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AB11779

Anti-Ganglioside GD3 antibody [R24]

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(27 Publications)

Mouse Monoclonal Ganglioside GD3 antibody. Suitable for IHC-Fr, IHC-P, ICC/IF and reacts with Human samples. Cited in 27 publications. Immunogen corresponding to Cell preparation containing Ganglioside GD3 protein.

View Alternative Names

GD3

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Ganglioside GD3 antibody [R24] (AB11779)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Ganglioside GD3 antibody [R24] (AB11779)

ICC/IF image of ab11779 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11779, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Immunohistochemistry (Frozen sections) - Anti-Ganglioside GD3 antibody [R24] (AB11779)
  • IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Ganglioside GD3 antibody [R24] (AB11779)

Frozen section of human melanoma tissue stained for Ganglioside GD3 with ab11779 at 1/40 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ganglioside GD3 antibody [R24] (AB11779)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ganglioside GD3 antibody [R24] (AB11779)

IHC image of ab11779 staining in human melanoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11779, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

R24

Isotype

IgG3

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

IHC-Fr, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/40", "IHCFr-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Affinity purified from tissue culture supernatant.
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ganglioside GD3 also known as GD3 ganglioside is a sialic acid-containing glycosphingolipid. It has a molecular weight of approximately 1231 g/mol. This lipid is mainly expressed in the outer layer of cell membranes particularly in neural tissues and melanoma cells. The presence of GD3 is a signature of the cell's developmental stage and is often found in early embryonic stages and certain types of tumor cells.
Biological function summary

Ganglioside GD3 participates in cell signaling proliferation and apoptosis. It often operates in complex with proteins within lipid rafts specialized microdomains of the plasma membrane. GD3 assists in modifying cell-cell interactions and communication playing a role in cellular response to external stimuli. Its presence significantly influences the dynamics of cellular membranes by affecting the fluidity and formation of microdomains.

Pathways

Cell signaling and apoptotic pathways feature important roles carried by ganglioside GD3. GD3 interacts with proteins such as protein kinase C and Src family kinases modulating signaling cascades that govern cell survival and apoptosis. Through these interactions GD3 influences cellular stress responses and can switch cells between survival and death pathways.

Ganglioside GD3 is notably linked to melanoma and neurodegenerative diseases. High levels of GD3 expression often correlate with aggressive forms of melanoma where it impacts cell adhesion and migration. In neurodegenerative disorders like Alzheimer's disease abnormal metabolism of GD3 may disrupt neuron function and contribute to neuronal loss. The target has connections to Bcl-2 family proteins which mediate pathways related to programmed cell death emphasizing its role in both oncogenesis and neurodegeneration.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Ganglioside GD3
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Publications (27)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell international 25:246 PubMed40604768

2025

The GD3 ganglioside promotes cell growth, plasticity and chemotherapy resistance of human glioblastoma cancer stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Victoria Hein,Nathalie Baeza-Kallee,Raphaël Bergès,Nora Essakhi,Aurélie Soubéran,Carole Colin,Philippe Morando,Romain Appay,Thomas Graillon,Aurélie Tchoghandjian,Dominique Figarella-Branger,Emeline Tabouret

The European journal of neuroscience 61:e70061 PubMed40091288

2025

Gene Therapy Targeting GD3 Synthase Protects Against MPTP-Induced Parkinsonism and Executive Dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Panchanan Maiti,Yi Xue,Tonia S Rex,Michael P McDonald

Nature aging 5:219-236 PubMed39730825

2024

A ganglioside-based immune checkpoint enables senescent cells to evade immunosurveillance during aging.

