Rabbit Recombinant Monoclonal GANAB antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Images
![Immunoprecipitation - Anti-GANAB antibody [EPR12377(B)] - BSA and Azide free (AB250111), expandable thumbnail](https://content.abcam.com/products/images/ganab-antibody-epr12377b-bsa-and-azide-free-ab250111--immunoprecipitation-img41624.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-GANAB antibody [EPR12377(B)] - BSA and Azide free (AB250111)")
![Western blot - Anti-GANAB antibody [EPR12377(B)] - BSA and Azide free (AB250111), expandable thumbnail](https://content.abcam.com/products/images/ganab-antibody-epr12377b-bsa-and-azide-free-ab250111--western-blot-img138365.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GANAB antibody [EPR12377(B)] - BSA and Azide free (AB250111)")
![Flow Cytometry (Intracellular) - Anti-GANAB antibody [EPR12377(B)] - BSA and Azide free (AB250111), expandable thumbnail](https://content.abcam.com/products/images/ganab-antibody-epr12377b-bsa-and-azide-free-ab250111--flow-cytometry-intracellular-img26866.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-GANAB antibody [EPR12377(B)] - BSA and Azide free (AB250111)")
![Western blot - Anti-GANAB antibody [EPR12377(B)] - BSA and Azide free (AB250111), expandable thumbnail](https://content.abcam.com/products/images/ganab-antibody-epr12377b-bsa-and-azide-free-ab250111--western-blot-img99868.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GANAB antibody [EPR12377(B)] - BSA and Azide free (AB250111)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Not recommended | Tested |
| Mouse | Expected | Tested | Not recommended | Not recommended | Expected |
| Rat | Expected | Tested | Not recommended | Not recommended | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Catalytic subunit of glucosidase II that cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins (PubMed:10929008). Required for PKD1/Polycystin-1 and PKD2/Polycystin-2 maturation and localization to the cell surface and cilia (PubMed:27259053).
Alternative names
G2AN, KIAA0088, GANAB, Neutral alpha-glucosidase AB, Alpha-glucosidase 2, Glucosidase II subunit alpha
Recommended products
Rabbit Recombinant Monoclonal GANAB antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR12377(B)
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab250111 is the carrier-free version of Anti-GANAB antibody [EPR12377(B)] ab179805.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GANAB also known as Glucosidase II alpha subunit or neutral alpha-glucosidase AB is an enzyme with an approximate mass of 110 kDa. It functions as part of the endoplasmic reticulum's quality control system modifying N-linked glycans on newly synthesized glycoproteins. GANAB cleaves glucose residues facilitating proper folding and maturation of glycoproteins. Expression of GANAB occurs mainly in the endoplasmic reticulum of various cell types indicating its broad relevance in cellular protein processing.
- Biological function summary
GANAB performs a critical role in the maintenance of glycoprotein quality. It acts as part of the Glucosidase II complex working alongside a beta subunit to continue glycoprotein processing. Through the removal of glucose from N-glycans GANAB aids in the correct protein folding and prevents accumulation of misfolded proteins. This function is essential for the normal function of the endoplasmic reticulum-associated degradation (ERAD) pathway.
- Pathways
Proper glycoprotein folding through GANAB activity integrates into the broader ERAD pathway and the unfolded protein response. GANAB's role complements the activities of other glycosylation-related proteins such as calnexin and calreticulin ensuring only correctly folded proteins advance through the secretory pathway. This integrated network maintains cellular homeostasis and prevents stress conditions in the endoplasmic reticulum.
