AB86033

Anti-GAPex 5 antibody

Name: Anti-GAPex 5 antibody (ab86033) | Abcam
Brand: Abcam Limited
SKU: AB86033
Price: 495 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal GAPex 5 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human GAPVD1.

View Alternative Names

GAPEX5, KIAA1521, RAP6, GAPVD1, GTPase-activating protein and VPS9 domain-containing protein 1, GAPex-5, Rab5-activating protein 6

2 Images

Unknown

Immunoprecipitation - Anti-GAPex 5 antibody (AB86033)

ab86033, at 0.1 µg/ml, staining GAPex 5 in HeLa whole cell lysate immunoprecipitated using ab86033 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : by chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-GAPex 5 antibody (ab86033)

Predicted band size: 165 kDa

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Unknown

Western blot - Anti-GAPex 5 antibody (AB86033)

All lanes:

Western blot - Anti-GAPex 5 antibody (ab86033) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 165 kDa

Observed band size: 180 kDa,71 kDa

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Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human GAPVD1. The exact immunogen used to generate this antibody is proprietary information.

Q14C86

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Platypus": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Tilapia": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Turkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab86033 was affinity purified using an epitope specific to GAPex 5 immobilized on solid support

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GAPex 5 also known as GTPase-activating protein exchange factor 5 is a protein involved in cellular signaling. It has a mass of approximately 150 kDa and is widely expressed in various tissues. GAPex 5 regulates the activity of GTPases by facilitating the exchange of GDP for GTP which modulates signal transduction pathways. This protein plays an important role in maintaining cellular functions by influencing intracellular signaling processes.

Biological function summary

GAPex 5 interacts with small GTPases and forms part of larger protein complexes impacting different aspects of cell communication and function. It regulates cell growth differentiation and survival by modulating the activity of signaling molecules. GAPex 5's biological activity closely links to the control of cellular proliferation affecting processes such as cell cycle progression and apoptosis. By influencing these processes GAPex 5 strongly contributes to maintaining proper cellular homeostasis.

Pathways

GAPex 5 participates in the MAPK and PI3K/Akt signaling pathways essential for various cellular responses. In the MAPK pathway GAPex 5 interacts with Ras proteins which play an important role in transmitting extracellular signals to the cell nucleus. In the PI3K/Akt pathway its connection to PDK1 influences cell survival and growth. Through these pathways GAPex 5 helps regulate the balance between cell growth and death ensuring appropriate cellular responses to external stimuli.

Dysregulation of GAPex 5 links to cancer and neurodegenerative diseases. In cancer altered GAPex 5 activity correlates with aberrant cell proliferation by affecting Ras proteins. Similarly in neurodegenerative diseases changes in GAPex 5 impact signaling pathways associated with neuronal survival including interactions with proteins like Akt. Understanding the role of GAPex 5 in these disorders may offer insights into potential therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts both as a GTPase-activating protein (GAP) and a guanine nucleotide exchange factor (GEF), and participates in various processes such as endocytosis, insulin receptor internalization or LC2A4/GLUT4 trafficking. Acts as a GEF for the Ras-related protein RAB31 by exchanging bound GDP for free GTP, leading to regulate LC2A4/GLUT4 trafficking. In the absence of insulin, it maintains RAB31 in an active state and promotes a futile cycle between LC2A4/GLUT4 storage vesicles and early endosomes, retaining LC2A4/GLUT4 inside the cells. Upon insulin stimulation, it is translocated to the plasma membrane, releasing LC2A4/GLUT4 from intracellular storage vesicles. Also involved in EGFR trafficking and degradation, possibly by promoting EGFR ubiquitination and subsequent degradation by the proteasome. Has GEF activity for Rab5 and GAP activity for Ras.

See full target information GAPVD1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell science 134: PubMed34657963

2021

His domain protein tyrosine phosphatase and Rabaptin-5 couple endo-lysosomal sorting of EGFR with endosomal maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Gabrielle Parkinson,Peristera Roboti,Ling Zhang,Sandra Taylor,Philip Woodman

PLoS genetics 10:e1004177 PubMed24586203

2014

miR-100 induces epithelial-mesenchymal transition but suppresses tumorigenesis, migration and invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Dahu Chen,Yutong Sun,Yuan Yuan,Zhenbo Han,Peijing Zhang,Jinsong Zhang,M James You,Julie Teruya-Feldstein,Min Wang,Sumeet Gupta,Mien-Chie Hung,Han Liang,Li Ma

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-GAPex 5 antibody

Immunoprecipitation - Anti-GAPex 5 antibody (AB86033)

Western blot - Anti-GAPex 5 antibody (AB86033)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

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