Rabbit Polyclonal GAPDH antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human GAPDH.
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Publications
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- Biochemistry and biophysics reports 36:1015422023Exosomes released from PD-L1 tumor associated macrophages promote peritoneal metastasis of epithelial ovarian cancer by up-regulating T cell lipid metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Ma,Qianqian Cen,Qingzhu Wang,Li Liu,Jieru ZhouPubMed 37822876
- Animals : an open access journal from MDPI 13:2023Photoperiod Induces DNA Methylation Changes in the Melatonin Receptor 1A Gene in Ewes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoyun He,Wei Wang,Wei Sun,Mingxing ChuPubMed 37370427
- ACS omega 6:1465-14762021Comparative Study on 3D Printed Ti6Al4V Scaffolds with Surface Modifications Using Hydrothermal Treatment and Microarc Oxidation to Enhance Osteogenic Activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLeizhen Huang et. al.PubMed 33490806
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human GAPDH. The exact immunogen used to generate this antibody is proprietary information. Database link P04406
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
Associated Products
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6 products for Alternative Product
Target data
Function
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively (PubMed:11724794, PubMed:3170585). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate (PubMed:11724794, PubMed:3170585). Modulates the organization and assembly of the cytoskeleton (By similarity). Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes (PubMed:23071094). Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (PubMed:23071094). Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (PubMed:23332158, PubMed:27387501). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis (By similarity). Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity).
Alternative names
GAPD, CDABP0047, OK/SW-cl.12, GAPDH, Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH
Recommended products
Rabbit Polyclonal GAPDH antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human GAPDH.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human GAPDH. The exact immunogen used to generate this antibody is proprietary information. Database link P04406
- Purification technique
- Affinity purification Immunogen
- Specificity
Anti-GAPDH Antibody is directed against human GAPDH protein. A BLAST analysis was used to suggest that this antibody would react with GAPDH from a wide range of organisms, including most vertebrates and some yeast. Broad reactivity makes this antibody an excellent loading control.
- Concentration
- Purification notes
Anti-GAPDH Antibody is directed against human GAPDH protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Glyceraldehyde-3-phosphate dehydrogenase commonly known as GAPDH is an enzyme involved in glycolysis. Its molecular weight (MW) is approximately 36 kDa. The protein is expressed ubiquitously in almost all tissues reflecting its essential role in energy production. GAPDH catalyzes the sixth step of glycolysis converting glyceraldehyde-3-phosphate into 13-bisphosphoglycerate. Due to its stable expression researchers often use GAPDH as a loading control in western blot experiments.
- Biological function summary
GAPDH serves important metabolic functions beyond its enzymatic role in glycolysis. It functions as part of a multi-enzyme complex within the cytoplasm which facilitates efficient substrate channeling during glycolysis. Additionally GAPDH has non-glycolytic roles including involvement in nuclear processes like RNA export and DNA repair. Its ubiquitous presence across different cellular compartments indicates its multiple functions beyond metabolic pathways.
- Pathways
GAPDH integrates into significant cellular functions like the glycolytic pathway and apoptotic pathways. In glycolysis GAPDH collaborates with enzymes like phosphoglycerate kinase forming a cohesive link in the energy conversion chain. Its participation in apoptotic pathways highlights GAPDH's involvement in cellular death processes interacting with proteins like Bcl-2 to influence apoptosis progression. These roles reinforce its presence in central metabolic and regulatory pathways.
- Associated diseases and disorders
GAPDH has associations with neurodegenerative diseases and cancer. In neurodegenerative disorders such as Alzheimer's disease GAPDH’s altered enzymatic activity is frequently observed influencing cellular energy homeostasis. Moreover overexpression or aberrant regulation of GAPDH relates to cancer cell proliferation and metastasis implicating proteins like p53 in these pathways. The diverse functions and interactions of GAPDH emphasize its importance in both normal cellular function and disease states.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-GAPDH antibody (ab263962)
All lanes: Western blot - Anti-GAPDH antibody (ab263962) at 1/1000 dilution
Lane 1: NIH/3T3 (mouse embryo fibroblast cell line) lysate at 35 µg
Lane 2: HEK-293 (human epithelial cell line from embryonic kidney) lysate at 35 µg
Lane 3: Molt-4 (human lymphoblastic leukemia cell line) lysate at 35 µg
Lane 4: A549 (human lung carcinoma cell line) lysate at 35 µg
Lane 5: HeLa (human epithelial cell line from cervix adenocarcinoma) lysate at 35 µg
Lane 6: NIH/3T3 nuclear lysate at 35 µg
Lane 7: HeLa nuclear lysate at 35 µg
Lane 8: Jurkat (human T cell leukemia cell line from peripheral blood) lysate at 35 µg
SecondaryAll lanes: Peroxidase rabbit secondary antibody for 30 min at RT at 1/70000 dilution
Predicted band size: 36 kDa
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