AB125247

Anti-GAPDH antibody [GA1R] - Loading Control

Name: Anti-GAPDH antibody [GA1R] - Loading Control (ab125247) | Abcam
Brand: Abcam Limited
SKU: AB125247
Price: 400 USD
Availability: InStock
Rating: 5 (7 reviews)

Lab Essentials
What is this?

(7 Reviews)

(177 Publications)

Anti-GAPDH antibody [GA1R] - Loading Control (ab125247) is a mouse monoclonal antibody detecting GAPDH in Western Blot. Suitable for Chicken, Escherichia coli, Hamster, Human, Mouse, Rabbit, Rat, S.cerevisiae, Spodoptera frugiperda (SF9 cells).

- Over 130 publications

View Alternative Names

GAPD, CDABP0047, OK/SW-cl.12, GAPDH, Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH

2 Images

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Western blot - Anti-GAPDH antibody [GA1R] - Loading Control (AB125247)

All lanes:

Western blot - Anti-GAPDH antibody [GA1R] - Loading Control (ab125247) at 1/2000 dilution

Lane 1:

BL21 bacterial lysate at 5 µg

Lane 2:

Sf9 insect lysate at 5 µg

Lane 3:

Saccharomyces cerevisiae lysate at 5 µg

Predicted band size: 36 kDa

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Western blot - Anti-GAPDH antibody [GA1R] - Loading Control (AB125247)

All lanes:

Western blot - Anti-GAPDH antibody [GA1R] - Loading Control (ab125247) at 1/2000 dilution

Lane 1:

Human tissue lysate at 5 µg

Lane 2:

Mouse tissue lysate at 5 µg

Lane 3:

Rat tissue lysate at 5 µg

Lane 4:

Rabbit tissue lysate at 5 µg

Lane 5:

Chicken tissue lysate at 5 µg

Lane 6:

Hamster tissue lysate at 5 µg

Predicted band size: 36 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

GA1R

Isotype

IgG1

Carrier free

Reacts with

Mouse, Rat, Rabbit, Chicken, Hamster, Human, Saccharomyces cerevisiae, Escherichia coli, Fall armyworm

Applications

applications

Immunogen

Recombinant Full Length Protein corresponding to Rabbit GAPDH.

P46406

Specificity

ab125247 recognizes native and denatured forms of GAPDH.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "with ECL." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "with ECL." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "with ECL." }, "Chicken": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "with ECL." }, "Escherichia coli": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "with ECL." }, "Fall armyworm": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Hamster": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "with ECL." }, "Rabbit": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "with ECL." }, "Saccharomyces cerevisiae": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "with ECL." } } }

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Product details

What is this antibody validated in?
Anti-GAPDH antibody [GA1R] - Loading Control (ab125247) is a mouse monoclonal antibody and is validated for use in Western Blot (WB) in Chicken, Escherichia coli, Hamster, Human, Mouse, Rabbit, Rat, Saccharomyces cerevisiae, Spodoptera frugiperda (SF9 cells) samples.

What is the molecular weight of GAPDH?
Anti-GAPDH [GA1R] - Loading Control (ab125247) is often used as a loading control in Western blot. It specifically detects a band for GAPDH (UniProt: P04406) at a molecular weight of 36kDa.

Trusted by the scientific community
Anti-GAPDH [GA1R] - Loading Control (ab125247) was first used in a scientific publication in 2012 and has been cited over 130 times in peer-reviewed journals.

Reviewed by scientists
Anti-GAPDH [GA1R] - Loading Control (ab125247) has over 5 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Protein A affinity chromatography from TCS

Storage buffer

pH: 7.2 Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glyceraldehyde-3-phosphate dehydrogenase commonly known as GAPDH is an enzyme involved in glycolysis. Its molecular weight (MW) is approximately 36 kDa. The protein is expressed ubiquitously in almost all tissues reflecting its essential role in energy production. GAPDH catalyzes the sixth step of glycolysis converting glyceraldehyde-3-phosphate into 13-bisphosphoglycerate. Due to its stable expression researchers often use GAPDH as a loading control in western blot experiments.

Biological function summary

GAPDH serves important metabolic functions beyond its enzymatic role in glycolysis. It functions as part of a multi-enzyme complex within the cytoplasm which facilitates efficient substrate channeling during glycolysis. Additionally GAPDH has non-glycolytic roles including involvement in nuclear processes like RNA export and DNA repair. Its ubiquitous presence across different cellular compartments indicates its multiple functions beyond metabolic pathways.

Pathways

GAPDH integrates into significant cellular functions like the glycolytic pathway and apoptotic pathways. In glycolysis GAPDH collaborates with enzymes like phosphoglycerate kinase forming a cohesive link in the energy conversion chain. Its participation in apoptotic pathways highlights GAPDH's involvement in cellular death processes interacting with proteins like Bcl-2 to influence apoptosis progression. These roles reinforce its presence in central metabolic and regulatory pathways.

