AB196496

Anti-GARS antibody

Name: Anti-GARS antibody (ab196496) | Abcam
Brand: Abcam Limited
SKU: AB196496
Price: 545 USD
Availability: InStock

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Polyclonal GARS antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GARS1.

View Alternative Names

GARS, GARS1, Glycine--tRNA ligase, Diadenosine tetraphosphate synthetase, Glycyl-tRNA synthetase, Glycyl-tRNA synthetase 1, Ap4A synthetase, GlyRS

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-GARS antibody (AB196496)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GARS antibody (AB196496)

Immunofluorescent analysis of MCF7 cells labeling GARS using ab196496 at 1/50 dilution. Blue : DAPI for nuclear staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GARS antibody (AB196496)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GARS antibody (AB196496)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat heart tissue labelling GARS with ab196496 at a dilution of 1/100.

Supplier Data

Western blot - Anti-GARS antibody (AB196496)

All lanes:

Western blot - Anti-GARS antibody (ab196496) at 1/500 dilution

Lane 1:

Jurkat cell lysate

Lane 2:

293T cell lysate

Lane 3:

U87 cell lysate

Lane 4:

Raji cell lysate

Lane 5:

M21 cell lysate

Lane 6:

22RV-1 cell lysate

Lane 7:

HT29 cell lysate

Lane 8:

mouse brain tissue lysate

Lane 9:

mouse spinal cord tissue lysate

Predicted band size: 83 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human GARS1. The exact immunogen used to generate this antibody is proprietary information.

P41250

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glycyl-tRNA synthetase (GARS) also known as GlyRS is an enzyme involved in the protein synthesis process. GARS catalyzes the attachment of glycine to its corresponding transfer RNA (tRNA) enabling the correct integration of glycine into proteins during translation. It has a molecular mass of approximately 79 kDa and is ubiquitously expressed in many tissues including muscle and the nervous system. The enzyme plays a critical function by ensuring the accurate translation of the genetic code into functional proteins.

Biological function summary

GARS maintains the fidelity of protein synthesis ensuring that glycine gets incorporated into proteins correctly. As part of the aminoacyl-tRNA synthetase family it operates within a larger complex important for cellular protein production. GARS ensures that proteins maintain their proper structure and function by providing the correct amino acids during synthesis. Its activity supports cell survival and proper cellular functions across various tissues.

Pathways

The function of GARS is central in aminoacyl-tRNA biosynthesis and the broader protein synthesis pathway. This enzyme works closely with other aminoacyl-tRNA synthetases to ensure the fidelity of protein translation. Interactions with proteins such as EPRS (glutamyl-prolyl-tRNA synthetase) highlight the coordinated effort among different synthetases for efficient protein biosynthesis linking it to a fundamental cellular process essential for cellular viability and function.

Mutations in GARS have been associated with Charcot-Marie-Tooth disease type 2D and distal hereditary motor neuropathy type V. These genetic disorders result in peripheral neuropathy characterized by muscle weakness and sensory deficits. Mutated GARS disrupts normal glycine-tRNA ligation impacting neuronal function. These conditions often involve interactions with proteins like neurofilament proteins contributing to neurodegeneration.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Catalyzes the ATP-dependent ligation of glycine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (Gly-AMP) (PubMed : 17544401, PubMed : 24898252, PubMed : 28675565). Also produces diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs. Thereby, may play a special role in Ap4A homeostasis (PubMed : 19710017).

See full target information GARS1

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Pulmonary circulation 15:e70171 PubMed41070230

2025

MiR-24 Attenuates Oxidative Stress and Mitochondrial Apoptosis in Ventilator-Induced Lung Injury by Targeting Bcl-2-related Ovarian Killer.

Applications

Unspecified application

Species

Unspecified reactive species

Wenbo Xu,Wenjiao Ren,Lingling Zhang,Bing Wang,Linqi Gao,Dong Yuan

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GARS antibody

Immunocytochemistry/ Immunofluorescence - Anti-GARS antibody (AB196496)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GARS antibody (AB196496)

Western blot - Anti-GARS antibody (AB196496)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise