Skip to main content

Mouse Monoclonal GAPDH antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human GAPDH aa 150-200.


Images

Western blot - Anti-GAPDH antibody [12G11] - Loading Control (AB226408), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [12G11] - Loading Control (AB226408), expandable thumbnail
  • Western blot - Anti-GAPDH antibody [12G11] - Loading Control (AB226408), expandable thumbnail
  • Western blot - Anti-GAPDH antibody [12G11] - Loading Control (AB226408), expandable thumbnail
  • Immunocytochemistry - Anti-GAPDH antibody [12G11] - Loading Control (AB226408), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Synthetic Peptide within Human GAPDH aa 150-200. The exact immunogen used to generate this antibody is proprietary information. Database link P04406

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IF
Human
Tested
Tested
Tested
Mouse
Tested
Expected
Expected
Rat
Predicted
Predicted
Predicted
Carp
Predicted
Predicted
Predicted
Chicken
Predicted
Predicted
Predicted
Cow
Predicted
Predicted
Predicted
Guinea pig
Predicted
Predicted
Predicted
Nile tilapia
Predicted
Predicted
Predicted
Orangutan
Predicted
Predicted
Predicted
Pig
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Turkey
Predicted
Predicted
Predicted
Zebrafish
Predicted
Predicted
Predicted

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Predicted
Predicted

Species

Rat, Sheep, Rabbit, Chicken, Guinea pig, Turkey, Pig, Carp, Zebrafish, Orangutan, Nile tilapia, Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/20.00000 - 1/50.00000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat, Sheep, Rabbit, Chicken, Guinea pig, Turkey, Pig, Carp, Zebrafish, Orangutan, Nile tilapia, Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100 - 1/500

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat, Sheep, Rabbit, Chicken, Guinea pig, Turkey, Pig, Carp, Zebrafish, Orangutan, Nile tilapia, Cow

Dilution info

-

Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively (PubMed:3170585, PubMed:11724794). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate (PubMed:3170585, PubMed:11724794). Modulates the organization and assembly of the cytoskeleton (By similarity). Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes (PubMed:23071094). Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (PubMed:23071094). Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (PubMed:23332158, PubMed:27387501). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis (By similarity). Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity).

Alternative names

Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH, OK/SW-cl.12, CDABP0047, GAPD, GAPDH

Recommended products

Mouse Monoclonal GAPDH antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human GAPDH aa 150-200.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Synthetic Peptide within Human GAPDH aa 150-200. The exact immunogen used to generate this antibody is proprietary information. Database link P04406
Clone number

12G11

Purification technique

Affinity purification Immunogen

Concentration
Loading...
Purification notes

ab226408 was affinity purified using an epitope specific to GAPDH immobilized on solid support.Purified from TCS.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Glyceraldehyde-3-phosphate dehydrogenase commonly known as GAPDH is an enzyme involved in glycolysis. Its molecular weight (MW) is approximately 36 kDa. The protein is expressed ubiquitously in almost all tissues reflecting its essential role in energy production. GAPDH catalyzes the sixth step of glycolysis converting glyceraldehyde-3-phosphate into 13-bisphosphoglycerate. Due to its stable expression researchers often use GAPDH as a loading control in western blot experiments.

Biological function summary

GAPDH serves important metabolic functions beyond its enzymatic role in glycolysis. It functions as part of a multi-enzyme complex within the cytoplasm which facilitates efficient substrate channeling during glycolysis. Additionally GAPDH has non-glycolytic roles including involvement in nuclear processes like RNA export and DNA repair. Its ubiquitous presence across different cellular compartments indicates its multiple functions beyond metabolic pathways.

Pathways

GAPDH integrates into significant cellular functions like the glycolytic pathway and apoptotic pathways. In glycolysis GAPDH collaborates with enzymes like phosphoglycerate kinase forming a cohesive link in the energy conversion chain. Its participation in apoptotic pathways highlights GAPDH's involvement in cellular death processes interacting with proteins like Bcl-2 to influence apoptosis progression. These roles reinforce its presence in central metabolic and regulatory pathways.

