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Mouse Monoclonal GAPDH antibody. Suitable for WB and reacts with Mouse, Rat, Hamster, Saccharomyces cerevisiae, Escherichia coli, Chicken, Human, Rabbit samples. Cited in 132 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Rabbit GAPDH.


Images

Western blot - Anti-GAPDH antibody [GA1R] - Loading Control (AB125247), expandable thumbnail
  • Western blot - Anti-GAPDH antibody [GA1R] - Loading Control (AB125247), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Rabbit GAPDH. Database link P46406

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Tested
Rat
Tested
Chicken
Tested
Escherichia coli
Tested
Fall armyworm
Predicted
Hamster
Tested
Rabbit
Tested
Saccharomyces cerevisiae
Tested

Tested
Tested

Species

Mouse

Dilution info

1/1000.00000 - 1/10000.00000

Notes

with ECL.

Species

Rat

Dilution info

1/1000.00000 - 1/10000.00000

Notes

with ECL.

Species

Hamster

Dilution info

1/1000.00000 - 1/10000.00000

Notes

with ECL.

Species

Saccharomyces cerevisiae

Dilution info

1/1000.00000 - 1/10000.00000

Notes

with ECL.

Species

Escherichia coli

Dilution info

1/1000.00000 - 1/10000.00000

Notes

with ECL.

Species

Chicken

Dilution info

1/1000.00000 - 1/10000.00000

Notes

with ECL.

Species

Human

Dilution info

1/1000.00000 - 1/10000.00000

Notes

with ECL.

Species

Rabbit

Dilution info

1/1000.00000 - 1/10000.00000

Notes

with ECL.

Predicted
Predicted

Species

Fall armyworm

Dilution info

-

Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively (PubMed:11724794, PubMed:3170585). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate (PubMed:11724794, PubMed:3170585). Modulates the organization and assembly of the cytoskeleton (By similarity). Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes (PubMed:23071094). Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (PubMed:23071094). Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (PubMed:23332158, PubMed:27387501). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis (By similarity). Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity).

Alternative names

Recommended products

Mouse Monoclonal GAPDH antibody. Suitable for WB and reacts with Mouse, Rat, Hamster, Saccharomyces cerevisiae, Escherichia coli, Chicken, Human, Rabbit samples. Cited in 132 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Rabbit GAPDH.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Full Length Protein corresponding to Rabbit GAPDH. Database link P46406
Clone number

GA1R

Purification technique

Affinity purification Protein A

Specificity

ab125247 recognizes native and denatured forms of GAPDH.

Concentration
Loading...
Purification notes

Protein A affinity chromatography from TCS

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Store at 4°C (add 0.05% NaN3) for several days to weeks.

This product was changed from ascites to tissue culture supernatant on 5th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Glyceraldehyde-3-phosphate dehydrogenase commonly known as GAPDH is an enzyme involved in glycolysis. Its molecular weight (MW) is approximately 36 kDa. The protein is expressed ubiquitously in almost all tissues reflecting its essential role in energy production. GAPDH catalyzes the sixth step of glycolysis converting glyceraldehyde-3-phosphate into 13-bisphosphoglycerate. Due to its stable expression researchers often use GAPDH as a loading control in western blot experiments.

Biological function summary

GAPDH serves important metabolic functions beyond its enzymatic role in glycolysis. It functions as part of a multi-enzyme complex within the cytoplasm which facilitates efficient substrate channeling during glycolysis. Additionally GAPDH has non-glycolytic roles including involvement in nuclear processes like RNA export and DNA repair. Its ubiquitous presence across different cellular compartments indicates its multiple functions beyond metabolic pathways.

Pathways

GAPDH integrates into significant cellular functions like the glycolytic pathway and apoptotic pathways. In glycolysis GAPDH collaborates with enzymes like phosphoglycerate kinase forming a cohesive link in the energy conversion chain. Its participation in apoptotic pathways highlights GAPDH's involvement in cellular death processes interacting with proteins like Bcl-2 to influence apoptosis progression. These roles reinforce its presence in central metabolic and regulatory pathways.

Associated diseases and disorders

GAPDH has associations with neurodegenerative diseases and cancer. In neurodegenerative disorders such as Alzheimer's disease GAPDH’s altered enzymatic activity is frequently observed influencing cellular energy homeostasis. Moreover overexpression or aberrant regulation of GAPDH relates to cancer cell proliferation and metastasis implicating proteins like p53 in these pathways. The diverse functions and interactions of GAPDH emphasize its importance in both normal cellular function and disease states.

Product promise

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2 product images

  • Western blot - Anti-GAPDH antibody [GA1R] - Loading Control (ab125247), expandable thumbnail

    Western blot - Anti-GAPDH antibody [GA1R] - Loading Control (ab125247)

    All lanes: Western blot - Anti-GAPDH antibody [GA1R] - Loading Control (ab125247) at 1/2000 dilution

    Lane 1: Human tissue lysate at 5 µg

    Lane 2: Mouse tissue lysate at 5 µg

    Lane 3: Rat tissue lysate at 5 µg

    Lane 4: Rabbit tissue lysate at 5 µg

    Lane 5: Chicken tissue lysate at 5 µg

    Lane 6: Hamster tissue lysate at 5 µg

    Developed using the ECL technique.

    Predicted band size: 36 kDa

  • Western blot - Anti-GAPDH antibody [GA1R] - Loading Control (ab125247), expandable thumbnail

    Western blot - Anti-GAPDH antibody [GA1R] - Loading Control (ab125247)

    All lanes: Western blot - Anti-GAPDH antibody [GA1R] - Loading Control (ab125247) at 1/2000 dilution

    Lane 1: BL21 bacterial lysate at 5 µg

    Lane 2: Sf9 insect lysate at 5 µg

    Lane 3: Saccharomyces cerevisiae lysate at 5 µg

    Developed using the ECL technique.

    Predicted band size: 36 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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