Rabbit Polyclonal GANP antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MCM3AP aa 300-450.
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Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human MCM3AP aa 300-450. The exact immunogen used to generate this antibody is proprietary information. Database link O60318
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Isoform GANP. As a component of the TREX-2 complex, involved in the export of mRNAs to the cytoplasm through the nuclear pores (PubMed:20005110, PubMed:20384790, PubMed:22307388, PubMed:23591820). Through the acetylation of histones, affects the assembly of nucleosomes at immunoglobulin variable region genes and promotes the recruitment and positioning of transcription complex to favor DNA cytosine deaminase AICDA/AID targeting, hence promoting somatic hypermutations (PubMed:23652018). Isoform MCM3AP. Binds to and acetylates the replication protein MCM3. Plays a role in the initiation of DNA replication and participates in controls that ensure that DNA replication initiates only once per cell cycle (PubMed:11258703, PubMed:12226073). Through the acetylation of histones, affects the assembly of nucleosomes at immunoglobulin variable region genes and promotes the recruitment and positioning of transcription complex to favor DNA cytosine deaminase AICDA/AID targeting, hence promoting somatic hypermutations (PubMed:23652018).
Alternative names
GANP, KIAA0572, MAP80, MCM3AP, Germinal-center associated nuclear protein, 80 kDa MCM3-associated protein, MCM3 acetylating protein, MCM3 acetyltransferase
Recommended products
Rabbit Polyclonal GANP antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MCM3AP aa 300-450.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human MCM3AP aa 300-450. The exact immunogen used to generate this antibody is proprietary information. Database link O60318
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GANP also known as Germinal Center Associated Nuclear Protein and encoded by the MCM3AP gene is a protein with a molecular mass of approximately 210 kDa. GANP shows expression in various tissues most notably in germinal center B cells within lymphoid organs. It also appears in other cell types but at lower levels. The structure of GANP reflects its multifunctional role in cellular processes particularly in the context of immune function and cell cycle regulation.
- Biological function summary
The GANP protein plays an essential role in DNA replication and repair mechanisms. It contributes to immune responses by facilitating the maturation of antibody-producing B cells. GANP functions as a part of a larger protein complex often associating with other nucleic acid-binding proteins. Its activity supports somatic hypermutation and class switch recombination processes critical for the diversification of the antibody repertoire.
- Pathways
GANP integrates into the DNA replication pathway and the antigen receptor-mediated signaling pathway. In the DNA replication pathway GANP works alongside proteins like MCM3 and other components of the replication machinery to ensure accurate DNA synthesis. In the context of immune signaling GANP interacts with various signaling molecules enhancing adaptive immune responses through its regulatory functions.
- Associated diseases and disorders
GANP involvement connects primarily to immunological diseases and certain cancers. Aberrations in GANP function or expression can associate with autoimmune disorders due to its impact on immune cell regulation. In cancers GANP expression has been observed in malignancies where it may contribute to unchecked cellular proliferation. The MCM3 protein a close functional associate of GANP highlights its role in pathways that when disrupted potentially lead to tumorigenesis and other immune-related pathologies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-GANP antibody (ab224434)
All lanes: Western blot - Anti-GANP antibody (ab224434) at 0.4 µg/mL
All lanes: K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
Predicted band size: 218 kDa
Immunocytochemistry/ Immunofluorescence - Anti-GANP antibody (ab224434)
Immunofluorescent analysis of U-251 MG (human brain glioma cell line) cells labeling GANP (green) in nuclear membrane and cytosol with ab224434 at 2 μg/ml.
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