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Anti-GAPDH antibody [mAbcam 9484] ab9484 is a mouse monoclonal antibody that is used in GAPDH western blotting and IHC. Suitable for human, mouse and rat samples.

- Antibody clone mAbcam 9484 has been tried and trusted by researchers since 2004 and is cited in >1160 publications
Same trusted quality, new lower price


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [mAbcam 9484] - Loading Control (AB9484), expandable thumbnail
  • Western blot - Anti-GAPDH antibody [mAbcam 9484] - Loading Control (AB9484), expandable thumbnail
  • Western blot - Anti-GAPDH antibody [mAbcam 9484] - Loading Control (AB9484), expandable thumbnail
  • Western blot - Anti-GAPDH antibody [mAbcam 9484] - Loading Control (AB9484), expandable thumbnail
  • Western blot - Anti-GAPDH antibody [mAbcam 9484] - Loading Control (AB9484), expandable thumbnail

Publications

Key facts

Isotype

IgG2b

Host species

Mouse

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested
Mouse
Tested
Expected
Rat
Tested
Expected
Chicken
Tested
Expected
Chinese hamster
Tested
Expected
Cow
Tested
Expected
Cynomolgus monkey
Predicted
Predicted
Dog
Predicted
Predicted
Pig
Tested
Expected
Rabbit
Predicted
Predicted
Xenopus laevis
Tested
Expected

Tested
Tested

Species

Chinese hamster

Dilution info

0.10000-1.00000 µg/mL

Notes

Do not block with milk. Block with 5% BSA for 1 hour. Our labs have thoroughly investigated the blocking conditions for this antibody. We found that milk significantly decreases the signal and is therefore not a suitable blocking agent for this antibody (see images).

Species

Pig

Dilution info

0.10000-1.00000 µg/mL

Notes

Do not block with milk. Block with 5% BSA for 1 hour. Our labs have thoroughly investigated the blocking conditions for this antibody. We found that milk significantly decreases the signal and is therefore not a suitable blocking agent for this antibody (see images).

Species

Cow

Dilution info

0.10000-1.00000 µg/mL

Notes

Do not block with milk. Block with 5% BSA for 1 hour. Our labs have thoroughly investigated the blocking conditions for this antibody. We found that milk significantly decreases the signal and is therefore not a suitable blocking agent for this antibody (see images).

Species

Chicken

Dilution info

0.10000-1.00000 µg/mL

Notes

Do not block with milk. Block with 5% BSA for 1 hour. Our labs have thoroughly investigated the blocking conditions for this antibody. We found that milk significantly decreases the signal and is therefore not a suitable blocking agent for this antibody (see images).

Species

Xenopus laevis

Dilution info

0.10000-1.00000 µg/mL

Notes

Do not block with milk. Block with 5% BSA for 1 hour. Our labs have thoroughly investigated the blocking conditions for this antibody. We found that milk significantly decreases the signal and is therefore not a suitable blocking agent for this antibody (see images).

Species

Rat

Dilution info

0.10000-1.00000 µg/µL

Notes

Do not block with milk. Block with 5% BSA for 1 hour. Our labs have thoroughly investigated the blocking conditions for this antibody. We found that milk significantly decreases the signal and is therefore not a suitable blocking agent for this antibody (see images).

Species

Mouse

Dilution info

0.10000-1.00000 µg/mL

Notes

Do not block with milk. Block with 5% BSA for 1 hour. Our labs have thoroughly investigated the blocking conditions for this antibody. We found that milk significantly decreases the signal and is therefore not a suitable blocking agent for this antibody (see images).

Species

Human

Dilution info

0.10000-1.00000 µg/mL

Notes

Do not block with milk. Block with 5% BSA for 1 hour. Our labs have thoroughly investigated the blocking conditions for this antibody. We found that milk significantly decreases the signal and is therefore not a suitable blocking agent for this antibody (see images).

Predicted
Predicted

Species

Rabbit, Dog, Cynomolgus monkey

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

5 µg/mL

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species

Chinese hamster, Pig, Cow, Chicken, Xenopus laevis, Rat, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rabbit, Dog, Cynomolgus monkey

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

5 products for Alternative Product

Target data

Function

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively (PubMed:3170585, PubMed:11724794). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate (PubMed:3170585, PubMed:11724794). Modulates the organization and assembly of the cytoskeleton (By similarity). Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes (PubMed:23071094). Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (PubMed:23071094). Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (PubMed:23332158, PubMed:27387501). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis (By similarity). Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity).

Alternative names

Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH, OK/SW-cl.12, CDABP0047, GAPD, GAPDH

Recommended products

Anti-GAPDH antibody [mAbcam 9484] ab9484 is a mouse monoclonal antibody that is used in GAPDH western blotting and IHC. Suitable for human, mouse and rat samples.

- Antibody clone mAbcam 9484 has been tried and trusted by researchers since 2004 and is cited in >1160 publications
Same trusted quality, new lower price

Key facts

Isotype

IgG2b

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

mAbcam 9484

Purity

IgG fraction

Specificity

In western blot, this product typically gives a lower signal in rat lysates compared to human and mouse lysates.

