Mouse Monoclonal GAPDH antibody. Suitable for IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human GAPDH.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [4G5] - Loading Control (AB189095), expandable thumbnail](https://content.abcam.com/products/images/gapdh-antibody-4g5-loading-control-ab189095--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img77841.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [4G5] - Loading Control (AB189095)")
Publications
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- Nature communications 13:16362022Filopodia rotate and coil by actively generating twist in their actin shaft.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNatascha Leijnse,Younes Farhangi Barooji,Mohammad Reza Arastoo,Stine Lauritzen Sønder,Bram Verhagen,Lena Wullkopf,Janine Terra Erler,Szabolcs Semsey,Jesper Nylandsted,Lene Broeng Oddershede,Amin Doostmohammadi,Poul Martin BendixPubMed 35347113
- Science advances 7:2021Restructuring of the plasma membrane upon damage by LC3-associated macropinocytosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStine Lauritzen Sønder,Swantje Christin Häger,Anne Sofie Busk Heitmann,Lisa B Frankel,Catarina Dias,Adam Cohen Simonsen,Jesper NylandstedPubMed 34215587
- PloS one 14:e02201252019Cell-autonomous and non-cell autonomous effects of neuronal BIN1 loss in vivo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKathleen M McAvoy et. al.PubMed 31408457
- Scientific reports 9:94772019BIN1 favors the spreading of Tau via extracellular vesicles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrea Crotti et. al.PubMed 31263146
- Scientific reports 9:67262019Annexin A7 is required for ESCRT III-mediated plasma membrane repair.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStine Lauritzen Sønder et. al.PubMed 31040365
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Native Full Length Protein corresponding to Human GAPDH. Database link P04406
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Associated Products
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6 products for Alternative Product
Target data
Function
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively (PubMed:11724794, PubMed:3170585). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate (PubMed:11724794, PubMed:3170585). Modulates the organization and assembly of the cytoskeleton (By similarity). Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes (PubMed:23071094). Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (PubMed:23071094). Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (PubMed:23332158, PubMed:27387501). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis (By similarity). Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity).
Alternative names
GAPD, CDABP0047, OK/SW-cl.12, GAPDH, Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH
Recommended products
Mouse Monoclonal GAPDH antibody. Suitable for IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human GAPDH.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Native Full Length Protein corresponding to Human GAPDH. Database link P04406
- Clone number
- 4G5
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Glyceraldehyde-3-phosphate dehydrogenase commonly known as GAPDH is an enzyme involved in glycolysis. Its molecular weight (MW) is approximately 36 kDa. The protein is expressed ubiquitously in almost all tissues reflecting its essential role in energy production. GAPDH catalyzes the sixth step of glycolysis converting glyceraldehyde-3-phosphate into 13-bisphosphoglycerate. Due to its stable expression researchers often use GAPDH as a loading control in western blot experiments.
- Biological function summary
GAPDH serves important metabolic functions beyond its enzymatic role in glycolysis. It functions as part of a multi-enzyme complex within the cytoplasm which facilitates efficient substrate channeling during glycolysis. Additionally GAPDH has non-glycolytic roles including involvement in nuclear processes like RNA export and DNA repair. Its ubiquitous presence across different cellular compartments indicates its multiple functions beyond metabolic pathways.
- Pathways
GAPDH integrates into significant cellular functions like the glycolytic pathway and apoptotic pathways. In glycolysis GAPDH collaborates with enzymes like phosphoglycerate kinase forming a cohesive link in the energy conversion chain. Its participation in apoptotic pathways highlights GAPDH's involvement in cellular death processes interacting with proteins like Bcl-2 to influence apoptosis progression. These roles reinforce its presence in central metabolic and regulatory pathways.
- Associated diseases and disorders
GAPDH has associations with neurodegenerative diseases and cancer. In neurodegenerative disorders such as Alzheimer's disease GAPDH’s altered enzymatic activity is frequently observed influencing cellular energy homeostasis. Moreover overexpression or aberrant regulation of GAPDH relates to cancer cell proliferation and metastasis implicating proteins like p53 in these pathways. The diverse functions and interactions of GAPDH emphasize its importance in both normal cellular function and disease states.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [4G5] - Loading Control (ab189095), expandable thumbnail](https://content.abcam.com/products/images/gapdh-antibody-4g5-loading-control-ab189095--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img77841.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [4G5] - Loading Control (ab189095)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [4G5] - Loading Control (ab189095)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skin tissue labeling GAPDH with ab189095 at 10 μg/ml.
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