AB70699

Anti-GAPDH antibody - Loading Control

Lab Essentials
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(68 Publications)

Rabbit Polyclonal GAPDH antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 68 publications. Immunogen corresponding to Synthetic Peptide within Human GAPDH aa 250 to C-terminus.

View Alternative Names

GAPD, CDABP0047, OK/SW-cl.12, GAPDH, Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH

3 Images

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody - Loading Control (AB70699)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling GAPDH with ab70699 at 1/200 (1μg/ml). Detection : DAB.

Unknown

Western blot - Anti-GAPDH antibody - Loading Control (AB70699)

All lanes:

Western blot - Anti-GAPDH antibody - Loading Control (ab70699) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HEK293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

TCMK-1 whole cell lysate at 50 µg

Lane 5:

NIH 3T3 whole cell lysate at 50 µg

Predicted band size: 36 kDa

false

Unknown

Western blot - Anti-GAPDH antibody - Loading Control (AB70699)

All lanes:

Western blot - Anti-GAPDH antibody - Loading Control (ab70699) at 1/2000 dilution

Lane 1:

Western blot - Recombinant human GAPDH protein (Active) (<a href='/en-us/products/proteins-peptides/recombinant-human-gapdh-protein-active-ab82633'>ab82633</a>) at 1 µg

Lane 2:

Western blot - Recombinant human GAPDH protein (Active) (<a href='/en-us/products/proteins-peptides/recombinant-human-gapdh-protein-active-ab82633'>ab82633</a>) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 36 kDa

true

Exposure time: 150s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human GAPDH aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P04406

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Guinea pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Lizard": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Turkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glyceraldehyde-3-phosphate dehydrogenase commonly known as GAPDH is an enzyme involved in glycolysis. Its molecular weight (MW) is approximately 36 kDa. The protein is expressed ubiquitously in almost all tissues reflecting its essential role in energy production. GAPDH catalyzes the sixth step of glycolysis converting glyceraldehyde-3-phosphate into 13-bisphosphoglycerate. Due to its stable expression researchers often use GAPDH as a loading control in western blot experiments.

Biological function summary

GAPDH serves important metabolic functions beyond its enzymatic role in glycolysis. It functions as part of a multi-enzyme complex within the cytoplasm which facilitates efficient substrate channeling during glycolysis. Additionally GAPDH has non-glycolytic roles including involvement in nuclear processes like RNA export and DNA repair. Its ubiquitous presence across different cellular compartments indicates its multiple functions beyond metabolic pathways.

Pathways

GAPDH integrates into significant cellular functions like the glycolytic pathway and apoptotic pathways. In glycolysis GAPDH collaborates with enzymes like phosphoglycerate kinase forming a cohesive link in the energy conversion chain. Its participation in apoptotic pathways highlights GAPDH's involvement in cellular death processes interacting with proteins like Bcl-2 to influence apoptosis progression. These roles reinforce its presence in central metabolic and regulatory pathways.

GAPDH has associations with neurodegenerative diseases and cancer. In neurodegenerative disorders such as Alzheimer's disease GAPDH’s altered enzymatic activity is frequently observed influencing cellular energy homeostasis. Moreover overexpression or aberrant regulation of GAPDH relates to cancer cell proliferation and metastasis implicating proteins like p53 in these pathways. The diverse functions and interactions of GAPDH emphasize its importance in both normal cellular function and disease states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the conversion of D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate in glycolysis and the reverse reaction in gluconeogenesis (PubMed : 11724794, PubMed : 3170585). Also shows nitrosylase activity, thereby playing a role in nuclear functions (PubMed : 11724794, PubMed : 3170585). Modulates the organization and assembly of the cytoskeleton (By similarity). Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes (PubMed : 23071094). Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (PubMed : 23071094). Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (PubMed : 23332158, PubMed : 27387501). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis (By similarity). Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity).

See full target information GAPDH

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Publications (68)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 6:102155 PubMed40449482

2025

Ultrabright ratiometric Raman-guided epilepsy surgery by intraoperatively visualizing proinflammatory microglia.

