Rabbit Polyclonal GAP43 antibody. Suitable for WB, IHC (PFA fixed) and reacts with Mouse, Rat samples. Cited in 74 publications.
Images
Publications
Filter by
- BMC biology 21:2352023Unveiling adcyap1 as a protective factor linking pain and nerve regeneration through single-cell RNA sequencing of rat dorsal root ganglion neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQi Chen,Xi-Yin Zhang,Yu-Pu Wang,Yun-Jie Fu,Feng Cao,Yi-Nuo Xu,Jin-Ge Kong,Na-Xi Tian,Yu Xu,Yun WangPubMed 37880634
- International journal of molecular sciences 24:2023Involvement of IL-1β-Mediated Necroptosis in Neurodevelopment Impairment after Neonatal Sepsis in Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhimin Liao,Qing Zhu,Han HuangPubMed 37834141
- eNeuro 10:2023Nicotine Decreases Nerve Regeneration and Pain Behaviors via PTEN and Downstream Inflammation-Related Pathway in Two Rat Nerve Injury Models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYehong Fang,Tingkai Zhang,Ling Li,Shanshan Chen,Liangliang Wang,Jinsong Tang,Yanhui LiaoPubMed 37620149
- Neurochemical research 48:996-10082022GPR137 Inhibits Cell Proliferation and Promotes Neuronal Differentiation in the Neuro2a Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKensuke Iwasa,Anzu Yamagishi,Shinji Yamamoto,Chikara Haruta,Kei Maruyama,Keisuke YoshikawaPubMed 36436172
- Cell death & disease 13:7592022Astrocytic SARM1 promotes neuroinflammation and axonal demyelination in experimental autoimmune encephalomyelitis through inhibiting GDNF signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLingting Jin,Jingjing Zhang,Xin Hua,Xingxing Xu,Jia Li,Jiaojiao Wang,Mianxian Wang,Huitao Liu,Haoyu Qiu,Man Chen,Xu Zhang,Ying Wang,Zhihui HuangPubMed 36055989
- Neural regeneration research 18:657-6632022A hyaluronic acid granular hydrogel nerve guidance conduit promotes regeneration and functional recovery of injured sciatic nerves in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Yang,Chia-Chen Hsu,Ting-Ting Cao,Hua Ye,Jing Chen,Yun-Qing LiPubMed 36018191
- Evidence-based complementary and alternative medicine : eCAM 2022:13335452022Flavonoid Extract from Propolis Provides Cardioprotection following Myocardial Infarction by Activating PPAR-.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQian Wang,Rui Chen,Qian Tang,Jin-Ying Chen,Yan Wang,Dian-Jun Sui,Ai-Dong LiuPubMed 35928246
- The Journal of experimental medicine 219:2022Interleukin-4 receptor signaling modulates neuronal network activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNicholas Hanuscheck,Carine Thalman,Micaela Domingues,Samantha Schmaul,Muthuraman Muthuraman,Florian Hetsch,Manuela Ecker,Heiko Endle,Mohammadsaleh Oshaghi,Gianvito Martino,Tanja Kuhlmann,Katarzyna Bozek,Tim van Beers,Stefan Bittner,Jakob von Engelhardt,Johannes Vogt,Christina Francisca Vogelaar,Frauke ZippPubMed 35587822
- Stem cell reports 17:664-6772022YAP signaling in horizontal basal cells promotes the regeneration of olfactory epithelium after injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQian Wu,Xingxing Xu,Xuemeng Miao,Xiaomei Bao,Xiuchun Li,Ludan Xiang,Wei Wang,Siyu Du,Yi Lu,Xiwu Wang,Danlu Yang,Jingjing Zhang,Xiya Shen,Fayi Li,Sheng Lu,Yiren Fan,Shujie Xu,Zihao Chen,Ying Wang,Honglin Teng,Zhihui HuangPubMed 35148842
- Computational and mathematical methods in medicine 2021:85224172021Activation of Neural Modeling-Related Genes in the Heart of Mice after Gamma Irradiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhiyong Liu,Jing Liu,Dan Hu,Juanjuan Du,Donglu Liu,Ximin Wang,Jiandong Zhang,Yinglong HouPubMed 35003326
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Consider this alternative
Reactivity data
Select an application| WB | IHC-P | IHC (PFA fixed) | |
|---|---|---|---|
| Human | Predicted | Not recommended | Predicted |
| Mouse | Tested | Not recommended | Expected |
| Rat | Tested | Not recommended | Tested |
| Chimpanzee | Predicted | Not recommended | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Rat | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Chimpanzee | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Chimpanzee | Dilution info - | Notes - |
IHC (PFA fixed)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Chimpanzee | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
This protein is associated with nerve growth. It is a major component of the motile 'growth cones' that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction (By similarity).
