Anti-GAPDH antibody [EPR16884] - Loading Control (ab181603) is a rabbit monoclonal antibody that is used to detect GAPDH in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF. Suitable for African green monkey, Chicken, Cow, Dog, Human, Mouse, Rat samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
-Over 200 publications
Images
![Western blot - Anti-GAPDH antibody [EPR16884] - Loading Control (AB181603), expandable thumbnail](https://content.abcam.com/products/images/gapdh-antibody-epr16884-loading-control-ab181603--western-blot-img103019.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GAPDH antibody [EPR16884] - Loading Control (AB181603)")
![Western blot - Anti-GAPDH antibody [EPR16884] - Loading Control (AB181603), expandable thumbnail](https://content.abcam.com/products/images/gapdh-antibody-epr16884-loading-control-ab181603--western-blot-img65499.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GAPDH antibody [EPR16884] - Loading Control (AB181603)")
![Western blot - Anti-GAPDH antibody [EPR16884] - Loading Control (AB181603), expandable thumbnail](https://content.abcam.com/products/images/gapdh-antibody-epr16884-loading-control-ab181603--western-blot-img111911.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GAPDH antibody [EPR16884] - Loading Control (AB181603)")
![Western blot - Anti-GAPDH antibody [EPR16884] - Loading Control (AB181603), expandable thumbnail](https://content.abcam.com/products/images/gapdh-antibody-epr16884-loading-control-ab181603--western-blot-img106081.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GAPDH antibody [EPR16884] - Loading Control (AB181603)")
![Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody [EPR16884] - Loading Control (AB181603), expandable thumbnail](https://content.abcam.com/products/images/gapdh-antibody-epr16884-loading-control-ab181603--immunocytochemistry-immunofluorescence-img14153.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody [EPR16884] - Loading Control (AB181603)")
Publications
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- International journal of molecular sciences 24:2023A Strainer-Based Platform for the Collection and Immunolabeling of Porcine Epidemic Diarrhea Virus-Infected Porcine Intestinal Organoid.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYinju Liu,Jinlong Tan,Nianzhang Zhang,Wenhui Li,Baoquan FuPubMed 37958655
- BMC complementary medicine and therapies 23:3812023Afzelin induces immunogenic cell death against lung cancer by targeting NQO2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLei Xia,Xiaoqing Xu,Meijun Li,Xinyue Zhang,Fang CaoPubMed 37891619
- Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 20:1820-18342023Persistent NRG1 Type III Overexpression in Spinal Motor Neurons Has No Therapeutic Effect on ALS-Related Pathology in SOD1 Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSara Hernández,Sara Salvany,Anna Casanovas,Lídia Piedrafita,M Clara Soto-Bernardini,Olga Tarabal,Alba Blasco,Sílvia Gras,Alaó Gatius,Markus H Schwab,Jordi Calderó,Josep E EsquerdaPubMed 37733208
- Investigative ophthalmology & visual science 64:342023Attenuation of Microglial Activation and Pyroptosis by Inhibition of P2X7 Pathway Promotes Photoreceptor Survival in Experimental Retinal Detachment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesManjing Cao,Xinting Huang,Jingling Zou,Yingqian Peng,Yanbing Wang,Xichen Zheng,Luosheng Tang,Lusi ZhangPubMed 37351878
- Science signaling 16:eabo48632023Neuropilin-1 interacts with VE-cadherin and TGFBR2 to stabilize adherens junctions and prevent activation of endothelium under flow.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEmy Bosseboeuf,Anissa Chikh,Ahmed Bey Chaker,Tom P Mitchell,Dhilakshani Vignaraja,Ridhi Rajendrakumar,Rayomand S Khambata,Thomas D Nightingale,Justin C Mason,Anna M Randi,Amrita Ahluwalia,Claudio RaimondiPubMed 37220183
- Development (Cambridge, England) 150:2023Stretch regulates alveologenesis and homeostasis via mesenchymal Gαq/11-mediated TGFβ2 activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmanda T Goodwin,Alison E John,Chitra Joseph,Anthony Habgood,Amanda L Tatler,Katalin Susztak,Matthew Palmer,Stefan Offermanns,Neil C Henderson,R Gisli JenkinsPubMed 37102682
- Immunity, inflammation and disease 11:e8192023Mmu_circ_0000037 inhibits the progression of acute pancreatitis by miR-92a-3p/Pias1 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHua Chen,Jun Tu,Lei He,Ning Gao,Weiqiang YangPubMed 37102653
- Frontiers in psychiatry 14:11603412023Circulating plasma and exosome levels of the miR-320 family as a non-invasive biomarker for methamphetamine use disorder.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenjin Xu,Qingxiao Hong,Yun Zhou,Xiaoyu Chen,Longhui Li,Majie Wang,Weisheng Chen,Xiaohu Xie,Dingding Zhuang,Miaojun Lai,Wenhua Zhou,Huifen LiuPubMed 37181871
- Molecules (Basel, Switzerland) 28:2023L. Alleviates Myocardial Ischemia-Induced Injury via Activation of MAPK/ERK Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChangli Fu,Mingjin Wang,Yuan Lu,Jie Pan,Yueting Li,Yongjun Li,Yonglin Wang,Aimin Wang,Yong Huang,Jia Sun,Chunhua LiuPubMed 37175097
