AB113295

Anti-GANP antibody

Name: Anti-GANP antibody (ab113295) | Abcam
Brand: Abcam Limited
SKU: AB113295

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(3 Publications)

Rabbit Polyclonal GANP antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human MCM3AP aa 1-50.

View Alternative Names

GANP, KIAA0572, MAP80, MCM3AP, Germinal-center associated nuclear protein, 80 kDa MCM3-associated protein, MCM3 acetylating protein, MCM3 acetyltransferase

1 Images

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Western blot - Anti-GANP antibody (AB113295)

All lanes:

Western blot - Anti-GANP antibody (ab113295) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 218 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human MCM3AP aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

O60318

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GANP also known as Germinal Center Associated Nuclear Protein and encoded by the MCM3AP gene is a protein with a molecular mass of approximately 210 kDa. GANP shows expression in various tissues most notably in germinal center B cells within lymphoid organs. It also appears in other cell types but at lower levels. The structure of GANP reflects its multifunctional role in cellular processes particularly in the context of immune function and cell cycle regulation.

Biological function summary

The GANP protein plays an essential role in DNA replication and repair mechanisms. It contributes to immune responses by facilitating the maturation of antibody-producing B cells. GANP functions as a part of a larger protein complex often associating with other nucleic acid-binding proteins. Its activity supports somatic hypermutation and class switch recombination processes critical for the diversification of the antibody repertoire.

Pathways

GANP integrates into the DNA replication pathway and the antigen receptor-mediated signaling pathway. In the DNA replication pathway GANP works alongside proteins like MCM3 and other components of the replication machinery to ensure accurate DNA synthesis. In the context of immune signaling GANP interacts with various signaling molecules enhancing adaptive immune responses through its regulatory functions.

GANP involvement connects primarily to immunological diseases and certain cancers. Aberrations in GANP function or expression can associate with autoimmune disorders due to its impact on immune cell regulation. In cancers GANP expression has been observed in malignancies where it may contribute to unchecked cellular proliferation. The MCM3 protein a close functional associate of GANP highlights its role in pathways that when disrupted potentially lead to tumorigenesis and other immune-related pathologies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Isoform GANP. As a component of the TREX-2 complex, involved in the export of mRNAs to the cytoplasm through the nuclear pores (PubMed : 20005110, PubMed : 20384790, PubMed : 22307388, PubMed : 23591820). Through the acetylation of histones, affects the assembly of nucleosomes at immunoglobulin variable region genes and promotes the recruitment and positioning of transcription complex to favor DNA cytosine deaminase AICDA/AID targeting, hence promoting somatic hypermutations (PubMed : 23652018).. Isoform MCM3AP. Binds to and acetylates the replication protein MCM3. Plays a role in the initiation of DNA replication and participates in controls that ensure that DNA replication initiates only once per cell cycle (PubMed : 11258703, PubMed : 12226073). Through the acetylation of histones, affects the assembly of nucleosomes at immunoglobulin variable region genes and promotes the recruitment and positioning of transcription complex to favor DNA cytosine deaminase AICDA/AID targeting, hence promoting somatic hypermutations (PubMed : 23652018).

See full target information MCM3AP

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 12:3937 PubMed34168151

2021

The human nucleoporin Tpr protects cells from RNA-mediated replication stress.

Applications

Unspecified application

Species

Unspecified reactive species

Martin Kosar,Michele Giannattasio,Daniele Piccini,Apolinar Maya-Mendoza,Francisco García-Benítez,Jirina Bartkova,Sonia I Barroso,Hélène Gaillard,Emanuele Martini,Umberto Restuccia,Miguel Angel Ramirez-Otero,Massimiliano Garre,Eleonora Verga,Miguel Andújar-Sánchez,Scott Maynard,Zdenek Hodny,Vincenzo Costanzo,Amit Kumar,Angela Bachi,Andrés Aguilera,Jiri Bartek,Marco Foiani

Human molecular genetics 29:1426-1439 PubMed32202298

2020

Distinct effects on mRNA export factor GANP underlie neurological disease phenotypes and alter gene expression depending on intron content.

Applications

Unspecified application

Species

Unspecified reactive species

Rosa Woldegebriel,Jouni Kvist,Noora Andersson,Katrin Õunap,Karit Reinson,Monica H Wojcik,Emilia K Bijlsma,Mariëtte J V Hoffer,Monique M Ryan,Zornitza Stark,Maie Walsh,Inge Cuppen,Marie-Jose H van den Boogaard,Diana Bharucha-Goebel,Sandra Donkervoort,Sara Winchester,Roberto Zori,Carsten G Bönnemann,Reza Maroofian,Emer O'Connor,Henry Houlden,Fang Zhao,Olli Carpén,Matthew White,Jemeen Sreedharan,Murray Stewart,Emil Ylikallio,Henna Tyynismaa

Journal of cell science 126:2656-67 PubMed23591820

2013

The human TREX-2 complex is stably associated with the nuclear pore basket.

Applications

Unspecified application

Species

Human

David Umlauf,Jacques Bonnet,François Waharte,Marjorie Fournier,Matthieu Stierle,Benoit Fischer,Laurent Brino,Didier Devys,László Tora

View all publications

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Product promise

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Anti-GANP antibody

Western blot - Anti-GANP antibody (AB113295)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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