Anti-GAPDH antibody - Loading Control is a rabbit polyclonal antibody that is used to detect GAPDH in ICC/IF, IHC-P, Western blot. Suitable for Human, Mouse samples.
- Trusted since 2002
Images
Publications
Filter by
- The Journal of neuroscience : the official journal of the Society for Neuroscience 44:2024ZCCHC17 Modulates Neuronal RNA Splicing and Supports Cognitive Resilience in Alzheimer's Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnne Marie W Bartosch,Elliot H H Youth,Shania Hansen,Yiyang Wu,Heather M Buchanan,Maria E Kaufman,Harrison Xiao,So Yeon Koo,Archana Ashok,Sharanya Sivakumar,Rajesh K Soni,Logan C Dumitrescu,Tiffany G Lam,Ali S Ropri,Annie J Lee,Hans-Ulrich Klein,Badri N Vardarajan,David A Bennett,Tracy L Young-Pearse,Philip L De Jager,Timothy J Hohman,Andrew A Sproul,Andrew F TeichPubMed 38050142
- Experimental and therapeutic medicine 27:402023Icariside II protects dopaminergic neurons from 1‑methyl‑4‑phenylpyridinium‑induced neurotoxicity by downregulating HDAC2 to restore mitochondrial function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenbo Fan,Jianwu ZhouPubMed 38125349
- Bone research 11:642023Sensory nerves directly promote osteoclastogenesis by secreting peptidyl-prolyl cis-trans isomerase D (Cyp40).Applications:Unspecified applicationReactive species:Unspecified reactive speciesJunqin Li,Bin Liu,Hao Wu,Shuaishuai Zhang,Zhuowen Liang,Shuo Guo,Huijie Jiang,Yue Song,Xing Lei,Yi Gao,Pengzhen Cheng,Donglin Li,Jimeng Wang,Yang Liu,Di Wang,Nazhi Zhan,Jing Xu,Lin Wang,Guozhi Xiao,Liu Yang,GuoXian PeiPubMed 38097598
- Nature metabolism 5:2111-21302023Iron accumulation drives fibrosis, senescence and the senescence-associated secretory phenotype.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMate Maus,Vanessa López-Polo,Lidia Mateo,Miguel Lafarga,Mònica Aguilera,Eugenia De Lama,Kathleen Meyer,Anna Sola,Cecilia Lopez-Martinez,Ines López-Alonso,Marc Guasch-Piqueras,Fernanda Hernandez-Gonzalez,Selim Chaib,Miguel Rovira,Mayka Sanchez,Rosa Faner,Alvar Agusti,Rodrigo Diéguez-Hurtado,Sagrario Ortega,Anna Manonelles,Stefan Engelhardt,Freddy Monteiro,Camille Stephan-Otto Attolini,Neus Prats,Guillermo Albaiceta,Josep M Cruzado,Manuel SerranoPubMed 38097808
- Cellular and molecular biology (Noisy-le-Grand, France) 69:1-72023Transcriptional factor CCAAT enhancer binding protein beta inhibits epithelial-mesenchymal transition in cervical cancer via regulating attractin-like 1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMinjie Fang,Yayan Zhou,Zihuang Li,Xianming LiPubMed 38158696
- Acta biochimica Polonica 70:985-9902023Protective effect of tretinoin derivative and TXNRD1 protein on streptozotocin induced gestational diabetes via an age-rage signaling-pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWensheng Wang,Lin WangPubMed 38060807
- eLife 12:2023Apoptotic signaling clears engineered in an organ-specific manner.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTaylor J Abele,Zachary P Billman,Lupeng Li,Carissa K Harvest,Alexia K Bryan,Gabrielle R Magalski,Joseph P Lopez,Heather N Larson,Xiao-Ming Yin,Edward A MiaoPubMed 38055781
- Neoplasma 70:597-6092023RNASEH1-AS1 induced by H3K27ac stabilizes ANXA2 mRNA to promote the progression of colorectal cancer through recruiting BUD13.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShengwei Zhuang,Weihong Lu,Lianjun Shen,Zhekun Huang,Xiuping Zhang,Yong ZhangPubMed 38053379
- Nature communications 14:77622023SMARCB1 loss activates patient-specific distal oncogenic enhancers in malignant rhabdoid tumors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNing Qing Liu,Irene Paassen,Lars Custers,Peter Zeller,Hans Teunissen,Dilara Ayyildiz,Jiayou He,Juliane Laura Buhl,Eelco Wieger Hoving,Alexander van Oudenaarden,Elzo de Wit,Jarno DrostPubMed 38040699
- Acta cirurgica brasileira 38:e3858232023Saffron reduces the liver fibrosis in mice by inhibiting the JAK/STAT3 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLijuan Huang,Yan Han,Zhi Wang,Qiao Qiu,Sichen Yue,Qingmin Zhou,Wei Su,Jianhui YanPubMed 38055395
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Consider this alternative
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Expected |
| Mouse | Expected | Tested | Expected |
| Rat | Predicted | Predicted | Predicted |
| African green monkey | Predicted | Predicted | Predicted |
| Chicken | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted |
| Saccharomyces cerevisiae | Predicted | Predicted | Predicted |
| Schizosaccharomyces pombe | Predicted | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Chicken, Dog, Saccharomyces cerevisiae, Xenopus laevis, Schizosaccharomyces pombe, African green monkey | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2500 | Notes Some customers have experienced that milk significantly decreases the signal in WB compared to BSA. In-house we use BSA. We recommend Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) ab175781) secondary antibody. |
