AB210113

Anti-GAPDH antibody - Loading Control

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(7 Publications)

Rabbit Polyclonal GAPDH antibody. Suitable for ICC/IF, IHC-P, WB and reacts with Human, Zebrafish, Mouse, Rat, Dog, Cat, Escherichia coli, Drosophila melanogaster samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Zebrafish gapdh aa 100-200.

View Alternative Names

Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH, gapdh

10 Images

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB210113)

Immunofluorescence analysis of HeLa cells fixed in ice-cold MeOH for 5 min labelling Gapdh protein at cytoplasm using ab210113 at a 1/6000 dilution (Green).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody - Loading Control (AB210113)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue stained for Gapdh protein at cytoplasm and nucleus with ab210113 at a 1/1000 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min.

Supplier Data

Western blot - Anti-GAPDH antibody - Loading Control (AB210113)

Samples were separated by 10 % SDS.

Lane 1:

A431 whole cell extracts at 30 µg

Lane 2:

HeLa whole cell extracts at 30 µg

Lane 3:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 36 kDa

false

Supplier Data

Western blot - Anti-GAPDH antibody - Loading Control (AB210113)

Samples were separated by 10 % SDS.

Lane 1:

Neuro2A whole cell lysates at 30 µg

Lane 2:

C8D30 whole cell lysates at 30 µg

Lane 3:

NIH-3T3 whole cell lysates at 30 µg

Lane 4:

Raw264.7 whole cell lysates at 30 µg

Lane 5:

C2C12 whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 36 kDa

false

Supplier Data

Western blot - Anti-GAPDH antibody - Loading Control (AB210113)

Samples were separated by 10 % SDS.

All lanes:

Pc12 Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 36 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody - Loading Control (AB210113)

Immunohistochemical analysis of paraffin-embedded Zebrafish tissue labeling GAPDH with ab210113 at 1/300 dilution.

Supplier Data

Western blot - Anti-GAPDH antibody - Loading Control (AB210113)

Samples were separated by 10 % SDS.

All lanes:

Whole zebrafish extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 36 kDa

false

Supplier Data

Western blot - Anti-GAPDH antibody - Loading Control (AB210113)

Samples were separated by 10 % SDS.

All lanes:

Drosophila extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 36 kDa

false

Supplier Data

Western blot - Anti-GAPDH antibody - Loading Control (AB210113)

Samples were separated by 10 % SDS.

All lanes:

E. coli M15 Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 36 kDa

false

Supplier Data

Western blot - Anti-GAPDH antibody - Loading Control (AB210113)

Samples were separated by 10 % SDS.

Lane 1:

MDCK whole cell extracts at 30 µg

Lane 2:

PG-4 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 36 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Cat, Dog, Human, Zebrafish, Escherichia coli, Drosophila melanogaster

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Zebrafish gapdh aa 100-200. The exact immunogen used to generate this antibody is proprietary information.

Q5XJ10

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Cat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Chicken": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Drosophila melanogaster": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Escherichia coli": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Guinea pig": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glyceraldehyde-3-phosphate dehydrogenase commonly known as GAPDH is an enzyme involved in glycolysis. Its molecular weight (MW) is approximately 36 kDa. The protein is expressed ubiquitously in almost all tissues reflecting its essential role in energy production. GAPDH catalyzes the sixth step of glycolysis converting glyceraldehyde-3-phosphate into 13-bisphosphoglycerate. Due to its stable expression researchers often use GAPDH as a loading control in western blot experiments.

Biological function summary

GAPDH serves important metabolic functions beyond its enzymatic role in glycolysis. It functions as part of a multi-enzyme complex within the cytoplasm which facilitates efficient substrate channeling during glycolysis. Additionally GAPDH has non-glycolytic roles including involvement in nuclear processes like RNA export and DNA repair. Its ubiquitous presence across different cellular compartments indicates its multiple functions beyond metabolic pathways.

Pathways

GAPDH integrates into significant cellular functions like the glycolytic pathway and apoptotic pathways. In glycolysis GAPDH collaborates with enzymes like phosphoglycerate kinase forming a cohesive link in the energy conversion chain. Its participation in apoptotic pathways highlights GAPDH's involvement in cellular death processes interacting with proteins like Bcl-2 to influence apoptosis progression. These roles reinforce its presence in central metabolic and regulatory pathways.