- Associated diseases and disorders
GANAB's dysfunction associates with autosomal dominant polycystic liver disease and familial hyperinsulinism. These connections emphasize the importance of proper glycoprotein modification managed by GANAB. In these conditions alterations in GANAB or its interacting proteins can lead to aberrant cellular functions and consequent pathologies highlighting potential therapeutic targets in GANAB-associated pathways for disease management.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunoprecipitation - Anti-GANAB antibody [EPR12377(B)] - BSA and Azide free (ab250111), expandable thumbnail](https://content.abcam.com/products/images/ganab-antibody-epr12377b-bsa-and-azide-free-ab250111--immunoprecipitation-img41624.jpg "Immunoprecipitation - Anti-GANAB antibody [EPR12377(B)] - BSA and Azide free (ab250111)")
Immunoprecipitation - Anti-GANAB antibody [EPR12377(B)] - BSA and Azide free (ab250111)
This data was developed using Anti-GANAB antibody [EPR12377(B)] ab179805, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from A431 cell lysate using Anti-GANAB antibody [EPR12377(B)] ab179805.
All lanes: Immunoprecipitation - Anti-GANAB antibody [EPR12377(B)] (Anti-GANAB antibody [EPR12377(B)] ab179805)
Predicted band size: 107 kDa
![Western blot - Anti-GANAB antibody [EPR12377(B)] - BSA and Azide free (ab250111), expandable thumbnail](https://content.abcam.com/products/images/ganab-antibody-epr12377b-bsa-and-azide-free-ab250111--western-blot-img138365.jpg "Western blot - Anti-GANAB antibody [EPR12377(B)] - BSA and Azide free (ab250111)")
Western blot - Anti-GANAB antibody [EPR12377(B)] - BSA and Azide free (ab250111)
This data was developed using Anti-GANAB antibody [EPR12377(B)] ab179805, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 cell lysate (20 μg)
Lane 2: GANAB knockout HAP1 cell lysate (20 μg)
Lane 3: HeLa cell lysate (20 μg)
Lane 4: cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - Anti-GANAB antibody [EPR12377(B)] ab179805 observed at 105 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
Anti-GANAB antibody [EPR12377(B)] ab179805 was shown to specifically react with GANAB when GANAB knockout samples were used. Wild-type and GANAB knockout samples were subjected to SDS-PAGE. Anti-GANAB antibody [EPR12377(B)] ab179805 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-GANAB antibody [EPR12377(B)] (Anti-GANAB antibody [EPR12377(B)] ab179805)
Predicted band size: 107 kDa
Observed band size: 105 kDa
![Flow Cytometry (Intracellular) - Anti-GANAB antibody [EPR12377(B)] - BSA and Azide free (ab250111), expandable thumbnail](https://content.abcam.com/products/images/ganab-antibody-epr12377b-bsa-and-azide-free-ab250111--flow-cytometry-intracellular-img26866.jpg "Flow Cytometry (Intracellular) - Anti-GANAB antibody [EPR12377(B)] - BSA and Azide free (ab250111)")
Flow Cytometry (Intracellular) - Anti-GANAB antibody [EPR12377(B)] - BSA and Azide free (ab250111)
This data was developed using Anti-GANAB antibody [EPR12377(B)] ab179805, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of permeabilized HeLa cells labeling GANAB with Anti-GANAB antibody [EPR12377(B)] ab179805 at 1/10 dilution (red), compared to a rabbit IgG negative control (green).
![Western blot - Anti-GANAB antibody [EPR12377(B)] - BSA and Azide free (ab250111), expandable thumbnail](https://content.abcam.com/products/images/ganab-antibody-epr12377b-bsa-and-azide-free-ab250111--western-blot-img99868.jpg "Western blot - Anti-GANAB antibody [EPR12377(B)] - BSA and Azide free (ab250111)")
Western blot - Anti-GANAB antibody [EPR12377(B)] - BSA and Azide free (ab250111)
This data was developed using Anti-GANAB antibody [EPR12377(B)] ab179805, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-GANAB antibody [EPR12377(B)] (Anti-GANAB antibody [EPR12377(B)] ab179805) at 1/1000 dilution
Lane 1: Human placenta lysate at 10 µg
Lane 2: A431 lysate at 10 µg
Lane 3: HepG2 lysate at 10 µg
Lane 4: HeLa lysate at 10 µg
Lane 5: PC-12 lysate at 10 µg
Lane 6: NIH3T3 lysate at 10 µg
Predicted band size: 107 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com