for GAMT using ab231656 at 10 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAMT antibody (AB231656)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAMT antibody (AB231656)

Paraffin-embedded mouse testis tissue stained for GAMT using ab231656 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAMT antibody (AB231656)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAMT antibody (AB231656)

Paraffin-embedded mouse uterus tissue stained for GAMT using ab231656 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAMT antibody (AB231656)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAMT antibody (AB231656)

Paraffin-embedded mouse uterus tissue stained for GAMT using ab231656 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Western blot - Anti-GAMT antibody (AB231656)
  • WB

Supplier Data

Western blot - Anti-GAMT antibody (AB231656)

All lanes:

Western blot - Anti-GAMT antibody (ab231656) at 2 µg/mL

Lane 1:

Mouse liver tissue

Lane 2:

Mouse testis tissue

Lane 3:

Mouse brain tissue

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

Predicted band size: 26 kDa

false

Western blot - Anti-GAMT antibody (AB231656)
  • WB

Supplier Data

Western blot - Anti-GAMT antibody (AB231656)

All lanes:

Western blot - Anti-GAMT antibody (ab231656) at 2 µg/mL

All lanes:

Recombinant mouse GAMT protein

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

Predicted band size: 26 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Mouse Guanidinoacetate N-methyltransferase.

O35969

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Immunohistochemistry (Frozen sections) - Anti-Dopamine Receptor D1 antibody [EPR24102-105] (AB279713)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab231656 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Guanidinoacetate N-methyltransferase commonly known as GAMT catalyzes the conversion of guanidinoacetate to creatine through methylation. The enzyme has a molecular mass of approximately 24 kDa. GAMT is expressed in various body tissues with high levels observed in the liver and kidney. The enzyme plays an important role in the synthesis of creatine an important molecule for energy storage and supply in muscle tissues.
Biological function summary

GAMT participates in the final step of creatine biosynthesis. This enzyme acts independently rather than forming part of a larger complex. In the cells creatine serves as an energy buffer predominantly involved in tissues with high and fluctuating energy demands such as muscles and the brain. GAMT's activity ensures a steady supply of creatine which supports cellular energy homeostasis.

Pathways

GAMT plays a significant role in the creatine biosynthesis pathway. This pathway is vital for maintaining the energy balance in cells especially those with a high-energy requirement. GAMT directly interacts with guanidinoacetate as a substrate with S-adenosylmethionine serving as the methyl group donor. Additionally GAMT is connected to the arginine and proline metabolism pathway alongside other enzymes such as arginine-glycine amidinotransferase (AGAT) which acts earlier in the creatine biosynthetic pathway.

Mutations or deficiencies in GAMT associate with disorders such as GAMT deficiency which leads to creatine deficiency syndromes. This condition typically results in neurological impairments including developmental delays and cognitive dysfunctions. The disorder also relates to disrupted creatine production in which AGAT and other enzymes within the creatine biosynthetic pathway may contribute to similar phenotypic outcomes. Research into these connections aids in understanding the broader implications of GAMT dysfunction.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Converts guanidinoacetate to creatine, using S-adenosylmethionine as the methyl donor. Important in nervous system development.
See full target information Guanidinoacetate N-methyltransferase
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

CNS neuroscience & therapeutics 30:e70098 PubMed39491498

2024

Fasudil Alleviates Postoperative Neurocognitive Disorders in Mice by Downregulating the Surface Expression of α5GABAAR in Hippocampus.

Applications

Unspecified application

Species

Unspecified reactive species

Jinpeng Dong,Zhun Wang,Lixuan Li,Mengxue Zhang,Sixuan Wang,Yuan Luo,Ying Dong,Xiaokun Wang,Yongan Wang,Kaiyuan Wang,Yiqing Yin
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Product promise

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