- Cancer biomarkers : section A of Disease markers 37:39-522023Hsa_circRNA_001859 regulates pancreatic cancer progression and epithelial-mesenchymal transition through the miR-21-5p/SLC38A2 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiang Li,Nan Wang,Jun Wang,Jiangang LiPubMed 37005877
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected | Tested |
| Rat | Tested | Expected | Expected | Tested |
| African green monkey | Tested | Expected | Expected | Expected |
| Chicken | Tested | Expected | Expected | Expected |
| Cow | Tested | Expected | Expected | Expected |
| Dog | Tested | Expected | Expected | Expected |
| Monkey | Predicted | Predicted | Predicted | Predicted |
| Vervet monkey | Not recommended | Expected | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/10000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/10000 | Notes - |
Species Cow | Dilution info 1/10000 | Notes - |
Species Chicken | Dilution info 1/10000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Dog | Dilution info 1/10000 | Notes - |
Species Human | Dilution info 1/10000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species African green monkey | Dilution info 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Monkey | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Vervet monkey | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Dog | Dilution info - | Notes - |
Species African green monkey | Dilution info - | Notes - |
Species Mouse | Dilution info 1/250 | Notes - |
Species Rat | Dilution info 1/250 | Notes - |
Species Chicken | Dilution info 1/250 | Notes - |
Species Cow | Dilution info 1/250 | Notes - |
Species Vervet monkey | Dilution info 1/250 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Monkey | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species African green monkey | Dilution info - | Notes - |
Species Mouse | Dilution info 1/200 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Rat | Dilution info 1/200 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Chicken | Dilution info 1/200 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Cow | Dilution info 1/200 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Dog | Dilution info 1/200 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Vervet monkey | Dilution info 1/200 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Monkey | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species African green monkey | Dilution info - | Notes - |
Species Chicken | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Cow | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Dog | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Vervet monkey | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Monkey | Dilution info - | Notes - |
Associated Products
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Target data
Function
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively (PubMed:11724794, PubMed:3170585). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate (PubMed:11724794, PubMed:3170585). Modulates the organization and assembly of the cytoskeleton (By similarity). Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes (PubMed:23071094). Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (PubMed:23071094). Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (PubMed:23332158, PubMed:27387501). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis (By similarity). Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity).
Alternative names
GAPD, CDABP0047, OK/SW-cl.12, GAPDH, Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH
Recommended products
Anti-GAPDH antibody [EPR16884] - Loading Control (ab181603) is a rabbit monoclonal antibody that is used to detect GAPDH in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF. Suitable for African green monkey, Chicken, Cow, Dog, Human, Mouse, Rat samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
-Over 200 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR16884
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-GAPDH antibody [EPR16884] - Loading Control (ab181603) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), in African green monkey, Chicken, Cow, Dog, Human, Mouse, Rat samples.
What is the molecular weight of GAPDH?
Anti-GAPDH [EPR16884] - Loading Control (ab181603) is often used as a loading control in Western blot. It specifically detects a band for GAPDH (UniProt: P16858) at a molecular weight of 36kDa.
Recommended positive controls
WB: HeLa, MDBK, COS-1, MDCK, UMNSAH/DF-1, Jurkat, C6 and NIH/3T3 whole cell lysates; Mouse brain and heart lysates; Rat brain, heart, kidney and spleen lysates; Human fetal brain, heart and kidney lysates.IHC-P: Human transitional cell carcinoma of bladder, Mouse spleen and Rat spleen tissues. ICC/IF: HeLa cells. Flow: Jurkat cells.
Trusted by the scientific community
Anti-GAPDH [EPR16884] - Loading Control (ab181603) was first used in a scientific publication in 2014 and has been cited over 200 times in peer-reviewed journals.
