Anti-GAPDH antibody - Loading Control

• WB

Lab

Western blot - Anti-GAPDH antibody - Loading Control (AB83956)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab83956 overnight at 4°C. Antibody binding was detected using ab175779 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-GAPDH antibody - Loading Control (ab83956) at 1 µg/mL

All lanes:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Chicken IgY H&L (Alexa Fluor® 680) (<a href='/en-us/products/secondary-antibodies/goat-chicken-igy-h-l-alexa-fluor-680-ab175779'>ab175779</a>) at 1/10000 dilution

Observed band size: 37 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody - Loading Control (AB83956)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab83956 overnight at 4°C. Antibody binding was detected using ab175754 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-GAPDH antibody - Loading Control (ab83956) at 1 µg/mL

All lanes:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Chicken IgY H&L (Alexa Fluor® 750) (<a href='/en-us/products/secondary-antibodies/goat-chicken-igy-h-l-alexa-fluor-750-ab175754'>ab175754</a>) at 1/10000 dilution

Observed band size: 37 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody - Loading Control (AB83956)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab83956 overnight at 4°C. Antibody binding was detected using ab175755 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-GAPDH antibody - Loading Control (ab83956) at 1 µg/mL

All lanes:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Chicken IgY H&L (Alexa Fluor® 750) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-chicken-igy-h-l-alexa-fluor-750-preadsorbed-ab175755'>ab175755</a>) at 1/10000 dilution

Observed band size: 37 kDa

false

• WB

Lab

Western blot - Anti-GAPDH antibody - Loading Control (AB83956)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab83956 overnight at 4°C. Antibody binding was detected using ab175787 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-GAPDH antibody - Loading Control (ab83956) at 1 µg/mL

All lanes:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Chicken IgY H&L (Alexa Fluor® 790) (<a href='/en-us/products/secondary-antibodies/goat-chicken-igy-h-l-alexa-fluor-790-ab175787'>ab175787</a>) at 1/10000 dilution

Observed band size: 37 kDa

false

• WB

Unknown

Western blot - Anti-GAPDH antibody - Loading Control (AB83956)

All lanes:

Western blot - Anti-GAPDH antibody - Loading Control (ab83956) at 1 µg/mL

All lanes:

HeLa Cell Lysate at 15 µg

Predicted band size: 36 kDa

Observed band size: 42 kDa

false