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Rabbit Polyclonal GAPDH antibody. Suitable for WB and reacts with Mouse, Rat, African green monkey, Human, Dog samples. Cited in 114 publications. Immunogen corresponding to Synthetic Peptide within Human GAPDH aa 100-150.


Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human GAPDH aa 100-150. The exact immunogen used to generate this antibody is proprietary information. Database link P04406

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Tested
Rat
Tested
African green monkey
Tested
Cat
Predicted
Chicken
Predicted
Cow
Predicted
Dog
Tested
Duck
Predicted
Guinea pig
Predicted
Horse
Predicted
Pig
Predicted
Rabbit
Predicted
Sheep
Predicted
Turkey
Predicted

Tested
Tested

Species

Mouse

Dilution info

1/1000 - 1/5000

Notes

-

Species

Rat

Dilution info

1/1000 - 1/5000

Notes

-

Species

African green monkey

Dilution info

1/1000 - 1/5000

Notes

-

Species

Human

Dilution info

1/1000 - 1/5000

Notes

-

Species

Dog

Dilution info

1/1000 - 1/5000

Notes

-

Predicted
Predicted

Species

Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Turkey, Pig, Duck

Dilution info

-

Notes

-

Target data

Function

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively (PubMed:3170585, PubMed:11724794). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate (PubMed:3170585, PubMed:11724794). Modulates the organization and assembly of the cytoskeleton (By similarity). Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes (PubMed:23071094). Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (PubMed:23071094). Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (PubMed:23332158, PubMed:27387501). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis (By similarity). Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity).

Alternative names

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Rabbit Polyclonal GAPDH antibody. Suitable for WB and reacts with Mouse, Rat, African green monkey, Human, Dog samples. Cited in 114 publications. Immunogen corresponding to Synthetic Peptide within Human GAPDH aa 100-150.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human GAPDH aa 100-150. The exact immunogen used to generate this antibody is proprietary information. Database link P04406
Purification technique

Affinity purification Immunogen

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological function summary

GAPDH serves important metabolic functions beyond its enzymatic role in glycolysis. It functions as part of a multi-enzyme complex within the cytoplasm which facilitates efficient substrate channeling during glycolysis. Additionally GAPDH has non-glycolytic roles including involvement in nuclear processes like RNA export and DNA repair. Its ubiquitous presence across different cellular compartments indicates its multiple functions beyond metabolic pathways.

Activity summary

Glyceraldehyde-3-phosphate dehydrogenase commonly known as GAPDH is an enzyme involved in glycolysis. Its molecular weight (MW) is approximately 36 kDa. The protein is expressed ubiquitously in almost all tissues reflecting its essential role in energy production. GAPDH catalyzes the sixth step of glycolysis converting glyceraldehyde-3-phosphate into 13-bisphosphoglycerate. Due to its stable expression researchers often use GAPDH as a loading control in western blot experiments.

Pathways

GAPDH integrates into significant cellular functions like the glycolytic pathway and apoptotic pathways. In glycolysis GAPDH collaborates with enzymes like phosphoglycerate kinase forming a cohesive link in the energy conversion chain. Its participation in apoptotic pathways highlights GAPDH's involvement in cellular death processes interacting with proteins like Bcl-2 to influence apoptosis progression. These roles reinforce its presence in central metabolic and regulatory pathways.

Associated diseases and disorders

GAPDH has associations with neurodegenerative diseases and cancer. In neurodegenerative disorders such as Alzheimer's disease GAPDH’s altered enzymatic activity is frequently observed influencing cellular energy homeostasis. Moreover overexpression or aberrant regulation of GAPDH relates to cancer cell proliferation and metastasis implicating proteins like p53 in these pathways. The diverse functions and interactions of GAPDH emphasize its importance in both normal cellular function and disease states.

Product promise

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2 product images

  • Western blot - Anti-GAPDH antibody - Loading Control (ab22555), expandable thumbnail

    Western blot - Anti-GAPDH antibody - Loading Control (ab22555)

    Lane 1: Western blot - Anti-GAPDH antibody - Loading Control (AB22555)

    Lanes 2 - 4: Western blot - Anti-GAPDH antibody - Loading Control (AB22555) at 1/1000 dilution

    Lane 1: HeLa cell lysate at 25 µg

    Lane 2: 293 cell lysate at 25 µg

    Lane 3: HUVEC cell lysate at 25 µg

    Lane 4: PC12 cell lysate at 25 µg

    Predicted band size: 36 kDa

  • Western blot - Anti-GAPDH antibody - Loading Control (ab22555), expandable thumbnail

    Western blot - Anti-GAPDH antibody - Loading Control (ab22555)

    All lanes: Western blot - Anti-GAPDH antibody - Loading Control (AB22555) at 1/1000 dilution

    Lane 1: A431 cell lysate at 30 µg

    Lane 2: COS-7 cell lysate at 30 µg

    Lane 3: MDCK cell lysate at 30 µg

    Lane 4: PC-3 cell lysate at 30 µg

    Lane 5: Mouse brain tissue lysate at 30 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG (H+L) Superclonal™ HRP conjugate at 1/4000 dilution

    Predicted band size: 36 kDa

    Observed band size: 37 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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