Reviewed by scientists
Anti-GAPDH [EPR16884] - Loading Control (ab181603) has over 5 independent reviews from customers.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.Other related products
We have a range of other formats of antibody clone [EPR16884] also available for your convenience:
ab181603, Carrier free - Anti-GAPDH antibody [EPR16884] - BSA and Azide free ab199554, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-GAPDH antibody [EPR16884] ab204480, HRP - HRP Anti-GAPDH antibody [EPR16884] - Loading Control ab204481, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-GAPDH antibody [EPR16884] ab206373, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-GAPDH antibody [EPR16884] ab206629, PE - PE Anti-GAPDH antibody [EPR16884] ab209037
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Glyceraldehyde-3-phosphate dehydrogenase commonly known as GAPDH is an enzyme involved in glycolysis. Its molecular weight (MW) is approximately 36 kDa. The protein is expressed ubiquitously in almost all tissues reflecting its essential role in energy production. GAPDH catalyzes the sixth step of glycolysis converting glyceraldehyde-3-phosphate into 13-bisphosphoglycerate. Due to its stable expression researchers often use GAPDH as a loading control in western blot experiments.
- Biological function summary
GAPDH serves important metabolic functions beyond its enzymatic role in glycolysis. It functions as part of a multi-enzyme complex within the cytoplasm which facilitates efficient substrate channeling during glycolysis. Additionally GAPDH has non-glycolytic roles including involvement in nuclear processes like RNA export and DNA repair. Its ubiquitous presence across different cellular compartments indicates its multiple functions beyond metabolic pathways.
- Pathways
GAPDH integrates into significant cellular functions like the glycolytic pathway and apoptotic pathways. In glycolysis GAPDH collaborates with enzymes like phosphoglycerate kinase forming a cohesive link in the energy conversion chain. Its participation in apoptotic pathways highlights GAPDH's involvement in cellular death processes interacting with proteins like Bcl-2 to influence apoptosis progression. These roles reinforce its presence in central metabolic and regulatory pathways.
- Associated diseases and disorders
GAPDH has associations with neurodegenerative diseases and cancer. In neurodegenerative disorders such as Alzheimer's disease GAPDH’s altered enzymatic activity is frequently observed influencing cellular energy homeostasis. Moreover overexpression or aberrant regulation of GAPDH relates to cancer cell proliferation and metastasis implicating proteins like p53 in these pathways. The diverse functions and interactions of GAPDH emphasize its importance in both normal cellular function and disease states.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
9 product images
![Western blot - Anti-GAPDH antibody [EPR16884] - Loading Control (ab181603), expandable thumbnail](https://content.abcam.com/products/images/gapdh-antibody-epr16884-loading-control-ab181603--western-blot-img103019.jpg "Western blot - Anti-GAPDH antibody [EPR16884] - Loading Control (ab181603)")
Western blot - Anti-GAPDH antibody [EPR16884] - Loading Control (ab181603)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-GAPDH antibody [EPR16884] - Loading Control (ab181603) at 1/10000 dilution
Lane 1: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates at 20 µg
Lane 2: MDBK (Bovine kidney cell line) whole cell lysates at 20 µg
Lane 3: COS-1 (African green monkey kidney fibroblast-like cell line) whole cell lysates at 20 µg
Lane 4: MDCK (Canine kidney cell line) whole cell lysates at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
![Western blot - Anti-GAPDH antibody [EPR16884] - Loading Control (ab181603), expandable thumbnail](https://content.abcam.com/products/images/gapdh-antibody-epr16884-loading-control-ab181603--western-blot-img65499.jpg "Western blot - Anti-GAPDH antibody [EPR16884] - Loading Control (ab181603)")
Western blot - Anti-GAPDH antibody [EPR16884] - Loading Control (ab181603)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-GAPDH antibody [EPR16884] - Loading Control (ab181603) at 1/50000 dilution
Lane 1: UMNSAH/DF-1 (Transformed chicken embyronic fibroblast cells) whole cell lysates at 20 µg
Lane 2: Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
![Western blot - Anti-GAPDH antibody [EPR16884] - Loading Control (ab181603), expandable thumbnail](https://content.abcam.com/products/images/gapdh-antibody-epr16884-loading-control-ab181603--western-blot-img111911.jpg "Western blot - Anti-GAPDH antibody [EPR16884] - Loading Control (ab181603)")
Western blot - Anti-GAPDH antibody [EPR16884] - Loading Control (ab181603)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-GAPDH antibody [EPR16884] - Loading Control (ab181603) at 1/10000 dilution
Lane 1: Mouse brain lysate at 10 µg
Lane 2: Mouse heart lysate at 10 µg
Lane 3: Rat brain lysate at 10 µg
Lane 4: Rat heart lysate at 10 µg
Lane 5: Rat kidney lysate at 10 µg
Lane 6: Rat spleen lysate at 10 µg
Lane 7: C6 (Rat glial tumor cells) whole cell lysates at 10 µg
Lane 8: NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
![Western blot - Anti-GAPDH antibody [EPR16884] - Loading Control (ab181603), expandable thumbnail](https://content.abcam.com/products/images/gapdh-antibody-epr16884-loading-control-ab181603--western-blot-img106081.jpg "Western blot - Anti-GAPDH antibody [EPR16884] - Loading Control (ab181603)")
