Rabbit Recombinant Monoclonal GAP43 phospho S41 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-GAP43 (phospho S41) antibody [EPR1854(2)] - BSA and Azide free (AB249404), expandable thumbnail](https://content.abcam.com/products/images/gap43-phospho-s41-antibody-epr18542-bsa-and-azide-free-ab249404--western-blot-img76748.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GAP43 (phospho S41) antibody [EPR1854(2)] - BSA and Azide free (AB249404)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Predicted | Not recommended | Not recommended |
| Rat | Not recommended | Predicted | Not recommended | Not recommended |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
This protein is associated with nerve growth. It is a major component of the motile 'growth cones' that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction.
Alternative names
Neuromodulin, Axonal membrane protein GAP-43, Growth-associated protein 43, Neural phosphoprotein B-50, pp46, GAP43
Recommended products
Rabbit Recombinant Monoclonal GAP43 phospho S41 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR1854(2)
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249404 is the carrier-free version of Anti-GAP43 (phospho S41) antibody [EPR1854(2)] ab167162.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GAP43 commonly called Growth Associated Protein 43 or neuronal marker is a protein with a molecular weight of approximately 25-50 kDa. Researchers identify it by various names including GAP-43 GAP43 anticuerpo or GAP43 neuronal marker. This protein exhibits a specific presence in the nervous system especially in axons of neurons. It has significant expression during neural development and regeneration indicating its role in growth and repair.
- Biological function summary
GAP43 plays an important role in neurite outgrowth and synaptic plasticity. It functions as an important mediator of signal transduction in neurons. GAP43 does not act alone but integrates into a network of signaling pathways that guide axonal elongation and branching. It works closely with cytoskeletal elements influencing the structural dynamics needed for effective neuronal response and adaptation.
- Pathways
GAP43 is vital in the regulation of the protein kinase C pathway and the cAMP pathway both essential for neuronal growth and differentiation. It closely interacts with proteins such as calmodulin which binds to GAP43 and impacts its function in those pathways. These interactions highlight its role in the modulation of neuronal growth and synaptic plasticity making it essential for neurons' response to environmental signals.
- Associated diseases and disorders
GAP43 associates with neurodegenerative diseases and cognitive disorders particularly Alzheimer's disease and schizophrenia. Changes in GAP43 expression levels correlate with the severity and progression of these conditions. Additionally GAP43 connects to other proteins implicated in such diseases like tau in Alzheimer's suggesting a multifaceted involvement in disease mechanisms. This makes it a target of interest for therapeutic research as modulating GAP43 activity might alter disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-GAP43 (phospho S41) antibody [EPR1854(2)] - BSA and Azide free (ab249404), expandable thumbnail](https://content.abcam.com/products/images/gap43-phospho-s41-antibody-epr18542-bsa-and-azide-free-ab249404--western-blot-img76748.jpg "Western blot - Anti-GAP43 (phospho S41) antibody [EPR1854(2)] - BSA and Azide free (ab249404)")
Western blot - Anti-GAP43 (phospho S41) antibody [EPR1854(2)] - BSA and Azide free (ab249404)
This data was developed using Anti-GAP43 (phospho S41) antibody [EPR1854(2)] ab167162, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-GAP43 (phospho S41) antibody [EPR1854(2)] (Anti-GAP43 (phospho S41) antibody [EPR1854(2)] ab167162) at 1/1000 dilution
Lane 1: Untreated SH-SY5Y cell lysates at 10 µg
Lane 2: SH-SY5Y cell lysates treated with Okadaic Acid and Calyculin A. at 10 µg
Predicted band size: 24 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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