Goat Polyclonal GAPDH antibody. Suitable for WB, IHC-Fr and reacts with Human, Mouse samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human GAPDH aa 150-250.
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- Proceedings of the National Academy of Sciences of the United States of America 120:e22168871202023Fragile X mental retardation protein coordinates neuron-to-glia communication for clearance of developmentally transient brain neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChunzhu Song,Kendal BroadiePubMed 36920921
- Viruses 14:2022Investigation of the Association between the Energy Metabolism of the Insect Vector and Rice Stripe Virus (RSV).Applications:Unspecified applicationReactive species:Unspecified reactive speciesLu Zhang,Xinyi Li,Yan Chen,Lin Kang,Jiao Zhang,Yao Li,Fang LiuPubMed 36298853
- Reproduction in domestic animals = Zuchthygiene 57 Suppl 5:78-812022Changes in aquaporins mRNA expression and liquid storage at 17°C: A potential biomarker of boar sperm quality?Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarina Castany Quintana,Jaume Gardela,Mateo Ruiz-Conca,Manel López-Béjar,Cristina A Martinez,Heriberto Rodríguez-Martinez,Manuel Álvarez-RodríguezPubMed 35467055
- Frontiers in molecular neuroscience 14:7478552021Molecular Changes in Prader-Willi Syndrome Neurons Reveals Clues About Increased Autism Susceptibility.Applications:Unspecified applicationReactive species:Unspecified reactive speciesA Kaitlyn Victor,Martin Donaldson,Daniel Johnson,Winston Miller,Lawrence T ReiterPubMed 34776864
- eNeuro 8:2021Beneficial Effects of Transplanted Human Bone Marrow Endothelial Progenitors on Functional and Cellular Components of Blood-Spinal Cord Barrier in ALS Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSvitlana Garbuzova-Davis,Kayla J Boccio,Alexander Llauget,Robert Shell,Surafuale Hailu,Hilmi Mustafa,Jared Ehrhart,Paul R Sanberg,Stanley H Appel,Cesario V BorlonganPubMed 34479980
- PLoS pathogens 16:e10087102020The α-tubulin of Laodelphax striatellus mediates the passage of rice stripe virus (RSV) and enhances horizontal transmission.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYao Li et. al.PubMed 32817722
- Neurobiology of disease 141:1048792020Transcriptomic and proteomic profiling of glial versus neuronal Dube3a overexpression reveals common molecular changes in gliopathic epilepsies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKevin A Hope et. al.PubMed 32344153
- Cell biology international 44:1447-14572020YY1-induced upregulation of FOXP4-AS1 and FOXP4 promote the proliferation of esophageal squamous cell carcinoma cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYonghui Li,Tingting Li,Yongbin Yang,Wenli Kang,Shaoyong Dong,Shujie ChengPubMed 32159250
- Bioscience reports 40:2020Differential regulation of sFlt-1 splicing by U2AF65 and JMJD6 in placental-derived and endothelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdrian C Eddy,Heather Chapman,David T Brown,Eric M GeorgePubMed 32039444
- Thoracic cancer 9:1262-12702018MiR-873 inhibition enhances gefitinib resistance in non-small cell lung cancer cells by targeting glioma-associated oncogene homolog 1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShidai Jin et. al.PubMed 30126075
Key facts
- Isotype
IgG
- Host species
Goat
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human GAPDH aa 150-250. The exact immunogen used to generate this antibody is proprietary information. Database link P04406
Consider this alternative
Reactivity data
Select an application| WB | IHC-Fr | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Predicted | Predicted |
| Caenorhabditis elegans | Predicted | Predicted |
| Chicken | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Drosophila melanogaster | Predicted | Predicted |
| Guinea pig | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
| Sheep | Predicted | Predicted |
| Turkey | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted |
| Xenopus tropicalis | Predicted | Predicted |
| Zebrafish | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/2500 | Notes - |
Species Mouse | Dilution info 1/500 - 1/2500 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Sheep, Rabbit, Chicken, Guinea pig, Cow, Turkey, Pig, Xenopus laevis, Caenorhabditis elegans, Drosophila melanogaster, Zebrafish, Orangutan, Xenopus tropicalis | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/2000 | Notes Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Sheep, Rabbit, Chicken, Guinea pig, Cow, Turkey, Pig, Xenopus laevis, Caenorhabditis elegans, Drosophila melanogaster, Zebrafish, Orangutan, Xenopus tropicalis | Dilution info - | Notes - |
Associated Products
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Target data
Function
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively (PubMed:11724794, PubMed:3170585). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate (PubMed:11724794, PubMed:3170585). Modulates the organization and assembly of the cytoskeleton (By similarity). Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes (PubMed:23071094). Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (PubMed:23071094). Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (PubMed:23332158, PubMed:27387501). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis (By similarity). Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity).