Western blot - Anti-GAPDH antibody [EPR16884] - Loading Control (ab181603)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-GAPDH antibody [EPR16884] - Loading Control (ab181603) at 1/10000 dilution
Lane 1: Human fetal brain lysate at 10 µg
Lane 2: Human fetal heart lysate at 10 µg
Lane 3: Human fetal kidney lysate at 10 µg
SecondaryAll lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody [EPR16884] - Loading Control (ab181603), expandable thumbnail](https://content.abcam.com/products/images/gapdh-antibody-epr16884-loading-control-ab181603--immunocytochemistry-immunofluorescence-img14153.jpg "Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody [EPR16884] - Loading Control (ab181603)")
Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody [EPR16884] - Loading Control (ab181603)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling GAPDH with ab181603 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/400 dilution (green). Cytoplasm and nuclear staining on HeLa cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows;
1. ab181603 at 1/250 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
2. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16884] - Loading Control (ab181603), expandable thumbnail](https://content.abcam.com/products/images/gapdh-antibody-epr16884-loading-control-ab181603--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img106692.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16884] - Loading Control (ab181603)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16884] - Loading Control (ab181603)
Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling GAPDH with ab181603 at 1/500 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasmic and nucleus staining on the tumor cells of transitional cell carcinoma of Human bladder is observed. Counter stained with Hematoxylin.
Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16884] - Loading Control (ab181603), expandable thumbnail](https://content.abcam.com/products/images/gapdh-antibody-epr16884-loading-control-ab181603--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img67418.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16884] - Loading Control (ab181603)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16884] - Loading Control (ab181603)
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling GAPDH with ab181603 at 1/500 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Nucleus and cytoplasmic staining on lymphocytes of mouse spleen is observed. Counter stained with Hematoxylin.
Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16884] - Loading Control (ab181603), expandable thumbnail](https://content.abcam.com/products/images/gapdh-antibody-epr16884-loading-control-ab181603--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109748.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16884] - Loading Control (ab181603)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16884] - Loading Control (ab181603)
Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling GAPDH with ab181603 at 1/500 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Nucleus and cytoplasmic staining on lymphocyte of rat spleen is observed. Counter stained with Hematoxylin.
Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-GAPDH antibody [EPR16884] - Loading Control (ab181603), expandable thumbnail](https://content.abcam.com/products/images/gapdh-antibody-epr16884-loading-control-ab181603--flow-cytometry-intracellular-img118155.jpg "Flow Cytometry (Intracellular) - Anti-GAPDH antibody [EPR16884] - Loading Control (ab181603)")
Flow Cytometry (Intracellular) - Anti-GAPDH antibody [EPR16884] - Loading Control (ab181603)
Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling GAPDH with ab181603 at 1/200 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16884] - Loading Control (ab181603), expandable thumbnail](https://content.abcam.com/products/images/gapdh-antibody-epr16884-loading-control-ab181603--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img106692.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16884] - Loading Control (ab181603)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16884] - Loading Control (ab181603), expandable thumbnail](https://content.abcam.com/products/images/gapdh-antibody-epr16884-loading-control-ab181603--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img67418.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16884] - Loading Control (ab181603)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16884] - Loading Control (ab181603), expandable thumbnail](https://content.abcam.com/products/images/gapdh-antibody-epr16884-loading-control-ab181603--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109748.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16884] - Loading Control (ab181603)")
![Flow Cytometry (Intracellular) - Anti-GAPDH antibody [EPR16884] - Loading Control (ab181603), expandable thumbnail](https://content.abcam.com/products/images/gapdh-antibody-epr16884-loading-control-ab181603--flow-cytometry-intracellular-img118155.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-GAPDH antibody [EPR16884] - Loading Control (ab181603)")
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