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Mouse Recombinant Monoclonal GAPDH antibody. Suitable for IP, WB and reacts with Human samples. Cited in 10 publications.

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Images

Immunoprecipitation - Anti-GAPDH antibody [FF26A] - Loading Control (AB59164), expandable thumbnail
  • Western blot - Anti-GAPDH antibody [FF26A] - Loading Control (AB59164), expandable thumbnail
  • Immunoprecipitation - Anti-GAPDH antibody [FF26A] - Loading Control (AB59164), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-P
Human
Tested
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
14.7 µg/mL
Notes

-

Tested
Tested

Species
Human
Dilution info
1/2000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Target data

Function

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively (PubMed:11724794, PubMed:3170585). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate (PubMed:11724794, PubMed:3170585). Modulates the organization and assembly of the cytoskeleton (By similarity). Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes (PubMed:23071094). Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (PubMed:23071094). Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (PubMed:23332158, PubMed:27387501). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis (By similarity). Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity).

Alternative names

GAPD, CDABP0047, OK/SW-cl.12, GAPDH, Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH

Recommended products

Mouse Recombinant Monoclonal GAPDH antibody. Suitable for IP, WB and reacts with Human samples. Cited in 10 publications.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
FF26A
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Glyceraldehyde-3-phosphate dehydrogenase commonly known as GAPDH is an enzyme involved in glycolysis. Its molecular weight (MW) is approximately 36 kDa. The protein is expressed ubiquitously in almost all tissues reflecting its essential role in energy production. GAPDH catalyzes the sixth step of glycolysis converting glyceraldehyde-3-phosphate into 13-bisphosphoglycerate. Due to its stable expression researchers often use GAPDH as a loading control in western blot experiments.

Biological function summary

GAPDH serves important metabolic functions beyond its enzymatic role in glycolysis. It functions as part of a multi-enzyme complex within the cytoplasm which facilitates efficient substrate channeling during glycolysis. Additionally GAPDH has non-glycolytic roles including involvement in nuclear processes like RNA export and DNA repair. Its ubiquitous presence across different cellular compartments indicates its multiple functions beyond metabolic pathways.

Pathways

GAPDH integrates into significant cellular functions like the glycolytic pathway and apoptotic pathways. In glycolysis GAPDH collaborates with enzymes like phosphoglycerate kinase forming a cohesive link in the energy conversion chain. Its participation in apoptotic pathways highlights GAPDH's involvement in cellular death processes interacting with proteins like Bcl-2 to influence apoptosis progression. These roles reinforce its presence in central metabolic and regulatory pathways.

Associated diseases and disorders

GAPDH has associations with neurodegenerative diseases and cancer. In neurodegenerative disorders such as Alzheimer's disease GAPDH’s altered enzymatic activity is frequently observed influencing cellular energy homeostasis. Moreover overexpression or aberrant regulation of GAPDH relates to cancer cell proliferation and metastasis implicating proteins like p53 in these pathways. The diverse functions and interactions of GAPDH emphasize its importance in both normal cellular function and disease states.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunoprecipitation - Anti-GAPDH antibody [FF26A] - Loading Control (ab59164), expandable thumbnail

    Immunoprecipitation - Anti-GAPDH antibody [FF26A] - Loading Control (ab59164)

    GADPH was immunoprecipitated from HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate with ab59164 at 14/7μg/ml. Western blot was performed from the immunoprecipitate using ab59164 at 1/1000 dilution. Anti-mouse IgG for IP (HRP) (Anti-mouse IgG for IP (HRP) ab131368) was used at 1/5000 dilution.
    Lane 1: HepG2 whole cell lysate 10μg (Input).
    Lane 2: ab59164 IP in HepG2 whole cell lysate.
    Lane 3: Mouse monoclonal IgG instead of ab59164 in HepG2 whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 5 secs.

    All lanes: Immunoprecipitation - Anti-GAPDH antibody [FF26A] - Loading Control (ab59164)

    Predicted band size: 36 kDa

  • Western blot - Anti-GAPDH antibody [FF26A] - Loading Control (ab59164), expandable thumbnail

    Western blot - Anti-GAPDH antibody [FF26A] - Loading Control (ab59164)

    GAPDH Western blot staining using mouse Anti-GAPDH antibody

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-5: 7 secs; Lanes 6-9: 10 secs.

    All lanes: Western blot - Anti-GAPDH antibody [FF26A] - Loading Control (ab59164) at 1/2000 dilution

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 2: HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 3: Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 20 µg

    Lane 4: A549 (human lung carcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 5: MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 6: Human brain tissue lysate at 10 µg

    Lane 7: Human heart tissue lysate at 10 µg

    Lane 8: Human kidney tissue lysate at 10 µg

    Lane 9: Human spleen tissue lysate at 10 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 36 kDa

  • Immunoprecipitation - Anti-GAPDH antibody [FF26A] - Loading Control (ab59164), expandable thumbnail

    Immunoprecipitation - Anti-GAPDH antibody [FF26A] - Loading Control (ab59164)

    GADPH was immunoprecipitated from MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate with ab59164 at 14.7μg/ml. Western blot was performed from the immunoprecipitate using ab59164 at 1/1000 dilution. Anti-mouse IgG for IP (HRP) (Anti-mouse IgG for IP (HRP) ab131368) was used at 1/5000 dilution.
    Lane 1: MDA-MB-231 whole cell lysate 10μg (Input).
    Lane 2: ab59164 IP in MDA-MB-231 whole cell lysate.
    Lane 3: Mouse monoclonal IgG instead of ab59164 in MDA-MB-231 whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 5 secs.

    All lanes: Immunoprecipitation - Anti-GAPDH antibody [FF26A] - Loading Control (ab59164)

    Predicted band size: 36 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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