AB96757

Anti-GANAB antibody

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Rabbit Polyclonal GANAB antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human GANAB aa 50-300.

View Alternative Names

G2AN, KIAA0088, GANAB, Neutral alpha-glucosidase AB, Alpha-glucosidase 2, Glucosidase II subunit alpha

7 Images

Immunocytochemistry/ Immunofluorescence - Anti-GANAB antibody (AB96757)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GANAB antibody (AB96757)

Mehanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells staibed forGANAB using ab96757 at1/500 dilution (green) in ICC/IF. Alpha tubulin filaments are detected with an anti-Alpha tubulin antibody (red).

Supplier Data

Western blot - Anti-GANAB antibody (AB96757)

All lanes:

Western blot - Anti-GANAB antibody (ab96757) at 1/1000 dilution

Lane 1:

Wild-type HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract at 30 µg

Lane 2:

GANAB knockout HeLa cell extract at 30 µg

Secondary

All lanes:

Anti-Rabbit IgG HRP

Predicted band size: 107 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GANAB antibody (AB96757)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GANAB antibody (AB96757)

Paraffin-embedded human breast carcinoma tissue stained for GANAB using ab96757 at 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Unknown

Western blot - Anti-GANAB antibody (AB96757)

7.5% SDS PAGE

All lanes:

Western blot - Anti-GANAB antibody (ab96757) at 1/1000 dilution

All lanes:

NIH-3T3 whole cell lysate at 30 µg

Predicted band size: 107 kDa

false

Unknown

Western blot - Anti-GANAB antibody (AB96757)

7.5% SDS PAGE

All lanes:

Western blot - Anti-GANAB antibody (ab96757) at 1/1000 dilution

Lane 1:

293T whole cell lysate at 30 µg

Lane 2:

A431 whole cell lysate at 30 µg

Lane 3:

H1299 whole cell lysate at 30 µg

Lane 4:

HeLa S3 whole cell lysate at 30 µg

Lane 5:

HepG2 whole cell lysate at 30 µg

Lane 6:

Molt-4 whole cell lysate at 30 µg

Lane 7:

Raji whole cell lysate at 30 µg

Predicted band size: 107 kDa

false

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GANAB antibody (AB96757)

Immunohistochemical analysis of GANAB in paraffin-embedded SW480 xenograft, using ab96757 at 1/500 dilution.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GANAB antibody (AB96757)

Immunofluorescence analysis of GANAB in methanol-fixed HeLa cells, using ab96757 at 1/200 dilution (1) and costained with Hoechst 33342 (2).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human GANAB aa 50-300. The exact immunogen used to generate this antibody is proprietary information.

Q14697

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500.00000 - 1/3000.00000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100.00000 - 1/500.00000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100.00000 - 1/500.00000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500.00000 - 1/3000.00000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GANAB also known as Glucosidase II alpha subunit or neutral alpha-glucosidase AB is an enzyme with an approximate mass of 110 kDa. It functions as part of the endoplasmic reticulum's quality control system modifying N-linked glycans on newly synthesized glycoproteins. GANAB cleaves glucose residues facilitating proper folding and maturation of glycoproteins. Expression of GANAB occurs mainly in the endoplasmic reticulum of various cell types indicating its broad relevance in cellular protein processing.

Biological function summary

GANAB performs a critical role in the maintenance of glycoprotein quality. It acts as part of the Glucosidase II complex working alongside a beta subunit to continue glycoprotein processing. Through the removal of glucose from N-glycans GANAB aids in the correct protein folding and prevents accumulation of misfolded proteins. This function is essential for the normal function of the endoplasmic reticulum-associated degradation (ERAD) pathway.

Pathways

Proper glycoprotein folding through GANAB activity integrates into the broader ERAD pathway and the unfolded protein response. GANAB's role complements the activities of other glycosylation-related proteins such as calnexin and calreticulin ensuring only correctly folded proteins advance through the secretory pathway. This integrated network maintains cellular homeostasis and prevents stress conditions in the endoplasmic reticulum.

GANAB's dysfunction associates with autosomal dominant polycystic liver disease and familial hyperinsulinism. These connections emphasize the importance of proper glycoprotein modification managed by GANAB. In these conditions alterations in GANAB or its interacting proteins can lead to aberrant cellular functions and consequent pathologies highlighting potential therapeutic targets in GANAB-associated pathways for disease management.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalytic subunit of glucosidase II that cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins (PubMed : 10929008). Required for PKD1/Polycystin-1 and PKD2/Polycystin-2 maturation and localization to the cell surface and cilia (PubMed : 27259053).

See full target information GANAB

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

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Associated Products

Select an associated product type

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