Light chain type

kappa

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

For Western blotting, do not use milk for blocking. Our labs have extensively tested the blocking conditions for this antibody and recommend using 5% BSA for 1 hour. The comparison data is shown in the images section.

This antibody clone [mAbcam 9484] is manufactured by Abcam.

If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Glyceraldehyde-3-phosphate dehydrogenase commonly known as GAPDH is an enzyme involved in glycolysis. Its molecular weight (MW) is approximately 36 kDa. The protein is expressed ubiquitously in almost all tissues reflecting its essential role in energy production. GAPDH catalyzes the sixth step of glycolysis converting glyceraldehyde-3-phosphate into 13-bisphosphoglycerate. Due to its stable expression researchers often use GAPDH as a loading control in western blot experiments.

Biological function summary

GAPDH serves important metabolic functions beyond its enzymatic role in glycolysis. It functions as part of a multi-enzyme complex within the cytoplasm which facilitates efficient substrate channeling during glycolysis. Additionally GAPDH has non-glycolytic roles including involvement in nuclear processes like RNA export and DNA repair. Its ubiquitous presence across different cellular compartments indicates its multiple functions beyond metabolic pathways.

Pathways

GAPDH integrates into significant cellular functions like the glycolytic pathway and apoptotic pathways. In glycolysis GAPDH collaborates with enzymes like phosphoglycerate kinase forming a cohesive link in the energy conversion chain. Its participation in apoptotic pathways highlights GAPDH's involvement in cellular death processes interacting with proteins like Bcl-2 to influence apoptosis progression. These roles reinforce its presence in central metabolic and regulatory pathways.

Associated diseases and disorders

GAPDH has associations with neurodegenerative diseases and cancer. In neurodegenerative disorders such as Alzheimer's disease GAPDH’s altered enzymatic activity is frequently observed influencing cellular energy homeostasis. Moreover overexpression or aberrant regulation of GAPDH relates to cancer cell proliferation and metastasis implicating proteins like p53 in these pathways. The diverse functions and interactions of GAPDH emphasize its importance in both normal cellular function and disease states.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484)

    IHC image of GAPDH staining in human liver FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9484, 5µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  • Western blot - Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484), expandable thumbnail

    Western blot - Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484)

    The membrane 1-5 was blocked in 5% milk (1 hour). The membrane 6-10 was blocked in 5% BSA (1 hour). Milk is not a suitable blocking agent and significantly decreases the signal on the membrane.

    All lanes: Western blot - Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484) at 1/1000 dilution

    Lanes 1 and 6: HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 20 µg

    Lanes 2 and 7: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

    Lanes 3 and 8: Western blot - A-431 whole cell lysate (A-431 whole cell lysate ab7909) at 20 µg

    Lanes 4 and 9: Western blot - Jurkat whole cell lysate (Jurkat whole cell lysate ab7899) at 20 µg

    Lanes 10 and 5: Western blot - HEK-293 whole cell lysate (HEK-293 whole cell lysate ab7902) at 20 µg

    Secondary

    All lanes: Goat anti-Mouse (HRP conjugated) at 1/5000 dilution

    Predicted band size: 36 kDa

    Observed band size: 40 kDa

  • Western blot - Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484), expandable thumbnail

    Western blot - Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484)

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab9484 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406

    All lanes: Western blot - Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484) at 1 µg/mL

    Lane 1: HeLa Whole Cell Lysate at 20 µg

    Lane 2: NIH 3T3 Whole Cell Lysate at 20 µg

    Lane 3: PC12 Whole Cell Lysate at 20 µg

    Secondary

    All lanes: Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 36 kDa

    Exposure time: 1min

  • Western blot - Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484), expandable thumbnail

    Western blot - Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484)

    The membrane was blocked in 5% BSA in TBST for 1 hour, then incubated for 1 hour in primary antibody diluted in TBST.

    All lanes: Western blot - Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484) at 1/5000 dilution

    Lane 1: Hela whole cell (Human)

    Lane 2: 3T3 cell (Mouse)

    Lane 3: Rat brain

    Lane 4: Xenopus laevis embryo

    Lane 5: Chicken Liver

    Lane 6: EBTr cell (Cow)

    Lane 7: CHO cell (Chinese hamster)

    Lane 8: Pig liver

    Secondary

    All lanes: Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (Rabbit Anti-Mouse IgG H&L (HRP) ab6728) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 36 kDa

    Observed band size: 40 kDa

    Exposure time: 10s

  • Western blot - Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484), expandable thumbnail

    Western blot - Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484)

    All lanes: Western blot - Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484) at 0.5 µg/mL

    All lanes: HeLa cell lysate

    Secondary

    All lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) (Goat Anti-Mouse IgG H&L (HRP) ab6789) at 1/5000 dilution

    Developed using the ECL technique.

    Predicted band size: 36 kDa

    Exposure time: 30s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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