Associated diseases and disorders

GAPDH has associations with neurodegenerative diseases and cancer. In neurodegenerative disorders such as Alzheimer's disease GAPDH’s altered enzymatic activity is frequently observed influencing cellular energy homeostasis. Moreover overexpression or aberrant regulation of GAPDH relates to cancer cell proliferation and metastasis implicating proteins like p53 in these pathways. The diverse functions and interactions of GAPDH emphasize its importance in both normal cellular function and disease states.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-GAPDH antibody [12G11] - Loading Control (ab226408), expandable thumbnail

    Western blot - Anti-GAPDH antibody [12G11] - Loading Control (ab226408)

    Cell lysates were prepared using NETN lysis buffer.

    All lanes: Western blot - Anti-GAPDH antibody [12G11] - Loading Control (ab226408) at 1/1000 dilution

    Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

    Lane 2: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

    Lane 3: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

    Lane 4: TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

    Lane 5: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 36 kDa

    Exposure time: 3min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [12G11] - Loading Control (ab226408), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [12G11] - Loading Control (ab226408)

    Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for GAPDH using ab226408 at 1/20 dilution in immunohistochemical analysis. Detection: DAB staining.

  • Western blot - Anti-GAPDH antibody [12G11] - Loading Control (ab226408), expandable thumbnail

    Western blot - Anti-GAPDH antibody [12G11] - Loading Control (ab226408)

    All lanes: Western blot - Anti-GAPDH antibody [12G11] - Loading Control (ab226408) at 1/1000 dilution

    Lane 1: EL4 whole cell lysate at 15 µg

    Lane 2: mIMCD-3 whole cell lysate at 15 µg

    Lane 3: RenCa whole cell lysate at 15 µg

    Lane 4: NIH 3T3 whole cell lysate at 15 µg

    Lane 5: TCMK-1 whole cell lysate at 15 µg

    Lane 6: C2C12 whole cell lysate at 15 µg

    Lane 7: A20 whole cell lysate at 15 µg

    Lane 8: Ba/F3 whole cell lysate at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051)

    Developed using the ECL technique.

    Predicted band size: 35 kDa

    Observed band size: 37 kDa

    Exposure time: 10s

  • Western blot - Anti-GAPDH antibody [12G11] - Loading Control (ab226408), expandable thumbnail

    Western blot - Anti-GAPDH antibody [12G11] - Loading Control (ab226408)

    All lanes: Western blot - Anti-GAPDH antibody [12G11] - Loading Control (ab226408) at 1/1000 dilution

    Lane 1: HeLa whole cell lysate at 50 µg

    Lane 2: Jurkat whole cell lysate at 50 µg

    Lane 3: RKO whole cell lysate at 50 µg

    Lane 4: U2OS whole cell lysate at 50 µg

    Lane 5: GaMG whole cell lysate at 50 µg

    Lane 6: LNCaP whole cell lysate at 50 µg

    Lane 7: MCF-7 whole cell lysate at 50 µg

    Lane 8: K-562 whole cell lysate at 50 µg

    Lane 9: HEK293T whole cell lysate at 50 µg

    Lane 10: Hep-G2 whole cell lysate at 50 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051)

    Developed using the ECL technique.

    Predicted band size: 36 kDa

    Observed band size: 36 kDa

    Exposure time: 1s

  • Immunocytochemistry - Anti-GAPDH antibody [12G11] - Loading Control (ab226408), expandable thumbnail

    Immunocytochemistry - Anti-GAPDH antibody [12G11] - Loading Control (ab226408)

    Immunocytochemical analysis of Formalin-Fixed Paraffin-Embedded HEK293Tcells labeling human GAPDH with ab226408 followed by a HRP-conjugated goat anti-rabbit IgG as secondary. Substrate: DAB. Nuclear and cytoplasmic staining on OVCAR-3 cells is observed. Counter stained with hematoxylin. Epitope retrieval with citrate buffer pH 6.0 for 20 minutes is recommended.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [12G11] - Loading Control (ab226408), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [12G11] - Loading Control (ab226408)

    Immunohistochemical analysis of Formalin-Fixed Paraffin-Embedded ovarian carcinoma labeling human GAPDH with ab226408, followed by a HRP-conjugated goat anti-rabbit IgG as secondary. Substrate: DAB. Nuclear and cytoplasmic staining on ovarian carcinoma is observed. Counter stained with hematoxylin. Epitope retrieval with citrate buffer pH 6.0 for 20 minutes is recommended.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com