Rabbit Recombinant Monoclonal GARNL1 antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-GARNL1 antibody [EPR13592-38] - BSA and Azide free (AB250623), expandable thumbnail](https://content.abcam.com/products/images/garnl1-antibody-epr13592-38-bsa-and-azide-free-ab250623--immunocytochemistry-immunofluorescence-img95928.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-GARNL1 antibody [EPR13592-38] - BSA and Azide free (AB250623)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GARNL1 antibody [EPR13592-38] - BSA and Azide free (AB250623), expandable thumbnail](https://content.abcam.com/products/images/garnl1-antibody-epr13592-38-bsa-and-azide-free-ab250623--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128423.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GARNL1 antibody [EPR13592-38] - BSA and Azide free (AB250623)")
![Western blot - Anti-GARNL1 antibody [EPR13592-38] - BSA and Azide free (AB250623), expandable thumbnail](https://content.abcam.com/products/images/garnl1-antibody-epr13592-38-bsa-and-azide-free-ab250623--western-blot-img94063.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GARNL1 antibody [EPR13592-38] - BSA and Azide free (AB250623)")
![Western blot - Anti-GARNL1 antibody [EPR13592-38] - BSA and Azide free (AB250623), expandable thumbnail](https://content.abcam.com/products/images/garnl1-antibody-epr13592-38-bsa-and-azide-free-ab250623--western-blot-img95873.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GARNL1 antibody [EPR13592-38] - BSA and Azide free (AB250623)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Catalytic subunit of the heterodimeric RalGAP1 complex which acts as a GTPase activator for the Ras-like small GTPases RALA and RALB.
Alternative names
GARNL1, KIAA0884, TULIP1, RALGAPA1, Ral GTPase-activating protein subunit alpha-1, GAP-related-interacting partner to E12, GTPase-activating Rap/Ran-GAP domain-like 1, Tuberin-like protein 1, p240, GRIPE
Recommended products
Rabbit Recombinant Monoclonal GARNL1 antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR13592-38
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab250623 is the carrier-free version of Anti-GARNL1 antibody [EPR13592-38] ab182570.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GARNL1 also called Rap1-GTPase activating protein 4 functions mechanically as a guanine nucleotide exchange factor (GEF). It specifically activates Rap1 a small GTPase part of the Ras family by facilitating the exchange of GDP for GTP. GARNL1 has a molecular weight of approximately 184 kDa. It is expressed in various tissues including the brain and hematopoietic cells indicating its diverse functional roles across different biological systems.
- Biological function summary
The ability of GARNL1 to modify Rap1 activity influences numerous cellular functions such as cell adhesion growth and differentiation. GARNL1 does its work not as an isolated player but as part of complexes that can include other signaling molecules and scaffolding proteins. By interacting with these complexes GARNL1 contributes to the regulatory mechanisms that maintain cellular homeostasis and responsiveness to extracellular signals.
- Pathways
GARNL1 facilitates signal transduction within pathways like the MAPK/ERK and integrin signaling pathways. Through these pathways GARNL1 works alongside proteins like RAF kinases and integrins contributing to processes like cell migration and proliferation. The interaction with related proteins in these pathways underlines its significance in transmitting signals from the cell membrane to the nucleus influencing gene expression and cell fate decisions.
- Associated diseases and disorders
Mutations or dysregulation of GARNL1 are linked to certain cancers and neurological disorders. In oncogenesis it relates to alterations in cell adhesion and migration potentially collaborating with proteins such as BRAF in promoting tumourigenesis. Regarding neurological conditions abnormalities in GARNL1 function can affect neural connectivity and signaling highlighting its connection to Rap1-related synaptic modulation and signaling in neurons.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunocytochemistry/ Immunofluorescence - Anti-GARNL1 antibody [EPR13592-38] - BSA and Azide free (ab250623), expandable thumbnail](https://content.abcam.com/products/images/garnl1-antibody-epr13592-38-bsa-and-azide-free-ab250623--immunocytochemistry-immunofluorescence-img95928.jpg "Immunocytochemistry/ Immunofluorescence - Anti-GARNL1 antibody [EPR13592-38] - BSA and Azide free (ab250623)")
Immunocytochemistry/ Immunofluorescence - Anti-GARNL1 antibody [EPR13592-38] - BSA and Azide free (ab250623)
This data was developed using Anti-GARNL1 antibody [EPR13592-38] ab182570, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of HeLa cells labeling GARNL1 using Anti-GARNL1 antibody [EPR13592-38] ab182570 at 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor555) at 1/200 dilution was used as secondary antibody. Cells were fixed with -20? Acetone. Counterstain: DAPI.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GARNL1 antibody [EPR13592-38] - BSA and Azide free (ab250623), expandable thumbnail](https://content.abcam.com/products/images/garnl1-antibody-epr13592-38-bsa-and-azide-free-ab250623--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128423.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GARNL1 antibody [EPR13592-38] - BSA and Azide free (ab250623)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GARNL1 antibody [EPR13592-38] - BSA and Azide free (ab250623)
This data was developed using Anti-GARNL1 antibody [EPR13592-38] ab182570, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling GARNL1 using Anti-GARNL1 antibody [EPR13592-38] ab182570 at 1/100 dilution. A Ready to use HRP Polymer for Rabbit IgG was used as a secondary. Counterstain: Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Western blot - Anti-GARNL1 antibody [EPR13592-38] - BSA and Azide free (ab250623), expandable thumbnail](https://content.abcam.com/products/images/garnl1-antibody-epr13592-38-bsa-and-azide-free-ab250623--western-blot-img94063.jpg "Western blot - Anti-GARNL1 antibody [EPR13592-38] - BSA and Azide free (ab250623)")
Western blot - Anti-GARNL1 antibody [EPR13592-38] - BSA and Azide free (ab250623)
This data was developed using Anti-GARNL1 antibody [EPR13592-38] ab182570, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-GARNL1 antibody [EPR13592-38] (Anti-GARNL1 antibody [EPR13592-38] ab182570) at 1/100000 dilution
All lanes: HeLa cell lysate at 1/10 dilution
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 230 kDa
![Western blot - Anti-GARNL1 antibody [EPR13592-38] - BSA and Azide free (ab250623), expandable thumbnail](https://content.abcam.com/products/images/garnl1-antibody-epr13592-38-bsa-and-azide-free-ab250623--western-blot-img95873.jpg "Western blot - Anti-GARNL1 antibody [EPR13592-38] - BSA and Azide free (ab250623)")
Western blot - Anti-GARNL1 antibody [EPR13592-38] - BSA and Azide free (ab250623)
This data was developed using Anti-GARNL1 antibody [EPR13592-38] ab182570, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-GARNL1 antibody [EPR13592-38] (Anti-GARNL1 antibody [EPR13592-38] ab182570) at 1/100000 dilution
All lanes: 293 cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 230 kDa
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