Alternative names
Neuromodulin, Axonal membrane protein GAP-43, Growth-associated protein 43, Protein F1
Recommended products
Rabbit Polyclonal GAP43 antibody. Suitable for WB, IHC (PFA fixed) and reacts with Mouse, Rat samples. Cited in 74 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
This antibody does not perform well in IHC-P (abreview 21090, 21089)
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GAP43 commonly called Growth Associated Protein 43 or neuronal marker is a protein with a molecular weight of approximately 25-50 kDa. Researchers identify it by various names including GAP-43 GAP43 anticuerpo or GAP43 neuronal marker. This protein exhibits a specific presence in the nervous system especially in axons of neurons. It has significant expression during neural development and regeneration indicating its role in growth and repair.
- Biological function summary
GAP43 plays an important role in neurite outgrowth and synaptic plasticity. It functions as an important mediator of signal transduction in neurons. GAP43 does not act alone but integrates into a network of signaling pathways that guide axonal elongation and branching. It works closely with cytoskeletal elements influencing the structural dynamics needed for effective neuronal response and adaptation.
- Pathways
GAP43 is vital in the regulation of the protein kinase C pathway and the cAMP pathway both essential for neuronal growth and differentiation. It closely interacts with proteins such as calmodulin which binds to GAP43 and impacts its function in those pathways. These interactions highlight its role in the modulation of neuronal growth and synaptic plasticity making it essential for neurons' response to environmental signals.
- Associated diseases and disorders
GAP43 associates with neurodegenerative diseases and cognitive disorders particularly Alzheimer's disease and schizophrenia. Changes in GAP43 expression levels correlate with the severity and progression of these conditions. Additionally GAP43 connects to other proteins implicated in such diseases like tau in Alzheimer's suggesting a multifaceted involvement in disease mechanisms. This makes it a target of interest for therapeutic research as modulating GAP43 activity might alter disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-GAP43 antibody - Neuronal Marker (ab16053)
All lanes: Western blot - Anti-GAP43 antibody - Neuronal Marker (ab16053) at 1 µg/mL
Lane 1: spinal cord chung rat 1 at 20 µg
Lane 2: spinal cord chung rat 2 at 20 µg
Lane 3: spinal cord chung rat 3 at 20 µg
SecondaryAll lanes: Dako Goat polyclonal to Rabbit IgG (HRP) at 1/2000 dilution
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 43 kDa, 90 kDa
This image is courtesy of Sophie Pezet, Univ London Kings Coll, United KingdomImmunohistochemistry (PFA fixed) - Anti-GAP43 antibody - Neuronal Marker (ab16053)
Immunofluorescent staining obtained with ab16053 Rabbit polyclonal to GAP43 in rat dorsal root ganglion, 2 weeks after a spinal nerve injury. The staining is localised in the cytoplasm of some lesioned neurons (arrow in B) and in many axons (arrow heads in B). Image B (X40 objective) is a higher magnification of A (X20). The staining disappeared completely when the antibody was pre-incubated with the immunising (blocking) peptide (ab16379).
Protocol details: Rats were intracardially perfused with 4% paraformaldehyde, DRG tissue was post-fixed overnight in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. IHC free floating was performed on the fixed, frozen and cut (20μm sections) rat tissue. Primary antibody ab16053 was incubated overnight at toom temperature at 1/300. Secondary antibody Alexa fluor 488 was incubated at 1/1000 for 2 hours at room temperature.
Western blot - Anti-GAP43 antibody - Neuronal Marker (ab16053)
All lanes: Western blot - Anti-GAP43 antibody - Neuronal Marker (ab16053) at 1 µg/mL
All lanes: Spinal Cord (Mouse) Tissue Lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 100 kDa, 43 kDa, 50 kDa
Exposure time: 8min
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com