Applications

Unspecified application

Species

Unspecified reactive species

Cong Wang,Zhi Li,Xiao Zhu,Wanbing Sun,Yue Ding,Wenjia Duan,Difei Wang,Yiqing Jiang,Ming Chen,Yuncan Chen,Jiayi Hu,Zheping Cai,Jing Zhao,Junfeng Wang,Zhen Fan,Faming Zheng,Xingyu Zhou,Fang Xie,Jianping Zhang,Yihui Guan,Kui Yan,Zuhai Lei,Qinyue Wang,Luting Wang,Xiao Xiao,Hairong Zheng,Liang Chen,Cong Li,Ying Mao

Frontiers in cellular neuroscience 19:1577117 PubMed40271540

2025

A humanized Gs-coupled DREADD for circuit and behavior modulation.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Zhang,Ruiqi Wang,Liang Zhang,Mengqi Li,Jianbang Lin,Xiaoyang Lu,Yixuan Tian,Yunping Lin,Taian Liu,Yefei Chen,Yuantao Li,Jun Cao,Qiang Wu,Jinhui Wang,Zhonghua Lu,Zexuan Hong

Journal of cellular and molecular medicine 29:e70522 PubMed40133221

2025

TAB2 Promotes Immune Escape and Chemoresistance Through NF-κB Pathway Activation in Cervical Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Man Wu,Yingying Zhang,Xuanhui Wang,Yijia Zhou

MedComm 5:e568 PubMed38756440

2024

Neurotransmitter accumulation and Parkinson's disease-like phenotype caused by anion channelrhodopsin opto-controlled astrocytic mitochondrial depolarization in substantia nigra pars compacta.

Applications

Unspecified application

Species

Unspecified reactive species

Sen-Miao Li,Dian-Dian Wang,Dan-Hua Liu,Xiao-Yan Meng,Zhizhong Wang,Xitong Guo,Qian Liu,Pei-Pei Liu,Shu-Ang Li,Songwei Wang,Run-Zhou Yang,Yuming Xu,Longde Wang,Jian-Sheng Kang

Journal of gastrointestinal oncology 14:2178-2191 PubMed37969822

2023

HMGB2 upregulation promotes the progression of hepatocellular carcinoma cells through the activation of ZEB1/vimentin axis.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Lu,Teng Zhao,Lixue Yang,Yang Liu,Xiang Ruan,Longjiu Cui,Yongjie Zhang

Journal of biochemical and molecular toxicology 37:e23410 PubMed37393518

2023

Circ_0005615 promotes cervical cancer cell growth and metastasis by modulating the miR-138-5p/KDM2A axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoning Luo,Jiewen Liu,Xiangcai Wang,Yili Wang,Jun Yuan,Yu Zhang

iScience 26:106855 PubMed37275520

2023

Antagonistic interactions among structured domains in the multivalent Bicc1-ANKS3-ANKS6 protein network govern phase transitioning of target mRNAs.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin Rothé,Simon Fortier,Céline Gagnieux,Céline Schmuziger,Daniel B Constam

Journal of medical virology 95:e28706 PubMed36971141

2023

Integrative bulk and single-cell transcriptome profiling analysis reveals IFI27 as a novel interferon-stimulated gene in dengue.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Jiang,Cong He,Jing Kan,Huiting Guan,Tao Zhou,Yi Yang

Frontiers in pharmacology 13:1024292 PubMed36483736

2022

HJ11 decoction restrains development of myocardial ischemia-reperfusion injury in rats by suppressing ACSL4-mediated ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Fangyuan Zhang,Ziyun Li,Ping Gao,Jiaxi Zou,Yuting Cui,Yi Qian,Renjun Gu,Weiming Xu,Jingqing Hu

Antioxidants (Basel, Switzerland) 11: PubMed36009300

2022

Tolerance Assessment of Polysaccharide in the Diet of Largemouth Bass ().

Applications

Unspecified application

Species

Unspecified reactive species

Bo Dong,Liyun Wu,Qiaozhen Chen,Wenjie Xu,Dinggang Li,Dong Han,Xiaoming Zhu,Haokun Liu,Yunxia Yang,Shouqi Xie,Junyan Jin

View all publications

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