GAPDH has associations with neurodegenerative diseases and cancer. In neurodegenerative disorders such as Alzheimer's disease GAPDH’s altered enzymatic activity is frequently observed influencing cellular energy homeostasis. Moreover overexpression or aberrant regulation of GAPDH relates to cancer cell proliferation and metastasis implicating proteins like p53 in these pathways. The diverse functions and interactions of GAPDH emphasize its importance in both normal cellular function and disease states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively (PubMed : 11724794, PubMed : 3170585). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate (PubMed : 11724794, PubMed : 3170585). Modulates the organization and assembly of the cytoskeleton (By similarity). Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes (PubMed : 23071094). Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (PubMed : 23071094). Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (PubMed : 23332158, PubMed : 27387501). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis (By similarity). Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity).

See full target information GAPDH

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Publications (177)

Recent publications for all applications. Explore the full list and refine your search

Journal of thoracic disease 17:4249-4263 PubMed40688308

2025

regulation of cardiac fibroblast proliferation via the TGF-β/Smad pathway in an model of atrial fibrillation.

Applications

Unspecified application

Species

Unspecified reactive species

Yimeng Zhou,Lianzhi Zhang,Wenjia Zhang,Qunlin Gong,Zhong Zhang,Nan Xu,Ashley R Wilson-Smith,Nannan Chen

Journal of thoracic disease 17:4238-4248 PubMed40688289

2025

Effect of pirfenidone on pulmonary fibrosis in acute lung injury via the regulation of the miR-34a-5p/TGF-β1/SMAD pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chengyue Yu,Yonghuai Li,Youhui Tu,Mengyuan Dai,Liming Fei

Nucleic acids research 53: PubMed40613706

2025

The Epstein-Barr virus EBNA1 protein binds to G-quadruplexes in its own mRNA hence controlling its expression and immune evasion of the virus.

Applications

Unspecified application

Species

Unspecified reactive species

Van-Trang Dinh,Nadège Loaëc,Alicia Quillévéré,Marc Keruzoré,Aline Peynet,Ronan Le Sénéchal,Marie-Paule Teulade-Fichou,Laurent Corcos,Rodrigo Prado Martins,Anton Granzhan,Marc Blondel

Life science alliance 8: PubMed40441895

2025

Tbx1 plays a critical role in focal adhesion dynamics through paxillin regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Olimpia Iacolare,Rosa Ferrentino,Alessandra Altomonte,Christopher E Turner,Antonio Baldini,Daniela Alfano

Biomaterials research 29:0201 PubMed40330275

2025

Impact of Culture Duration on the Properties and Functionality of Yeast-Derived Extracellular Vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Gyeongchan Jeon,Yang-Hoon Kim,Jiho Min

Nature communications 16:4055 PubMed40307206

2025

Allelic effects on KLHL17 expression underlie a pancreatic cancer genome-wide association signal at chr1p36.33.

Applications

Unspecified application

Species

Unspecified reactive species

Katelyn E Connelly,Katherine Hullin,Ehssan Abdolalizadeh,Jun Zhong,Daina Eiser,Aidan O'Brien,Irene Collins,Sudipto Das,Gerard Duncan,Stephen J Chanock,Rachael Z Stolzenberg-Solomon,Alison P Klein,Brian M Wolpin,Jason W Hoskins,Thorkell Andresson,Jill P Smith,Laufey T Amundadottir

Nature communications 16:3269 PubMed40188181

2025

scRNA-seq uncovers the transcriptional dynamics of Encephalitozoon intestinalis parasites in human macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Pattana Jaroenlak,Kacie L McCarty,Bo Xia,Cherry Lam,Erin E Zwack,Nadia L Almasri,Joseph Sudar,Maelle Aubry,Itai Yanai,Gira Bhabha,Damian C Ekiert

ESC heart failure 12:2256-2266 PubMed40025327

2025

Calpain inhibition in a transgenic model of calpastatin overexpression facilitates reversal of myocardial hypertrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Gregor Sachse,Johanna Tennigkeit,Nikolaos Pagonas,Philipp Hillmeister,Ivo Buschmann,Martin Czolbe,Peter Nordbeck,Joachim Schmitt,Daniel Patschan,Oliver Ritter

Annals of surgical oncology 32:3839-3850 PubMed39953349

2025

Clinical Significance of Coiled-Coil Domain-Containing Protein 25 Expression in Esophageal Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Takafumi Suzuki,Hironori Tsujimoto,Takanori Watanabe,Yusuke Ishibashi,Seiichiro Fujishima,Yujiro Itazaki,Risa Kariya,Naoyuki Uehata,Hanae Shinada,Satsuki Mochizuki,Yoji Kishi,Kimiya Sato,Hideki Ueno

The Journal of general virology 106: PubMed39928567

2025

The EMCV protein 2B* is required for efficient cell lysis via both caspase-3-dependent and -independent pathways during infection.

Applications

Unspecified application

Species

Unspecified reactive species

Samantha K Nguyen,Edward Long,James R Edgar,Andrew E Firth,Hazel Stewart

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GAPDH antibody [GA1R] - Loading Control

Western blot - Anti-GAPDH antibody [GA1R] - Loading Control (AB125247)

Western blot - Anti-GAPDH antibody [GA1R] - Loading Control (AB125247)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (177)

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