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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
Learn about all product ranges with our product overviews.
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Rabbit Polyclonal GAPDH antibody. Suitable for WB and reacts with Mouse, Rat, African green monkey, Human, Dog samples. Cited in 114 publications. Immunogen corresponding to Synthetic Peptide within Human GAPDH aa 100-150.
IgG
Rabbit
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
Liquid
Polyclonal
WB | |
---|---|
Human | Tested |
Mouse | Tested |
Rat | Tested |
African green monkey | Tested |
Cat | Predicted |
Chicken | Predicted |
Cow | Predicted |
Dog | Tested |
Duck | Predicted |
Guinea pig | Predicted |
Horse | Predicted |
Pig | Predicted |
Rabbit | Predicted |
Sheep | Predicted |
Turkey | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 - 1/5000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/5000 | Notes - |
Species African green monkey | Dilution info 1/1000 - 1/5000 | Notes - |
Species Human | Dilution info 1/1000 - 1/5000 | Notes - |
Species Dog | Dilution info 1/1000 - 1/5000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Turkey, Pig, Duck | Dilution info - | Notes - |
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively (PubMed:3170585, PubMed:11724794). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate (PubMed:3170585, PubMed:11724794). Modulates the organization and assembly of the cytoskeleton (By similarity). Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes (PubMed:23071094). Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (PubMed:23071094). Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (PubMed:23332158, PubMed:27387501). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis (By similarity). Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity).
Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH, OK/SW-cl.12, CDABP0047, GAPD, GAPDH
Rabbit Polyclonal GAPDH antibody. Suitable for WB and reacts with Mouse, Rat, African green monkey, Human, Dog samples. Cited in 114 publications. Immunogen corresponding to Synthetic Peptide within Human GAPDH aa 100-150.
Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH, OK/SW-cl.12, CDABP0047, GAPD, GAPDH
IgG
Rabbit
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
GAPDH serves important metabolic functions beyond its enzymatic role in glycolysis. It functions as part of a multi-enzyme complex within the cytoplasm which facilitates efficient substrate channeling during glycolysis. Additionally GAPDH has non-glycolytic roles including involvement in nuclear processes like RNA export and DNA repair. Its ubiquitous presence across different cellular compartments indicates its multiple functions beyond metabolic pathways.
Glyceraldehyde-3-phosphate dehydrogenase commonly known as GAPDH is an enzyme involved in glycolysis. Its molecular weight (MW) is approximately 36 kDa. The protein is expressed ubiquitously in almost all tissues reflecting its essential role in energy production. GAPDH catalyzes the sixth step of glycolysis converting glyceraldehyde-3-phosphate into 13-bisphosphoglycerate. Due to its stable expression researchers often use GAPDH as a loading control in western blot experiments.
GAPDH integrates into significant cellular functions like the glycolytic pathway and apoptotic pathways. In glycolysis GAPDH collaborates with enzymes like phosphoglycerate kinase forming a cohesive link in the energy conversion chain. Its participation in apoptotic pathways highlights GAPDH's involvement in cellular death processes interacting with proteins like Bcl-2 to influence apoptosis progression. These roles reinforce its presence in central metabolic and regulatory pathways.
GAPDH has associations with neurodegenerative diseases and cancer. In neurodegenerative disorders such as Alzheimer's disease GAPDH’s altered enzymatic activity is frequently observed influencing cellular energy homeostasis. Moreover overexpression or aberrant regulation of GAPDH relates to cancer cell proliferation and metastasis implicating proteins like p53 in these pathways. The diverse functions and interactions of GAPDH emphasize its importance in both normal cellular function and disease states.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Full details and terms and conditions can be found here:
Terms & Conditions.
Lane 1: Western blot - Anti-GAPDH antibody - Loading Control (AB22555)
Lanes 2 - 4: Western blot - Anti-GAPDH antibody - Loading Control (AB22555) at 1/1000 dilution
Lane 1: HeLa cell lysate at 25 µg
Lane 2: 293 cell lysate at 25 µg
Lane 3: HUVEC cell lysate at 25 µg
Lane 4: PC12 cell lysate at 25 µg
Predicted band size: 36 kDa
All lanes: Western blot - Anti-GAPDH antibody - Loading Control (AB22555) at 1/1000 dilution
Lane 1: A431 cell lysate at 30 µg
Lane 2: COS-7 cell lysate at 30 µg
Lane 3: MDCK cell lysate at 30 µg
Lane 4: PC-3 cell lysate at 30 µg
Lane 5: Mouse brain tissue lysate at 30 µg
All lanes: Goat anti-Rabbit IgG (H+L) Superclonal™ HRP conjugate at 1/4000 dilution
Predicted band size: 36 kDa
Observed band size: 37 kDa
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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