Applications

Unspecified application

Species

Unspecified reactive species

Charlène Iltis,Iryna Moskalevska,Antoine Debiesse,Laetitia Seguin,Christina Fissoun,Ludovic Cervera,Lyvia Moudombi,Maude Ardin,Anthony Ferrari,Coline Eliott,Didier Pisani,Alexandre Ottaviani,Manon Bourinet,Carmelo Luci,Philippe Gual,Gabriela Makulyte,David Bernard,Manon Durandy,Lou C Duret,Tynhinane Hamidouche,Sarah Kunz,Olivier Croce,Clément Delannoy,Yann Guérardel,Fabrice Allain,Paul Hofman,Delphine Benarroch-Popivker,Laurence Bianchini,Berengère Dadone-Montaudie,Estelle Cosson,Julien Guglielmi,Thierry Pourcher,Véronique M Braud,Marina Shkreli,Yves-Marie Pers,Christian Jorgensen,Jean-Marc Brondello,Chloé C Féral,Marie-Cécile Michallet,Eric Gilson,Julien Cherfils-Vicini

International journal of molecular sciences 25: PubMed38892363

2024

Autophagy Promotes Enrichment of Raft Components within Extracellular Vesicles Secreted by Human 2FTGH Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Valeria Manganelli,Luciana Dini,Stefano Tacconi,Simone Dinarelli,Antonella Capozzi,Gloria Riitano,Serena Recalchi,Tuba Rana Caglar,Federica Fratini,Roberta Misasi,Maurizio Sorice,Tina Garofalo

Frontiers in oncology 13:1134763 PubMed37124505

2023

GD2 and GD3 gangliosides as diagnostic biomarkers for all stages and subtypes of epithelial ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Alba Galan,Arturo Papaluca,Ali Nejatie,Emad Matanes,Fouad Brahimi,Wenyong Tong,Ibrahim Yaseen Hachim,Amber Yasmeen,Euridice Carmona,Kathleen Oros Klein,Sonja Billes,Ahmed E Dawod,Prasad Gawande,Anna Milik Jeter,Anne-Marie Mes-Masson,Celia M T Greenwood,Walter H Gotlieb,H Uri Saragovi

Journal of neurochemistry 158:737-752 PubMed34133773

2021

Ganglioside GD3 is up-regulated in microglia and regulates phagocytosis following global cerebral ischemia.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Wang,Quanguang Zhang,Yujiao Lu,Yan Dong,Krishnan M Dhandapani,Darrell W Brann,Robert K Yu

Journal of neurochemistry 158:694-709 PubMed34081777

2021

GD3 synthase deletion alters retinal structure and impairs visual function in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Carla Andreia Abreu,Leandro Coelho Teixeira-Pinheiro,Rafael Lani-Louzada,Almir Jordão da Silva-Junior,Juliana F Vasques,Fernanda Gubert,Gabriel Nascimento-Dos-Santos,Ronaldo Mohana-Borges,Eduardo de Souza Matos,Pedro Moreno Pimentel-Coelho,Marcelo Felippe Santiago,Rosalia Mendez-Otero

Journal of clinical medicine 9: PubMed32545211

2020

Sialylation of Human Natural Killer (NK) Cells is Regulated by IL-2.

Applications

Unspecified application

Species

Unspecified reactive species

Philip Rosenstock,Kaya Bork,Chiara Massa,Philipp Selke,Barbara Seliger,Rüdiger Horstkorte

Molecular oncology 14:1760-1778 PubMed32358995

2020

Upregulation of cell surface GD3 ganglioside phenotype is associated with human melanoma brain metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Romela Irene Ramos,Matias A Bustos,Jinfeng Wu,Peter Jones,Shu Ching Chang,Eiji Kiyohara,Kevin Tran,Xiaoqing Zhang,Stacey L Stern,Sivan Izraely,Orit Sagi-Assif,Isaac P Witz,Michael A Davies,Gordon B Mills,Daniel F Kelly,Reiko F Irie,Dave S B Hoon

Nature microbiology 5:1069-1078 PubMed32451473

2020

Gangliosides are essential endosomal receptors for quasi-enveloped and naked hepatitis A virus.

Applications

Unspecified application

Species

Unspecified reactive species

Anshuman Das,Rodell Barrientos,Tomoyuki Shiota,Victoria Madigan,Ichiro Misumi,Kevin L McKnight,Lu Sun,Zhucui Li,Rita M Meganck,You Li,Ewelina Kaluzna,Aravind Asokan,Jason K Whitmire,Maryna Kapustina,Qibin Zhang,Stanley M Lemon
View all publications
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Product promise

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