AB16053

Anti-GAP43 antibody - Neuronal Marker

Name: Anti-GAP43 antibody - Neuronal Marker (ab16053) | Abcam
Brand: Abcam Limited
SKU: AB16053
Price: 400 USD
Availability: InStock
Rating: 4 (13 reviews)

Lab Essentials
What is this?

(13 Reviews)

(94 Publications)

Rabbit Polyclonal GAP43 antibody. Suitable for WB, IHC (PFA fixed) and reacts with Mouse, Rat samples. Cited in 94 publications.

View Alternative Names

Neuromodulin, Axonal membrane protein GAP-43, Growth-associated protein 43, Protein F1

3 Images

Immunohistochemistry (PFA fixed) - Anti-GAP43 antibody - Neuronal Marker (AB16053)

IHC (PFA fixed)

Collaborator

Immunohistochemistry (PFA fixed) - Anti-GAP43 antibody - Neuronal Marker (AB16053)

Immunofluorescent staining obtained with ab16053 Rabbit polyclonal to GAP43 in rat dorsal root ganglion, 2 weeks after a spinal nerve injury. The staining is localised in the cytoplasm of some lesioned neurons (arrow in B) and in many axons (arrow heads in B). Image B (X40 objective) is a higher magnification of A (X20). The staining disappeared completely when the antibody was pre-incubated with the immunising (blocking) peptide (ab16379).

Protocol details : Rats were intracardially perfused with 4% paraformaldehyde, DRG tissue was post-fixed overnight in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. IHC free floating was performed on the fixed, frozen and cut (20μm sections) rat tissue. Primary antibody ab16053 was incubated overnight at toom temperature at 1/300. Secondary antibody Alexa fluor 488 was incubated at 1/1000 for 2 hours at room temperature.

This image is courtesy of Sophie Pezet, Univ London Kings Coll, United Kingdom

Western blot - Anti-GAP43 antibody - Neuronal Marker (AB16053)

All lanes:

Western blot - Anti-GAP43 antibody - Neuronal Marker (ab16053) at 1 µg/mL

All lanes:

Spinal Cord (Mouse) Tissue Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 24 kDa

Observed band size: 100 kDa,43 kDa,50 kDa

true

Exposure time: 8min

Project

Western blot - Anti-GAP43 antibody - Neuronal Marker (AB16053)

All lanes:

Western blot - Anti-GAP43 antibody - Neuronal Marker (ab16053) at 1 µg/mL

Lane 1:

spinal cord chung rat 1 at 20 µg

Lane 2:

spinal cord chung rat 2 at 20 µg

Lane 3:

spinal cord chung rat 3 at 20 µg

Secondary

All lanes:

Dako Goat polyclonal to Rabbit IgG (HRP) at 1/2000 dilution

Predicted band size: 24 kDa

Observed band size: 43 kDa,90 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

IHC (PFA fixed), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody does not perform well in IHC-P (abreview 21090, 21089)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCPFAfixed" : {"fullname" : "Immunohistochemistry (PFA fixed)", "shortname":"IHC (PFA fixed)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCPFAfixed-species-checked": "predicted", "IHCPFAfixed-species-dilution-info": "", "IHCPFAfixed-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCPFAfixed-species-checked": "guaranteed", "IHCPFAfixed-species-dilution-info": "", "IHCPFAfixed-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCPFAfixed-species-checked": "testedAndGuaranteed", "IHCPFAfixed-species-dilution-info": "", "IHCPFAfixed-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCPFAfixed-species-checked": "predicted", "IHCPFAfixed-species-dilution-info": "", "IHCPFAfixed-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GAP43 commonly called Growth Associated Protein 43 or neuronal marker is a protein with a molecular weight of approximately 25-50 kDa. Researchers identify it by various names including GAP-43 GAP43 anticuerpo or GAP43 neuronal marker. This protein exhibits a specific presence in the nervous system especially in axons of neurons. It has significant expression during neural development and regeneration indicating its role in growth and repair.

Biological function summary

GAP43 plays an important role in neurite outgrowth and synaptic plasticity. It functions as an important mediator of signal transduction in neurons. GAP43 does not act alone but integrates into a network of signaling pathways that guide axonal elongation and branching. It works closely with cytoskeletal elements influencing the structural dynamics needed for effective neuronal response and adaptation.

Pathways

GAP43 is vital in the regulation of the protein kinase C pathway and the cAMP pathway both essential for neuronal growth and differentiation. It closely interacts with proteins such as calmodulin which binds to GAP43 and impacts its function in those pathways. These interactions highlight its role in the modulation of neuronal growth and synaptic plasticity making it essential for neurons' response to environmental signals.

GAP43 associates with neurodegenerative diseases and cognitive disorders particularly Alzheimer's disease and schizophrenia. Changes in GAP43 expression levels correlate with the severity and progression of these conditions. Additionally GAP43 connects to other proteins implicated in such diseases like tau in Alzheimer's suggesting a multifaceted involvement in disease mechanisms. This makes it a target of interest for therapeutic research as modulating GAP43 activity might alter disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

This protein is associated with nerve growth. It is a major component of the motile 'growth cones' that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction (By similarity).