Species Mouse | Dilution info 1/25000 | Notes Some customers have experienced that milk significantly decreases the signal in WB compared to BSA. In-house we use BSA. We recommend Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) ab175781) secondary antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Chicken, Dog, Saccharomyces cerevisiae, Xenopus laevis, Schizosaccharomyces pombe, African green monkey | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 5 µg/mL | Notes We recommend Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody. |
Species Human | Dilution info 5 µg/mL | Notes We recommend Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Chicken, Dog, Saccharomyces cerevisiae, Xenopus laevis, Schizosaccharomyces pombe, African green monkey | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
6 products for Alternative Product
Target data
Function
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively (PubMed:11724794, PubMed:3170585). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate (PubMed:11724794, PubMed:3170585). Modulates the organization and assembly of the cytoskeleton (By similarity). Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes (PubMed:23071094). Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (PubMed:23071094). Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (PubMed:23332158, PubMed:27387501). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis (By similarity). Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity).
Alternative names
GAPD, CDABP0047, OK/SW-cl.12, GAPDH, Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH
Recommended products
Anti-GAPDH antibody - Loading Control is a rabbit polyclonal antibody that is used to detect GAPDH in ICC/IF, IHC-P, Western blot. Suitable for Human, Mouse samples.
- Trusted since 2002
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Protein A
- Specificity
From Mar 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab313650.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-GAPDH antibody - Loading Control (ab9485) is a rabbit polyclonal antibody and is validated for use in ICC/IF, IHC-P and WB.
Anti-GAPDH antibody - Loading Control (ab9485) was first used in a scientific publication in 2004 and has been cited over 3283 times in peer reviewed journals. It's performance in Western blot in human, mouse and rat samples is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-GAPDH antibody - Loading Control (ab9485) has high sensitivity and specificity.
Anti-GAPDH antibody - Loading Control (ab9485) has 87 independent reviews from customers.
GAPDH antibodies are often used as loading controls in Western Blot. Anti-GAPDH antibody - Loading Control has been verified in Western Blot samples and detects a band at 36kDa Molecular weight.
Anti-GAPDH antibody - Loading Control (ab9485) specifically detects GAPDH (UniProt ID: P04406; Molecular weight: 36kDa) and is sold in 100 µg and 250 µg selling sizes.
One of the top cited antibody in the market for GAPDH with >4500 citations and >60 five star reviews. GAPDH is a key target involved in glycolysis and cell metabolism. It plays a crucial role as a housekeeping gene, particularly in understanding GAPDH expression and its function as a metabolic enzyme. GAPDH is widely analysed in GAPDH activity assays and studies of its role in various cellular processes. Additionally, GAPDH is commonly used as a loading control in western blotting, IHC and immunofluorescence experiments.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Glyceraldehyde-3-phosphate dehydrogenase commonly known as GAPDH is an enzyme involved in glycolysis. Its molecular weight (MW) is approximately 36 kDa. The protein is expressed ubiquitously in almost all tissues reflecting its essential role in energy production. GAPDH catalyzes the sixth step of glycolysis converting glyceraldehyde-3-phosphate into 13-bisphosphoglycerate. Due to its stable expression researchers often use GAPDH as a loading control in western blot experiments.
- Biological function summary
GAPDH serves important metabolic functions beyond its enzymatic role in glycolysis. It functions as part of a multi-enzyme complex within the cytoplasm which facilitates efficient substrate channeling during glycolysis. Additionally GAPDH has non-glycolytic roles including involvement in nuclear processes like RNA export and DNA repair. Its ubiquitous presence across different cellular compartments indicates its multiple functions beyond metabolic pathways.
- Pathways
GAPDH integrates into significant cellular functions like the glycolytic pathway and apoptotic pathways. In glycolysis GAPDH collaborates with enzymes like phosphoglycerate kinase forming a cohesive link in the energy conversion chain. Its participation in apoptotic pathways highlights GAPDH's involvement in cellular death processes interacting with proteins like Bcl-2 to influence apoptosis progression. These roles reinforce its presence in central metabolic and regulatory pathways.
