AB224434

Anti-GANP antibody

Name: Anti-GANP antibody (ab224434) | Abcam
Brand: Abcam Limited
SKU: AB224434

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(1 Publication)

Rabbit Polyclonal GANP antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MCM3AP aa 300-450.

View Alternative Names

GANP, KIAA0572, MAP80, MCM3AP, Germinal-center associated nuclear protein, 80 kDa MCM3-associated protein, MCM3 acetylating protein, MCM3 acetyltransferase

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-GANP antibody (AB224434)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GANP antibody (AB224434)

Immunofluorescent analysis of U-251 MG (human brain glioma cell line) cells labeling GANP (green) in nuclear membrane and cytosol with ab224434 at 2 μg/ml.

Supplier Data

Western blot - Anti-GANP antibody (AB224434)

All lanes:

Western blot - Anti-GANP antibody (ab224434) at 0.4 µg/mL

All lanes:

K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate

Predicted band size: 218 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human MCM3AP aa 300-450. The exact immunogen used to generate this antibody is proprietary information.

O60318

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GANP also known as Germinal Center Associated Nuclear Protein and encoded by the MCM3AP gene is a protein with a molecular mass of approximately 210 kDa. GANP shows expression in various tissues most notably in germinal center B cells within lymphoid organs. It also appears in other cell types but at lower levels. The structure of GANP reflects its multifunctional role in cellular processes particularly in the context of immune function and cell cycle regulation.

Biological function summary

The GANP protein plays an essential role in DNA replication and repair mechanisms. It contributes to immune responses by facilitating the maturation of antibody-producing B cells. GANP functions as a part of a larger protein complex often associating with other nucleic acid-binding proteins. Its activity supports somatic hypermutation and class switch recombination processes critical for the diversification of the antibody repertoire.

Pathways

GANP integrates into the DNA replication pathway and the antigen receptor-mediated signaling pathway. In the DNA replication pathway GANP works alongside proteins like MCM3 and other components of the replication machinery to ensure accurate DNA synthesis. In the context of immune signaling GANP interacts with various signaling molecules enhancing adaptive immune responses through its regulatory functions.

GANP involvement connects primarily to immunological diseases and certain cancers. Aberrations in GANP function or expression can associate with autoimmune disorders due to its impact on immune cell regulation. In cancers GANP expression has been observed in malignancies where it may contribute to unchecked cellular proliferation. The MCM3 protein a close functional associate of GANP highlights its role in pathways that when disrupted potentially lead to tumorigenesis and other immune-related pathologies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Isoform GANP. As a component of the TREX-2 complex, involved in the export of mRNAs to the cytoplasm through the nuclear pores (PubMed : 20005110, PubMed : 20384790, PubMed : 22307388, PubMed : 23591820). Through the acetylation of histones, affects the assembly of nucleosomes at immunoglobulin variable region genes and promotes the recruitment and positioning of transcription complex to favor DNA cytosine deaminase AICDA/AID targeting, hence promoting somatic hypermutations (PubMed : 23652018).. Isoform MCM3AP. Binds to and acetylates the replication protein MCM3. Plays a role in the initiation of DNA replication and participates in controls that ensure that DNA replication initiates only once per cell cycle (PubMed : 11258703, PubMed : 12226073). Through the acetylation of histones, affects the assembly of nucleosomes at immunoglobulin variable region genes and promotes the recruitment and positioning of transcription complex to favor DNA cytosine deaminase AICDA/AID targeting, hence promoting somatic hypermutations (PubMed : 23652018).

See full target information MCM3AP

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:8324 PubMed38594513

2024

A study on the significance of serine hydroxymethyl transferase expression and its role in bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Si-Wei Su,Xian Chen,Guang Wang,Pei Li,Tong-Xin Yang,Ke-Wei Fang,Jing Wu,Jiong-Ming Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GANP antibody

Immunocytochemistry/ Immunofluorescence - Anti-GANP antibody (AB224434)

Western blot - Anti-GANP antibody (AB224434)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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