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Rabbit Polyclonal GAPDH antibody. Suitable for IHC-P, WB and reacts with Zebrafish samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Zebrafish gapdh aa 100-200.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody - Loading Control (AB210113), expandable thumbnail
  • Western blot - Anti-GAPDH antibody - Loading Control (AB210113), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Zebrafish gapdh aa 100-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q5XJ10

Consider this alternative

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Predicted
Predicted
Mouse
Predicted
Predicted
Rat
Predicted
Predicted
Cat
Predicted
Predicted
Chicken
Predicted
Predicted
Dog
Predicted
Predicted
Guinea pig
Predicted
Predicted
Pig
Predicted
Predicted
Rabbit
Predicted
Predicted
Rhesus monkey
Predicted
Predicted
Xenopus laevis
Predicted
Predicted
Xenopus tropicalis
Predicted
Predicted
Zebrafish
Tested
Tested

Tested
Tested

Species

Zebrafish

Dilution info

1/100 - 1/1000

Notes

suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Predicted
Predicted

Species

Mouse, Rat, Rabbit, Chicken, Guinea pig, Cat, Dog, Human, Pig, Xenopus laevis, Rhesus monkey, Xenopus tropicalis

Dilution info

-

Notes

-

Tested
Tested

Species

Zebrafish

Dilution info

1/500 - 1/3000

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Rabbit, Chicken, Guinea pig, Cat, Dog, Human, Pig, Xenopus laevis, Rhesus monkey, Xenopus tropicalis

Dilution info

-

Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate (By similarity). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity).

Alternative names

Recommended products

Rabbit Polyclonal GAPDH antibody. Suitable for IHC-P, WB and reacts with Zebrafish samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Zebrafish gapdh aa 100-200.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Zebrafish gapdh aa 100-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q5XJ10
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Store in the dark

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Glyceraldehyde-3-phosphate dehydrogenase commonly known as GAPDH is an enzyme involved in glycolysis. Its molecular weight (MW) is approximately 36 kDa. The protein is expressed ubiquitously in almost all tissues reflecting its essential role in energy production. GAPDH catalyzes the sixth step of glycolysis converting glyceraldehyde-3-phosphate into 13-bisphosphoglycerate. Due to its stable expression researchers often use GAPDH as a loading control in western blot experiments.

Biological function summary

GAPDH serves important metabolic functions beyond its enzymatic role in glycolysis. It functions as part of a multi-enzyme complex within the cytoplasm which facilitates efficient substrate channeling during glycolysis. Additionally GAPDH has non-glycolytic roles including involvement in nuclear processes like RNA export and DNA repair. Its ubiquitous presence across different cellular compartments indicates its multiple functions beyond metabolic pathways.

Pathways

GAPDH integrates into significant cellular functions like the glycolytic pathway and apoptotic pathways. In glycolysis GAPDH collaborates with enzymes like phosphoglycerate kinase forming a cohesive link in the energy conversion chain. Its participation in apoptotic pathways highlights GAPDH's involvement in cellular death processes interacting with proteins like Bcl-2 to influence apoptosis progression. These roles reinforce its presence in central metabolic and regulatory pathways.

Associated diseases and disorders

GAPDH has associations with neurodegenerative diseases and cancer. In neurodegenerative disorders such as Alzheimer's disease GAPDH’s altered enzymatic activity is frequently observed influencing cellular energy homeostasis. Moreover overexpression or aberrant regulation of GAPDH relates to cancer cell proliferation and metastasis implicating proteins like p53 in these pathways. The diverse functions and interactions of GAPDH emphasize its importance in both normal cellular function and disease states.

Product promise

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2 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody - Loading Control (ab210113), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody - Loading Control (ab210113)

    Immunohistochemical analysis of paraffin-embedded Zebrafish tissue labeling GAPDH with ab210113 at 1/300 dilution.

  • Western blot - Anti-GAPDH antibody - Loading Control (ab210113), expandable thumbnail

    Western blot - Anti-GAPDH antibody - Loading Control (ab210113)

    10% SDS PAGE

    All lanes: Western blot - Anti-GAPDH antibody - Loading Control (ab210113) at 1/1000 dilution

    All lanes: Adult Zebrafish whole cell lysate at 10 µg

    Predicted band size: 36 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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