- Associated diseases and disorders
GAPDH has associations with neurodegenerative diseases and cancer. In neurodegenerative disorders such as Alzheimer's disease GAPDH’s altered enzymatic activity is frequently observed influencing cellular energy homeostasis. Moreover overexpression or aberrant regulation of GAPDH relates to cancer cell proliferation and metastasis implicating proteins like p53 in these pathways. The diverse functions and interactions of GAPDH emphasize its importance in both normal cellular function and disease states.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
9 product images
Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (ab9485)
ab9485 staining GAPDH in HeLa cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab9485 at 5μg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1μg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody at 2 μg/ml (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) secondary antibody at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labelled in blue with DAPI.
Negative controls: 1– Rabbit primary antibody and anti-mouse secondary antibody; 2 – Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.
Western blot - Anti-GAPDH antibody - Loading Control (ab9485)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab9485 overnight at 4°C. Antibody binding was detected using Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) ab175781) secondary antibody at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
All lanes: Western blot - Anti-GAPDH antibody - Loading Control (ab9485) at 1/2500 dilution
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Lane 2: A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Lane 3: A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) ab175781) secondary antibody at 1/10000 dilution
Predicted band size: 36 kDa
Observed band size: 37 kDa
Image from Wu T et al., PLoS One, 14(4), Fig 3.; doi: 10.1371/journal.pone.0216042. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.Western blot - Anti-GAPDH antibody - Loading Control (ab9485)
HEK293 cells stably transfected with pINDUCER10-shNF90/NF110 (D2) or pINDUCER10-shNF45 (D5) were treated without or with doxycycline for 96 h, then serum starved for 12 h and treated with PMA (20 ng/mL) for 2 h. Protein lysates (20 μg/lane) were separated by SDS-PAGE and transferred to PVDF membranes.
Loading control: Rabbit polyclonal to GAPDH (ab9485) at 1/1000 dilution.
Secondary antibodies (HRP) were used at 1/10,000 dilution.
All lanes: Western blot - Anti-GAPDH antibody - Loading Control (ab9485)
Predicted band size: 36 kDa
Observed band size: 37 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody - Loading Control (ab9485)
IHC image of ab9485 staining GAPDH in human pancreas formalin fixed paraffin embedded tissue sections*, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9485, 5μg/ml working concentration, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the secondary only control (shown on the inset).
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Western blot - Anti-GAPDH antibody - Loading Control (ab9485)
Western blot image using 4-20% Optiblot gel with the Prism Ultra Protein Ladder (Prestained Protein Ladder - Broad molecular weight (10 - 245 kDa) ab116028) 5μl used. We recommend using our ECL substrate kit (ECL Western Blotting Substrate Kit ab65623).
20ug of Lysate per lane and detection using ab9485 diluted to 1ug/ml.
Lane 1: HeLa cell lysate
Lane 2: Jurkat cell lysate
Lane 3: A431 cell lysate
Lane 4: HEK-293 cell lysate
Lane 5: HepG2 cell lysate.All lanes: Western blot - Anti-GAPDH antibody - Loading Control (ab9485) at 1 µg/mL
Lane 1: HeLa cell lysate at 20 µg
Lane 2: Jurkat cell lysate at 20 µg
Lane 3: A431 cell lysate at 20 µg
Lane 4: HEK-293 cell lysate at 20 µg
Lane 5: HepG2 cell lysate at 20 µg
Predicted band size: 36 kDa
This image is courtesy of an anonymous customer reviewWestern blot - Anti-GAPDH antibody - Loading Control (ab9485)
Primary incubation: 16 hours at 4°CBlocking: 5% milk for 1 hour at room temperature
All lanes: Western blot - Anti-GAPDH antibody - Loading Control (ab9485) at 1/1000 dilution
Lane 1: Mouse hepatocytes - untreated at 20 µg
Lane 2: Mouse hepatocytes - treated with LPS (100 ng/mL) for 1 hour at 20 µg
Lane 3: Mouse hepatocytes - treated with LPS (100 ng/mL) for 12 hours at 20 µg
SecondaryAll lanes: Goat anti-rabbit secondary antibody (HRP) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 37 kDa
Exposure time: 1min
This image is a courtesy of Anonymous customer reviewWestern blot - Anti-GAPDH antibody - Loading Control (ab9485)
All lanes: Western blot - Anti-GAPDH antibody - Loading Control (ab9485) at 1/2500 dilution
Lane 1: Lysate prepared from human Huh-7 cells at 2 µg
Lane 2: Lysate prepared from human Huh-7 cells at 20 µg
SecondaryAll lanes: HRP-conjugated sheep polyclonal to rabbit IgG at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 40 kDa
Exposure time: 5min
Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (ab9485)
ab9485 staining GAPDH in NIH3T3 cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab9485 at 5μg/ml and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 at 1/250 overnight at +4°C, followed by a further incubation at room temperature for 1h with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Western blot - Anti-GAPDH antibody - Loading Control (ab9485)
All lanes: Western blot - Anti-GAPDH antibody - Loading Control (ab9485) at 1/1000 dilution
All lanes: Mouse Embryonic lung whole tissue lysate at 30 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Exposure time: 15s
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com