Alternative names
GAPD, CDABP0047, OK/SW-cl.12, GAPDH, Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH
Recommended products
Goat Polyclonal GAPDH antibody. Suitable for WB, IHC-Fr and reacts with Human, Mouse samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human GAPDH aa 150-250.
Key facts
- Isotype
IgG
- Host species
Goat
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human GAPDH aa 150-250. The exact immunogen used to generate this antibody is proprietary information. Database link P04406
- Purification technique
Affinity purification Immunogen
- Concentration
- Purification notes
ab157156 was affinity purified using an epitope specific to GAPDH immobilized on solid support.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Glyceraldehyde-3-phosphate dehydrogenase commonly known as GAPDH is an enzyme involved in glycolysis. Its molecular weight (MW) is approximately 36 kDa. The protein is expressed ubiquitously in almost all tissues reflecting its essential role in energy production. GAPDH catalyzes the sixth step of glycolysis converting glyceraldehyde-3-phosphate into 13-bisphosphoglycerate. Due to its stable expression researchers often use GAPDH as a loading control in western blot experiments.
- Biological function summary
GAPDH serves important metabolic functions beyond its enzymatic role in glycolysis. It functions as part of a multi-enzyme complex within the cytoplasm which facilitates efficient substrate channeling during glycolysis. Additionally GAPDH has non-glycolytic roles including involvement in nuclear processes like RNA export and DNA repair. Its ubiquitous presence across different cellular compartments indicates its multiple functions beyond metabolic pathways.
- Pathways
GAPDH integrates into significant cellular functions like the glycolytic pathway and apoptotic pathways. In glycolysis GAPDH collaborates with enzymes like phosphoglycerate kinase forming a cohesive link in the energy conversion chain. Its participation in apoptotic pathways highlights GAPDH's involvement in cellular death processes interacting with proteins like Bcl-2 to influence apoptosis progression. These roles reinforce its presence in central metabolic and regulatory pathways.
- Associated diseases and disorders
GAPDH has associations with neurodegenerative diseases and cancer. In neurodegenerative disorders such as Alzheimer's disease GAPDH’s altered enzymatic activity is frequently observed influencing cellular energy homeostasis. Moreover overexpression or aberrant regulation of GAPDH relates to cancer cell proliferation and metastasis implicating proteins like p53 in these pathways. The diverse functions and interactions of GAPDH emphasize its importance in both normal cellular function and disease states.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Frozen sections) - Anti-GAPDH antibody - Loading Control (ab157156)
Immunohistochemical analysis of frozen sections of human colon carcinoma tissue labelling GAPDH with ab157156 at 1/1000 (1μg/ml). Detection: DAB.
Western blot - Anti-GAPDH antibody - Loading Control (ab157156)
All lanes: Western blot - Anti-GAPDH antibody - Loading Control (ab157156) at 1 µg/mL
Lane 1: 293T whole cell lysate at 50 µg
Lane 2: 293T whole cell lysate at 15 µg
Lane 3: HeLa whole cell lysate at 50 µg
Lane 4: Jurkat whole cell lysate at 50 µg
Lane 5: NIH 3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 36 kDa
Exposure time: 30s
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