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AB179805

Anti-GANAB antibody [EPR12377(B)]

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(3 Publications)

Rabbit Recombinant Monoclonal GANAB antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

View Alternative Names

G2AN, KIAA0088, GANAB, Neutral alpha-glucosidase AB, Alpha-glucosidase 2, Glucosidase II subunit alpha

4 Images
Western blot - Anti-GANAB antibody [EPR12377(B)] (AB179805)
  • WB

Supplier Data

Western blot - Anti-GANAB antibody [EPR12377(B)] (AB179805)

All lanes:

Western blot - Anti-GANAB antibody [EPR12377(B)] (ab179805) at 1/1000 dilution

Lane 1:

Human placenta lysate at 10 µg

Lane 2:

A431 lysate at 10 µg

Lane 3:

HepG2 lysate at 10 µg

Lane 4:

HeLa lysate at 10 µg

Lane 5:

PC-12 lysate at 10 µg

Lane 6:

NIH3T3 lysate at 10 µg

Predicted band size: 107 kDa

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Flow Cytometry (Intracellular) - Anti-GANAB antibody [EPR12377(B)] (AB179805)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-GANAB antibody [EPR12377(B)] (AB179805)

Intracellular flow cytometric analysis of permeabilized HeLa cells labeling GANAB with ab179805 at 1/10 dilution (red), compared toa rabbit IgG negative control (green).

Immunoprecipitation - Anti-GANAB antibody [EPR12377(B)] (AB179805)
  • IP

Supplier Data

Immunoprecipitation - Anti-GANAB antibody [EPR12377(B)] (AB179805)

Western blot analysis on immunoprecipitation pellet from A431 cell lysate using ab179805.

All lanes:

Immunoprecipitation - Anti-GANAB antibody [EPR12377(B)] (ab179805)

Predicted band size: 107 kDa

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Western blot - Anti-GANAB antibody [EPR12377(B)] (AB179805)
  • WB

Lab

Western blot - Anti-GANAB antibody [EPR12377(B)] (AB179805)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : GANAB knockout HAP1 cell lysate (20 μg)
Lane 3 : HeLa cell lysate (20 μg)
Lane 4 : 1 cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab179805 observed at 105 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab179805 was shown to specifically react with GANAB when GANAB knockout samples were used. Wild-type and GANAB knockout samples were subjected to SDS-PAGE. ab179805 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-GANAB antibody [EPR12377(B)] (ab179805)

Predicted band size: 107 kDa

Observed band size: 105 kDa

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  • Carrier free

    Anti-GANAB antibody [EPR12377(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12377(B)

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IP, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GANAB also known as Glucosidase II alpha subunit or neutral alpha-glucosidase AB is an enzyme with an approximate mass of 110 kDa. It functions as part of the endoplasmic reticulum's quality control system modifying N-linked glycans on newly synthesized glycoproteins. GANAB cleaves glucose residues facilitating proper folding and maturation of glycoproteins. Expression of GANAB occurs mainly in the endoplasmic reticulum of various cell types indicating its broad relevance in cellular protein processing.
Biological function summary

GANAB performs a critical role in the maintenance of glycoprotein quality. It acts as part of the Glucosidase II complex working alongside a beta subunit to continue glycoprotein processing. Through the removal of glucose from N-glycans GANAB aids in the correct protein folding and prevents accumulation of misfolded proteins. This function is essential for the normal function of the endoplasmic reticulum-associated degradation (ERAD) pathway.

Pathways

Proper glycoprotein folding through GANAB activity integrates into the broader ERAD pathway and the unfolded protein response. GANAB's role complements the activities of other glycosylation-related proteins such as calnexin and calreticulin ensuring only correctly folded proteins advance through the secretory pathway. This integrated network maintains cellular homeostasis and prevents stress conditions in the endoplasmic reticulum.

GANAB's dysfunction associates with autosomal dominant polycystic liver disease and familial hyperinsulinism. These connections emphasize the importance of proper glycoprotein modification managed by GANAB. In these conditions alterations in GANAB or its interacting proteins can lead to aberrant cellular functions and consequent pathologies highlighting potential therapeutic targets in GANAB-associated pathways for disease management.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalytic subunit of glucosidase II that cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins (PubMed : 10929008). Required for PKD1/Polycystin-1 and PKD2/Polycystin-2 maturation and localization to the cell surface and cilia (PubMed : 27259053).
See full target information GANAB
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

American journal of physiology. Cell physiology 319:C1082-C1096 PubMed32938225

2020

Hydrogen sulfide accumulates LDL receptor precursor via downregulating PCSK9 in HepG2 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Huang,Ke Ning,Wen-Wen Li,Ge Lin,Cui-Lan Hou,Ming-Jie Wang,Yi-Chun Zhu

The Journal of clinical investigation 127:1772-1785 PubMed28375157

2017

Isolated polycystic liver disease genes define effectors of polycystin-1 function.

Applications

Unspecified application

Species

Unspecified reactive species

Whitney Besse,Ke Dong,Jungmin Choi,Sohan Punia,Sorin V Fedeles,Murim Choi,Anna-Rachel Gallagher,Emily B Huang,Ashima Gulati,James Knight,Shrikant Mane,Esa Tahvanainen,Pia Tahvanainen,Simone Sanna-Cherchi,Richard P Lifton,Terry Watnick,York P Pei,Vicente E Torres,Stefan Somlo

American journal of human genetics 98:1193-1207 PubMed27259053

2016

Mutations in GANAB, Encoding the Glucosidase IIα Subunit, Cause Autosomal-Dominant Polycystic Kidney and Liver Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Binu Porath,Vladimir G Gainullin,Emilie Cornec-Le Gall,Elizabeth K Dillinger,Christina M Heyer,Katharina Hopp,Marie E Edwards,Charles D Madsen,Sarah R Mauritz,Carly J Banks,Saurabh Baheti,Bharathi Reddy,José Ignacio Herrero,Jesús M Bañales,Marie C Hogan,Velibor Tasic,Terry J Watnick,Arlene B Chapman,Cécile Vigneau,Frédéric Lavainne,Marie-Pierre Audrézet,Claude Ferec,Yannick Le Meur,Vicente E Torres,Peter C Harris
View all publications
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