Anti-GAP43 antibody [3H14] - Neuronal Marker

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GAP43 antibody [3H14] - Neuronal Marker (AB315198)

Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with ab315198, dilution 1 : 1,000, in red, and costained with chicken pAb to MAP2, CPCA-MAP2, dilution 1 : 10,000, in green. The blue is DAPI staining of nuclear DNA. GAP43 antibody labels protein expressed in the axonal membrane of the neuronal cells, while the MAP2 antibody stains dendrites and perikarya of neurons.

• WB

Supplier Data

Western blot - Anti-GAP43 antibody [3H14] - Neuronal Marker (AB315198)

Western blot analysis of different tissue and cell lysates using ab315198, dilution 1 : 5,000, in green : [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] C6 cells, [7] SH-SY5Y cells. The single band at the 43kDa mark corresponds to the GAP43 protein. The protein is expressed in rodent and human neurons and neuronal derived cells but not in C6 cells which are of glial origin.

All lanes:

Western blot - Anti-GAP43 antibody [3H14] - Neuronal Marker (ab315198) at 1/5000 dilution

Lane 2:

Rat brain tissue lysate

Lane 3:

Rat spinal cord tissue lysate

Lane 4:

Mouse brain tissue lysate

Lane 5:

Mouse spinal cord tissue lysate

Lane 6:

C6 cell lysate

Lane 7:

SH-SY5Y cell lysate

Observed band size: 43 kDa

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