See full target information Gap43

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Publications (94)

Recent publications for all applications. Explore the full list and refine your search

Brain and behavior 15:e70413 PubMed40084394

2025

Resveratrol Alleviates the Prenatally Intermittent Hypoxia-Induced Cognitive Impairment in Male Offspring Mice Through Modulating the SIRT1/HIF-1α Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xun He,Ziwei Cao,Xinyi Chen,Jie Hu,Jiaxuan Li,Xinhui Jia,Juncang Wu,Xuechun Liu

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2409086 PubMed39804990

2025

TIA1-Mediated Stress Granules Promote the Neuroinflammation and Demyelination in Experimental Autoimmune Encephalomyelitis through Upregulating IL-31RA Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Hua,Lingting Jin,Zheyu Fang,Yiyun Weng,Yuan Zhang,Jingjing Zhang,Dewei Xie,Yang Tang,Siyu Guo,Yingying Huang,Yilin Dai,Jia Li,Zhihui Huang,Xu Zhang

Acta physiologica (Oxford, England) 241:e14279 PubMed39803764

2025

Left superior cervical ganglia lymph node mimicry and its role in rat ventricular arrhythmias following myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Qingxia Yu,Yan Li,Wenju Yan,Weizhong Han,Qian Liu,Junyi Zhang,Xiaolu Li,Yugen Shi,Yu Wang,Jie Yin,Suhua Yan

The Journal of neuroscience : the official journal of the Society for Neuroscience 44: PubMed39266301

2024

A miR-383-5p Signaling Hub Coordinates the Axon Regeneration Response to Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew A Hintermayer,Camille A Juźwik,Barbara Morquette,Elizabeth Hua,Julia Zhang,Sienna Drake,Shan Shan Shi,Isabel Rambaldi,Vamshi Vangoor,Jeroen Pasterkamp,Craig Moore,Alyson E Fournier

Brain and behavior 14:e3610 PubMed38945806

2024

Subsequent maternal sleep deprivation aggravates cognitive impairment by modulating hippocampal neuroinflammatory responses and synaptic function in maternal isoflurane-exposed offspring mice.

Applications

Unspecified application

Species

Unspecified reactive species

Meng-Ying Zhang,Ru-Meng Wei,Jun Jing,Shu-Ren Huang,Gao-Lin Qiu,Xiao-Qiong Xia,Yue-Ming Zhang,Yuan-Hai Li

Neuroscience bulletin 40:1230-1244 PubMed38592581

2024

Mertk Reduces Blood-Spinal Cord Barrier Permeability Through the Rhoa/Rock1/P-MLC Pathway After Spinal Cord Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Jiezhao Lin,Yuanfang Sun,Bin Xia,Yihan Wang,Changnan Xie,Jinfeng Wang,Jinwei Hu,Lixin Zhu

Journal of pharmaceutical analysis 14:86-99 PubMed38352945

2024

Hydralazine represses Fpn ubiquitination to rescue injured neurons via competitive binding to UBA52.

Applications

Unspecified application

Species

Unspecified reactive species

Shengyou Li,Xue Gao,Yi Zheng,Yujie Yang,Jianbo Gao,Dan Geng,Lingli Guo,Teng Ma,Yiming Hao,Bin Wei,Liangliang Huang,Yitao Wei,Bing Xia,Zhuojing Luo,Jinghui Huang

Journal of translational medicine 22:114 PubMed38287296

2024

Tirzepatide prevents neurodegeneration through multiple molecular pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Rosaria Anna Fontanella,Puja Ghosh,Ada Pesapane,Fatemeh Taktaz,Armando Puocci,Martina Franzese,Maria Federica Feliciano,Giovanni Tortorella,Lucia Scisciola,Eduardo Sommella,Concetta Ambrosino,Giuseppe Paolisso,Michelangela Barbieri

Frontiers in endocrinology 14:1286931 PubMed38189047

2024

Allopregnanolone pleiotropic action in neurons and astrocytes: calcium signaling as a unifying mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Tian Wang,Shuhua Chen,Zisu Mao,Yuan Shang,Roberta Diaz Brinton

BMC biology 21:235 PubMed37880634

2023

Unveiling adcyap1 as a protective factor linking pain and nerve regeneration through single-cell RNA sequencing of rat dorsal root ganglion neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Chen,Xi-Yin Zhang,Yu-Pu Wang,Yun-Jie Fu,Feng Cao,Yi-Nuo Xu,Jin-Ge Kong,Na-Xi Tian,Yu Xu,Yun Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GAP43 antibody - Neuronal Marker

Immunohistochemistry (PFA fixed) - Anti-GAP43 antibody - Neuronal Marker (AB16053)

Western blot - Anti-GAP43 antibody - Neuronal Marker (AB16053)

Western blot - Anti-GAP43 antibody - Neuronal Marker (AB16053)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (94)

Product promise