Anti-GAPDH antibody [FF26A] - Loading Control

• IP

Lab

Immunoprecipitation - Anti-GAPDH antibody [FF26A] - Loading Control (AB59164)

GADPH was immunoprecipitated from HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate with ab59164 at 14/7μg/ml. Western blot was performed from the immunoprecipitate using ab59164 at 1/1000 dilution. Anti-mouse IgG for IP (HRP) (ab131368) was used at 1/5000 dilution.
Lane 1 : HepG2 whole cell lysate 10μg (Input).
Lane 2 : ab59164 IP in HepG2 whole cell lysate.
Lane 3 : Mouse monoclonal IgG instead of ab59164 in HepG2 whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 5 secs.

All lanes:

Immunoprecipitation - Anti-GAPDH antibody [FF26A] - Loading Control (ab59164)

Predicted band size: 36 kDa

false

• IP

Lab

Immunoprecipitation - Anti-GAPDH antibody [FF26A] - Loading Control (AB59164)

GADPH was immunoprecipitated from MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate with ab59164 at 14.7μg/ml. Western blot was performed from the immunoprecipitate using ab59164 at 1/1000 dilution. Anti-mouse IgG for IP (HRP) (ab131368) was used at 1/5000 dilution.
Lane 1 : MDA-MB-231 whole cell lysate 10μg (Input).
Lane 2 : ab59164 IP in MDA-MB-231 whole cell lysate.
Lane 3 : Mouse monoclonal IgG instead of ab59164 in MDA-MB-231 whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 5 secs.

All lanes:

Immunoprecipitation - Anti-GAPDH antibody [FF26A] - Loading Control (ab59164)

Predicted band size: 36 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody [FF26A] - Loading Control (AB59164)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lanes 1-5 : 7 secs; Lanes 6-9 : 10 secs.

All lanes:

Western blot - Anti-GAPDH antibody [FF26A] - Loading Control (ab59164) at 1/2000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg

Lane 3:

Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 20 µg

Lane 4:

A549 (human lung carcinoma epithelial cell), whole cell lysate at 20 µg

Lane 5:

MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 6:

Human brain tissue lysate at 10 µg

Lane 7:

Human heart tissue lysate at 10 µg

Lane 8:

Human kidney tissue lysate at 10 µg

Lane 9:

Human spleen tissue lysate at 10 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 36 kDa

false