GAPDH has associations with neurodegenerative diseases and cancer. In neurodegenerative disorders such as Alzheimer's disease GAPDH’s altered enzymatic activity is frequently observed influencing cellular energy homeostasis. Moreover overexpression or aberrant regulation of GAPDH relates to cancer cell proliferation and metastasis implicating proteins like p53 in these pathways. The diverse functions and interactions of GAPDH emphasize its importance in both normal cellular function and disease states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the conversion of D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate in glycolysis and the reverse reaction in gluconeogenesis. Also shows nitrosylase activity, thereby playing a role in nuclear functions (By similarity). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity).

See full target information gapdh

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in molecular neuroscience 18:1563086 PubMed40496801

2025

Exposure to a nanoplastic-enriched diet for fourteen days increases microglial immunoreactivity in the zebrafish telencephalon.

Applications

Unspecified application

Species

Unspecified reactive species

Robert A Mans,Hannah Kelehear,Sarah Rotschafer,Clare Ganas,Brendan Uche-Moon,Gabrielle Call,Callie C Mauersberg,Justin Toller,Andrew Diamanduros

International journal of molecular sciences 25: PubMed39684386

2024

Knockdown Induces Obesity and AHO Features in Early Zebrafish Larvae.

Applications

Unspecified application

Species

Unspecified reactive species

Alaa Abbas,Ayat S Hammad,Zain Z Zakaria,Maha Al-Asmakh,Khalid Hussain,Mashael Al-Shafai

Biomedicines 12: PubMed39595152

2024

Brain Injury and Neurodegeneration: Molecular, Functional, and Translational Approach 2.0.

Applications

Unspecified application

Species

Unspecified reactive species

Pankaj Ahluwalia,Pankaj Gaur,Meenakshi Ahluwalia,Kumar Vaibhav

Nature communications 15:3684 PubMed38693181

2024

Development of Aptamer-DNAzyme based metal-nucleic acid frameworks for gastric cancer therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaqi Yan,Rajendra Bhadane,Meixin Ran,Xiaodong Ma,Yuanqiang Li,Dongdong Zheng,Outi M H Salo-Ahen,Hongbo Zhang

International journal of molecular sciences 24: PubMed37298126

2023

Characterization of Macroglia Response during Tissue Repair in a Laser-Induced Model of Retinal Degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Jahnke,Souska Zandi,Ahmed Elhelbawi,Federica Maria Conedera,Volker Enzmann

Biomedicines 10: PubMed35052712

2021

Prophylactic Activation of Shh Signaling Attenuates TBI-Induced Seizures in Zebrafish by Modulating Glutamate Excitotoxicity through Eaat2a.

Applications

Unspecified application

Species

Unspecified reactive species

James Hentig,Leah J Campbell,Kaylee Cloghessy,Mijoon Lee,William Boggess,David R Hyde

The Journal of neuroscience : the official journal 40:3631-3645 PubMed32245827

2020

A Model to Study NMDA Receptors in Early Nervous System Development.

Applications

Unspecified application

Species

Unspecified reactive species

Josiah D Zoodsma,Kelvin Chan,Ashwin A Bhandiwad,David R Golann,Guangmei Liu,Shoaib A Syed,Amalia J Napoli,Harold A Burgess,Howard I Sirotkin,Lonnie P Wollmuth

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GAPDH antibody - Loading Control

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB210113)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody - Loading Control (AB210113)

Western blot - Anti-GAPDH antibody - Loading Control (AB210113)

Western blot - Anti-GAPDH antibody - Loading Control (AB210113)

Western blot - Anti-GAPDH antibody - Loading Control (AB210113)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody - Loading Control (AB210113)

Western blot - Anti-GAPDH antibody - Loading Control (AB210113)

Western blot - Anti-GAPDH antibody - Loading Control (AB210113)

Western blot - Anti-GAPDH antibody - Loading Control (AB210113)

Western blot - Anti-GAPDH antibody - Loading Control (AB210113)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise