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Rabbit Polyclonal GANP antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MCM3AP aa 300-450.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human MCM3AP aa 300-450. The exact immunogen used to generate this antibody is proprietary information. Database link O60318
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

0.04000-0.40000 µg/mL

Notes

-

Tested
Tested

Species

Human

Dilution info

0.25000-2.00000 µg/mL

Notes

Fixation/Permeabilization: PFA/Triton X-100.

Target data

Function

Isoform GANPAs a component of the TREX-2 complex, involved in the export of mRNAs to the cytoplasm through the nuclear pores (PubMed:20005110, PubMed:20384790, PubMed:23591820, PubMed:22307388). Through the acetylation of histones, affects the assembly of nucleosomes at immunoglobulin variable region genes and promotes the recruitment and positioning of transcription complex to favor DNA cytosine deaminase AICDA/AID targeting, hence promoting somatic hypermutations (PubMed:23652018).Isoform MCM3APBinds to and acetylates the replication protein MCM3. Plays a role in the initiation of DNA replication and participates in controls that ensure that DNA replication initiates only once per cell cycle (PubMed:11258703, PubMed:12226073). Through the acetylation of histones, affects the assembly of nucleosomes at immunoglobulin variable region genes and promotes the recruitment and positioning of transcription complex to favor DNA cytosine deaminase AICDA/AID targeting, hence promoting somatic hypermutations (PubMed:23652018).

Alternative names

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Rabbit Polyclonal GANP antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MCM3AP aa 300-450.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human MCM3AP aa 300-450. The exact immunogen used to generate this antibody is proprietary information. Database link O60318
Purification technique

Affinity purification Immunogen

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological function summary

The GANP protein plays an essential role in DNA replication and repair mechanisms. It contributes to immune responses by facilitating the maturation of antibody-producing B cells. GANP functions as a part of a larger protein complex often associating with other nucleic acid-binding proteins. Its activity supports somatic hypermutation and class switch recombination processes critical for the diversification of the antibody repertoire.

Activity summary

GANP also known as Germinal Center Associated Nuclear Protein and encoded by the MCM3AP gene is a protein with a molecular mass of approximately 210 kDa. GANP shows expression in various tissues most notably in germinal center B cells within lymphoid organs. It also appears in other cell types but at lower levels. The structure of GANP reflects its multifunctional role in cellular processes particularly in the context of immune function and cell cycle regulation.

Pathways

GANP integrates into the DNA replication pathway and the antigen receptor-mediated signaling pathway. In the DNA replication pathway GANP works alongside proteins like MCM3 and other components of the replication machinery to ensure accurate DNA synthesis. In the context of immune signaling GANP interacts with various signaling molecules enhancing adaptive immune responses through its regulatory functions.

Associated diseases and disorders

GANP involvement connects primarily to immunological diseases and certain cancers. Aberrations in GANP function or expression can associate with autoimmune disorders due to its impact on immune cell regulation. In cancers GANP expression has been observed in malignancies where it may contribute to unchecked cellular proliferation. The MCM3 protein a close functional associate of GANP highlights its role in pathways that when disrupted potentially lead to tumorigenesis and other immune-related pathologies.

Product promise

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2 product images

  • Western blot - Anti-GANP antibody (ab224434), expandable thumbnail

    Western blot - Anti-GANP antibody (ab224434)

    All lanes: Western blot - Anti-GANP antibody (AB224434) at 0.4 µg/mL

    All lanes: K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate

    Predicted band size: 218 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-GANP antibody (ab224434), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-GANP antibody (ab224434)

    Immunofluorescent analysis of U-251 MG (human brain glioma cell line) cells labeling GANP (green) in nuclear membrane and cytosol with ab224434 at 2 μg/ml.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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