Rabbit Polyclonal GAPDS antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GAPDHS aa 150-400.
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Publications
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- Frontiers in pharmacology 11:5474362021EFEMP1 Overexpression Contributes to Neovascularization in Age-Related Macular Degeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLu Cheng,Chong Chen,Wenke Guo,Kun Liu,Qianqian Zhao,Ping Lu,Fudong Yu,Xun XuPubMed 33584252
- Molecular cancer 19:172020Circular RNA MAPK4 (circ-MAPK4) inhibits cell apoptosis via MAPK signaling pathway by sponging miR-125a-3p in gliomas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiehua He et. al.PubMed 31992303
- Molecular medicine reports 19:2471-24782019Aberrant expression of sperm‑specific glycolytic enzymes are associated with poor sperm quality.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuexia Liu et. al.PubMed 30720094
- International journal of oncology 54:7-162018IL-21R functions as an oncogenic factor and is regulated by the lncRNA MALAT1/miR-125a-3p axis in gastric cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLei Yan,Jing Zhang,Dong Guo,Ji Ma,Shao-Feng Shui,Xin-Wei HanPubMed 30387833
- Oncology reports 38:2843-28512017Reversal effect of adenovirus-mediated human interleukin 24 transfection on the cisplatin resistance of A549/DDP lung cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMingju Xu,Xioawei Tang,Jinjin Guo,Wangbang Sun,Faqing TangPubMed 29048638
- Molecular cancer 16:1512017Circular RNA_LARP4 inhibits cell proliferation and invasion of gastric cancer by sponging miR-424-5p and regulating LATS1 expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Zhang et. al.PubMed 28893265
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human GAPDHS aa 150-400. The exact immunogen used to generate this antibody is proprietary information. Database link O14556
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
| Rat | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/10000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
Target data
Function
May play an important role in regulating the switch between different pathways for energy production during spermiogenesis and in the spermatozoon. Required for sperm motility and male fertility (By similarity).
Alternative names
GAPD2, GAPDH2, GAPDS, HSD-35, HSD35, GAPDHS, Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2, Spermatogenic glyceraldehyde-3-phosphate dehydrogenase, GAPDH-2
Recommended products
Rabbit Polyclonal GAPDS antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GAPDHS aa 150-400.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human GAPDHS aa 150-400. The exact immunogen used to generate this antibody is proprietary information. Database link O14556
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GAPDS also known as glyceraldehyde-3-phosphate dehydrogenase-sperm is an important enzyme involved in glycolytic processes. The protein usually references a mass of about 44 kDa. Expressed mainly in testicular cells and spermatozoa GAPDS is important for energy production during spermatogenesis and sperm motility. It shares sequence similarity with GAPDH making it an isoform within the same enzymatic family.
- Biological function summary
GAPDS plays a critical role in glycolysis serving as an important enzyme that facilitates the conversion of glyceraldehyde-3-phosphate to 13-bisphosphoglycerate. GAPDS can function in complex with other glycolytic enzymes helping generate ATP which is necessary for sperm cell functions. GAPDS expression supports processes essential for male fertility specifically through its involvement in energy supply to sperm flagella.
- Pathways
GAPDS is a part of the glycolysis metabolic pathway which is essential for ATP production in sperm. In this pathway it works closely with other glycolytic enzymes like phosphoglycerate kinase and enolase ensuring the continuous flow of energy. GAPDS also indirectly connects to the respiratory chain as glycolytic byproducts feed into mitochondrial oxidative phosphorylation allowing integrated energy metabolism.
- Associated diseases and disorders
GAPDS has implications in male infertility particularly when there is abnormal sperm motility. Defects or deficiencies in GAPDS can impair ATP production in sperm leading to suboptimal motility and reduced fertility. The protein might also have a connection with certain metabolic disorders that affect energy production like glycolytic enzyme deficiencies where similar pathways involving proteins like GAPDH might show concurrent dysfunctions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
Western blot - Anti-GAPDS antibody (ab153802)
10% SDS PAGE
All lanes: Western blot - Anti-GAPDS antibody (ab153802) at 1/1000 dilution
All lanes: MCF7 whole cell lysate at 30 µg
Predicted band size: 45 kDa, 49 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDS antibody (ab153802)
Paraffin embedded human gastric cancer tissue stained for GAPDHS using ab153802 at 1/500 dilution in immunohistochemical analysis.
Western blot - Anti-GAPDS antibody (ab153802)
10% SDS PAGE
All lanes: Western blot - Anti-GAPDS antibody (ab153802) at 1/5000 dilution
All lanes: NIH/3T3 whole cell lysate at 30 µg
Predicted band size: 45 kDa
Immunocytochemistry/ Immunofluorescence - Anti-GAPDS antibody (ab153802)
Immunofluorescent analysis of methanol-fixed MCF7 cells labeling GAPDS with ab153802 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.
Western blot - Anti-GAPDS antibody (ab153802)
10% SDS-PAGE
All lanes: Western blot - Anti-GAPDS antibody (ab153802) at 1/500 dilution
All lanes: Mouse testis tissue extract at 50 µg
Predicted band size: 45 kDa
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