AB188315

Anti-Gankyrin antibody [EPR14456(2)]

Name: Anti-Gankyrin antibody [EPR14456(2)] (ab188315) | Abcam
Brand: Abcam Limited
SKU: AB188315
Price: 340 USD
Availability: InStock
Rating: 5 (4 reviews)

RabMAb
Recombinant
KO Validated
What is this?

(4 Reviews)

(2 Publications)

Rabbit Recombinant Monoclonal Gankyrin antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Rat, Human, Mouse samples. Cited in 2 publications.

View Alternative Names

26S proteasome non-ATPase regulatory subunit 10, 26S proteasome regulatory subunit p28, Gankyrin, p28(GANK), PSMD10

5 Images

Flow Cytometry (Intracellular) - Anti-Gankyrin antibody [EPR14456(2)] (AB188315)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Gankyrin antibody [EPR14456(2)] (AB188315)

Intracellular flow cytometric analysis of HeLa cells fixed in 2% paraformaldehyde labeling Gankyrin with ab188315 at 1/110 dilution and Goat anti-rabbit IgG (FITC) at 1/150 dilution. Rabbit monoclonal IgG was used as an isotype control.

Lab

Western blot - Anti-Gankyrin antibody [EPR14456(2)] (AB188315)

Lanes 1-4 : Merged signal (red and green). Green - ab188315 observed at 24 kDa. Red - loading control ab8245 observed at 36 kDa.

ab188315 Anti-Gankyrin antibody [EPR14456(2)] was shown to specifically react with Gankyrin in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265680 (knockout cell lysate ab258146) was used. Wild-type and Gankyrin knockout samples were subjected to SDS-PAGE. ab188315 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Gankyrin antibody [EPR14456(2)] (ab188315) at 1/1000 dilution

Lane 1:

Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

PSMD10 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

Western blot - Human PSMD10 (Gankyrin) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-psmd10-gankyrin-knockout-hela-cell-line-ab265680'>ab265680</a>)

Lane 3:

K562 (Human chronic myelogenous leukemia lymphoblast cell line) whole cell lysate at 20 µg

Lane 4:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 24 kDa

Observed band size: 24 kDa

false

Supplier Data

Western blot - Anti-Gankyrin antibody [EPR14456(2)] (AB188315)

All lanes:

Western blot - Anti-Gankyrin antibody [EPR14456(2)] (ab188315) at 1/1000 dilution

Lane 1:

C6 cell lysate at 10 µg

Lane 2:

Raw 264.7 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 24 kDa

false

Supplier Data

Western blot - Anti-Gankyrin antibody [EPR14456(2)] (AB188315)

All lanes:

Western blot - Anti-Gankyrin antibody [EPR14456(2)] (ab188315) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 20 µg

Lane 2:

293 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 24 kDa

false

Supplier Data

Western blot - Anti-Gankyrin antibody [EPR14456(2)] (AB188315)

All lanes:

Western blot - Anti-Gankyrin antibody [EPR14456(2)] (ab188315) at 1/10000 dilution

Lane 1:

K562 cell lysate at 20 µg

Lane 2:

Hela cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 24 kDa

false

Carrier free

Anti-Gankyrin antibody [EPR14456(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14456(2)

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/110", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Gankyrin or PSMD10 is an oncogene with a molecular mass of around 25 kDa. It is a small heat-stable protein predominantly expressed in the cytoplasm of various tissues. The protein contains multiple ankyrin repeats that facilitate protein-protein interactions. It is overexpressed in many types of cancer tissues including liver breast and lung cancer. Gankyrin interacts with various cell cycle regulators and serves as an adaptor molecule enhancing its significance in cellular processes.

Biological function summary

Gankyrin enhances the degradation of the retinoblastoma protein (pRb) as part of the 26S proteasome complex. This function disrupts normal cell cycle control promoting cell proliferation. Gankyrin binds directly to the cyclin-dependent kinase 4 (CDK4) and MDM2 which regulate pRb and p53 tumor suppressor pathways. Overexpression of Gankyrin contributes to cellular transformation and oncogenesis by regulating cell cycle progression and apoptosis.

Pathways

Gankyrin participates actively in p53 and ubiquitin-proteasome pathways. The protein facilitates ubiquitination and degradation of key regulators including p53 and pRb altering the stability of these proteins. Gankyrin interacts with proteins like MDM2 and HDAC1 influencing their roles in tumor development through these pathways. Its presence in these pathways contributes to unchecked cell growth by evading normal tumor suppressor mechanisms.

Gankyrin has strong associations with cancer particularly in hepatocellular carcinoma and colorectal cancer. In these cancers it influences tumor progression and metastasis by altering the functions of proteins within the p53 pathway such as MDM2 and pRb. In hepatocellular carcinoma increased Gankyrin activity correlates with poor prognosis and aggressive tumor behavior while in colorectal cancer its interaction with these proteins may contribute to chemotherapy resistance.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD10 : PSMC4 : PSMC5 : PAAF1 module which probably assembles with a PSMD5 : PSMC2 : PSMC1 : PSMD2 module. Independently of the proteasome, regulates EGF-induced AKT activation through inhibition of the RHOA/ROCK/PTEN pathway, leading to prolonged AKT activation. Plays an important role in RAS-induced tumorigenesis.. Acts as an proto-oncoprotein by being involved in negative regulation of tumor suppressors RB1 and p53/TP53. Overexpression is leading to phosphorylation of RB1 and proteasomal degradation of RB1. Regulates CDK4-mediated phosphorylation of RB1 by competing with CDKN2A for binding with CDK4. Facilitates binding of MDM2 to p53/TP53 and the mono- and polyubiquitination of p53/TP53 by MDM2 suggesting a function in targeting the TP53 : MDM2 complex to the 26S proteasome. Involved in p53-independent apoptosis. Involved in regulation of NF-kappa-B by retaining it in the cytoplasm. Binds to the NF-kappa-B component RELA and accelerates its XPO1/CRM1-mediated nuclear export.

See full target information 26S proteasome non-ATPase regulatory subunit 10

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature genetics 56:1654-1664 PubMed39048795

2024

Cohesin prevents cross-domain gene coactivation.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Dong,Shu Zhang,Valentina Gandin,Liangqi Xie,Lihua Wang,Andrew L Lemire,Wenhong Li,Hideo Otsuna,Takashi Kawase,Arthur D Lander,Howard Y Chang,Zhe J Liu

eLife 10: PubMed34254583

2021

Genetically incorporated crosslinkers reveal NleE attenuates host autophagy dependent on PSMD10.

Applications

Unspecified application

Species

Unspecified reactive species

Jingxiang Li,Shupan Guo,Fangni Chai,Qi Sun,Pan Li,Li Gao,Lunzhi Dai,Xiaoxiao Ouyang,Zhihui Zhou,Li Zhou,Wei Cheng,Shiqian Qi,Kefeng Lu,Haiyan Ren

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Gankyrin antibody [EPR14456(2)]

Flow Cytometry (Intracellular) - Anti-Gankyrin antibody [EPR14456(2)] (AB188315)

Western blot - Anti-Gankyrin antibody [EPR14456(2)] (AB188315)

Western blot - Anti-Gankyrin antibody [EPR14456(2)] (AB188315)

Western blot - Anti-Gankyrin antibody [EPR14456(2)] (AB188315)

Western blot - Anti-Gankyrin antibody [EPR14456(2)] (AB188315)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise