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Rabbit Recombinant Monoclonal GAMT antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPFlow CytWBICC/IF
Human
Tested
Not recommended
Not recommended
Tested
Not recommended
Rat
Predicted
Not recommended
Not recommended
Predicted
Not recommended

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Rat

Dilution info

-

Notes

-

Target data

Function

Converts guanidinoacetate to creatine, using S-adenosylmethionine as the methyl donor (PubMed:26003046, PubMed:24415674, PubMed:26319512). Important in nervous system development (PubMed:24415674).

Alternative names

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Rabbit Recombinant Monoclonal GAMT antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR6925

Purification technique

Affinity purification Protein A

Dissociation constant

7.4 x 10-11 M

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab248155 is the carrier-free version of ab126736.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

Biological function summary

GAMT participates in the final step of creatine biosynthesis. This enzyme acts independently rather than forming part of a larger complex. In the cells creatine serves as an energy buffer predominantly involved in tissues with high and fluctuating energy demands such as muscles and the brain. GAMT's activity ensures a steady supply of creatine which supports cellular energy homeostasis.

Activity summary

Guanidinoacetate N-methyltransferase commonly known as GAMT catalyzes the conversion of guanidinoacetate to creatine through methylation. The enzyme has a molecular mass of approximately 24 kDa. GAMT is expressed in various body tissues with high levels observed in the liver and kidney. The enzyme plays an important role in the synthesis of creatine an important molecule for energy storage and supply in muscle tissues.

Pathways

GAMT plays a significant role in the creatine biosynthesis pathway. This pathway is vital for maintaining the energy balance in cells especially those with a high-energy requirement. GAMT directly interacts with guanidinoacetate as a substrate with S-adenosylmethionine serving as the methyl group donor. Additionally GAMT is connected to the arginine and proline metabolism pathway alongside other enzymes such as arginine-glycine amidinotransferase (AGAT) which acts earlier in the creatine biosynthetic pathway.

Associated diseases and disorders

Mutations or deficiencies in GAMT associate with disorders such as GAMT deficiency which leads to creatine deficiency syndromes. This condition typically results in neurological impairments including developmental delays and cognitive dysfunctions. The disorder also relates to disrupted creatine production in which AGAT and other enzymes within the creatine biosynthetic pathway may contribute to similar phenotypic outcomes. Research into these connections aids in understanding the broader implications of GAMT dysfunction.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAMT antibody [EPR6925] - BSA and Azide free (ab248155), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAMT antibody [EPR6925] - BSA and Azide free (ab248155)

    This data was developed using ab126736, the same antibody clone in a different buffer formulation.ab126736 at 1/100 dilution, staining GAMT in Formalin-fixed, Paraffin-embedded Human liver tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-GAMT antibody [EPR6925] - BSA and Azide free (ab248155), expandable thumbnail

    Western blot - Anti-GAMT antibody [EPR6925] - BSA and Azide free (ab248155)

    This data was developed using ab126736, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-GAMT antibody [EPR6925] (AB126736) at 1/1000 dilution

    Lane 1: Human fetal liver Lysate at 10 µg

    Lane 2: HepG2 lysate at 10 µg

    Lane 3: SH-SY5Y lysate at 10 µg

    Secondary

    All lanes: Goat-anti-rabbit HRP at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 26 kDa

  • OI-RD Scanning - Anti-GAMT antibody [EPR6925] - BSA and Azide free (ab248155), expandable thumbnail

    OI-RD Scanning - Anti-GAMT antibody [EPR6925] - BSA and Azide free (ab248155)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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